Design and Validation of qPCR-Specific Primers for Quantification of the Marketed Terfezia claveryi and Terfezia crassiverrucosa in Soil
Desert truffle crop is a pioneer in southeastern Spain, a region where native edible hypogeous fungi are adapted to the semiarid areas with low annual rainfall. Terfezia claveryi Chatin was the first species of desert truffle to be cultivated, and has been increasing in recent years as an alternativ...
| Authors: | ; ; |
|---|---|
| Format: | Article |
| Status: | Published version |
| Publication date: | 2022 |
| Country: | España |
| Institution: | Universitat de Lleida (UdL) |
| Repository: | Repositori Obert UdL |
| OAI Identifier: | oai:repositori.udl.cat:10459.1/466357 |
| Online Access: | https://doi.org/10.1016/j.foreco.2022.120602 https://hdl.handle.net/10459.1/466357 |
| Access Level: | Open access |
| Keyword: | ITS Terfezia claveryi Terfezia crassiverrucosa qPCR Desert truffles rDNA Ascomycetes mycelium |
| _version_ | 1878736832427982848 |
|---|---|
| author | Arenas, Francisco |
| author2 | Morte, Asunción Navarro-Ródenas, Alfonso |
| author2_role | author author |
| author_browse | Arenas, Francisco Morte, Asunción Navarro-Ródenas, Alfonso |
| author_facet | Arenas, Francisco Morte, Asunción Navarro-Ródenas, Alfonso |
| author_role | author |
| collection | Repositori Obert UdL |
| description | Desert truffle crop is a pioneer in southeastern Spain, a region where native edible hypogeous fungi are adapted to the semiarid areas with low annual rainfall. Terfezia claveryi Chatin was the first species of desert truffle to be cultivated, and has been increasing in recent years as an alternative rainfed crop in the Iberian Peninsula. However, its behaviour in the field has yet not been investigated. For this purpose, specific primers were designed for the soil DNA quantification of both T. claveryi and Terfezia crassiverrucosa and a real-time qPCR protocol was developed, using the ITS rDNA region as a target. Moreover, a young desert truffle orchard was sampled for environmental validation. The results showed the highest efficiency for the TerclaF3/TerclaR1 primers pair, 89%, and the minimal fungal biomass that could be reliable detected was set at 4.23 µg mycelium/g soil. The spatial distribution of fungal biomass was heterogeneous, and there was not a direct relationship between the quantity of winter soil mycelium and the location/productivity of desert truffles. This protocol could be applied to tracking these species in soil and understand their mycelial dynamics in plantations and wild areas. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_962eeb66a7b3e6b06b2ff800fe6df3fa |
| institution | Universitat de Lleida (UdL) |
| instname_str | Universitat de Lleida (UdL) |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:repositori.udl.cat:10459.1/466357 |
| publishDate | 2022 |
| publishDateSort | 2022 |
| publisher | MDPI |
| publisherStr | MDPI |
| reponame_str | Repositori Obert UdL |
| spelling | Design and Validation of qPCR-Specific Primers for Quantification of the Marketed Terfezia claveryi and Terfezia crassiverrucosa in Soil Arenas, Francisco Morte, Asunción Navarro-Ródenas, Alfonso ITS Terfezia claveryi Terfezia crassiverrucosa qPCR Desert truffles rDNA Ascomycetes mycelium Desert truffle crop is a pioneer in southeastern Spain, a region where native edible hypogeous fungi are adapted to the semiarid areas with low annual rainfall. Terfezia claveryi Chatin was the first species of desert truffle to be cultivated, and has been increasing in recent years as an alternative rainfed crop in the Iberian Peninsula. However, its behaviour in the field has yet not been investigated. For this purpose, specific primers were designed for the soil DNA quantification of both T. claveryi and Terfezia crassiverrucosa and a real-time qPCR protocol was developed, using the ITS rDNA region as a target. Moreover, a young desert truffle orchard was sampled for environmental validation. The results showed the highest efficiency for the TerclaF3/TerclaR1 primers pair, 89%, and the minimal fungal biomass that could be reliable detected was set at 4.23 µg mycelium/g soil. The spatial distribution of fungal biomass was heterogeneous, and there was not a direct relationship between the quantity of winter soil mycelium and the location/productivity of desert truffles. This protocol could be applied to tracking these species in soil and understand their mycelial dynamics in plantations and wild areas. This research was funded by MCIN/AEI/10.13039/50110001103, grant number PID2020- 115210RB-I00, and FEDER and Fundación Séneca, Agencia de Ciencia y Tecnología of the Region of Murcia (Spain), grant number 20866/PI/18. MDPI https://doi.org/10.1016/j.foreco.2022.120602 https://hdl.handle.net/10459.1/466357 |
| spellingShingle | Design and Validation of qPCR-Specific Primers for Quantification of the Marketed Terfezia claveryi and Terfezia crassiverrucosa in Soil Arenas, Francisco ITS Terfezia claveryi Terfezia crassiverrucosa qPCR Desert truffles rDNA Ascomycetes mycelium |
| status_str | publishedVersion |
| title | Design and Validation of qPCR-Specific Primers for Quantification of the Marketed Terfezia claveryi and Terfezia crassiverrucosa in Soil |
| title_full | Design and Validation of qPCR-Specific Primers for Quantification of the Marketed Terfezia claveryi and Terfezia crassiverrucosa in Soil |
| title_fullStr | Design and Validation of qPCR-Specific Primers for Quantification of the Marketed Terfezia claveryi and Terfezia crassiverrucosa in Soil |
| title_full_unstemmed | Design and Validation of qPCR-Specific Primers for Quantification of the Marketed Terfezia claveryi and Terfezia crassiverrucosa in Soil |
| title_short | Design and Validation of qPCR-Specific Primers for Quantification of the Marketed Terfezia claveryi and Terfezia crassiverrucosa in Soil |
| title_sort | Design and Validation of qPCR-Specific Primers for Quantification of the Marketed Terfezia claveryi and Terfezia crassiverrucosa in Soil |
| topic | ITS Terfezia claveryi Terfezia crassiverrucosa qPCR Desert truffles rDNA Ascomycetes mycelium |
| topic_facet | ITS Terfezia claveryi Terfezia crassiverrucosa qPCR Desert truffles rDNA Ascomycetes mycelium |
| url | https://doi.org/10.1016/j.foreco.2022.120602 https://hdl.handle.net/10459.1/466357 |