Fibroblast migration in 3D is controlled by haptotaxis in a non-muscle myosin II-dependent manner
Cell migration in 3D is a key process in many physiological and pathological processes. Although valuable knowledge has been accumulated through analysis of various 2D models, some of these insights are not directly applicable to migration in 3D. In this study, we have confined biomimetic hydrogels...
| Authors: | ; ; ; ; |
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| Format: | Article |
| Status: | Published version |
| Publication date: | 2015 |
| Country: | España |
| Institution: | Universidad de Zaragoza |
| Repository: | Zaguán. Repositorio Digital de la Universidad de Zaragoza |
| OAI Identifier: | oai:zaguan.unizar.es:32208 |
| Online Access: | http://zaguan.unizar.es/record/32208 |
| Access Level: | Open access |
| Summary: | Cell migration in 3D is a key process in many physiological and pathological processes. Although valuable knowledge has been accumulated through analysis of various 2D models, some of these insights are not directly applicable to migration in 3D. In this study, we have confined biomimetic hydrogels within microfluidic platforms in the presence of a chemoattractant (platelet-derived growth fac- tor-BB). We have characterized the migratory responses of human fibroblasts within them, particularly focusing on the role of non-muscle myosin II. Our results indicate a prominent role for myosin II in the integration of chemo- tactic and haptotactic migratory responses of fibroblasts in 3D confined environments. |
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