High throughput stationary 13C-Metabolic Flux Analysis of 3-hydroxypropionic acid producing Pichia pastoris strains. Supplementary materials of Chapter 5 of the PhD Thesis of Albert Fina Romero
Supplementary files of Chapter 5 of the PhD Thesis of Albert Fina Romero. This chapter describes the fluxome of 3-hydroxypropionic acid producing Pichia pastoris recombinant strains. Glycerol was used as a sole carbon source. A High Throughput platform was used to simultaneously characterize the flu...
| Autores: | ; ; |
|---|---|
| Tipo de documento: | Conjunto de datos |
| Fecha de publicación: | 2022 |
| País: | España |
| Institución: | Universitat Autònoma de Barcelona |
| Repositorio: | Dipòsit Digital de Documents de la UAB |
| Lenguaje: | Inglés |
| OAI Identifier: | oai:ddd.uab.cat:264385 |
| Acceso en línea: | https://ddd.uab.cat/record/264385 https://dx.doi.org/urn:doi:10.5565/ddd.uab.cat/264385 |
| Nivel de acceso: | Acceso abierto |
| Palabra clave: | Fluxomics 13C-Metabolic Flux Analysis (13C-MFA) Pichia pastoris High Throughput Systems Biology Ph 3-Hydroxypropionic acid |
| _version_ | 1878736738206089216 |
|---|---|
| author | Fina Romero, Albert|||0000-0001-7949-0484 |
| author2 | Albiol i Sala, Joan|||0000-0001-5626-429X Ferrer, Pau|||0000-0002-5287-4127 |
| author2_role | author author |
| author_browse | Albiol i Sala, Joan|||0000-0001-5626-429X Ferrer, Pau|||0000-0002-5287-4127 Fina Romero, Albert|||0000-0001-7949-0484 |
| author_facet | Fina Romero, Albert|||0000-0001-7949-0484 Albiol i Sala, Joan|||0000-0001-5626-429X Ferrer, Pau|||0000-0002-5287-4127 |
| author_role | author |
| collection | Dipòsit Digital de Documents de la UAB |
| description | Supplementary files of Chapter 5 of the PhD Thesis of Albert Fina Romero. This chapter describes the fluxome of 3-hydroxypropionic acid producing Pichia pastoris recombinant strains. Glycerol was used as a sole carbon source. A High Throughput platform was used to simultaneously characterize the fluxome of 10 recombinant strains at pH 5 and 6 recombinant strains at pH 3.5. All the experiments were carried in triplicates. To determine the intracellular fluxes, stationary 13C-Metabolic Flux Analysis was used using the data of the isotopologue distribution of the proteinogenic amino acids. The files attached herein contain: the description of how to obtain the models used for this study, the raw data obtained from the mini-bioreactor cultivations, the files to process the raw data, and the results (bioprocess parameters and fluxes from 13C-Metabolic flux analysis and Flux Balance Analysis). |
| eu_rights_str_mv | openAccess |
| format | dataset |
| id | ES_9476f4efd84b1cf34e2e1f45c30d94ce |
| institution | Universitat Autònoma de Barcelona |
| instname_str | Universitat Autònoma de Barcelona |
| language | eng |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:ddd.uab.cat:264385 |
| publishDate | 2022 |
| publishDateSort | 2022 |
| reponame_str | Dipòsit Digital de Documents de la UAB |
| spelling | High throughput stationary 13C-Metabolic Flux Analysis of 3-hydroxypropionic acid producing Pichia pastoris strains. Supplementary materials of Chapter 5 of the PhD Thesis of Albert Fina Romero Fina Romero, Albert|||0000-0001-7949-0484 Albiol i Sala, Joan|||0000-0001-5626-429X Ferrer, Pau|||0000-0002-5287-4127 Fluxomics 13C-Metabolic Flux Analysis (13C-MFA) Pichia pastoris High Throughput Systems Biology Ph 3-Hydroxypropionic acid Supplementary files of Chapter 5 of the PhD Thesis of Albert Fina Romero. This chapter describes the fluxome of 3-hydroxypropionic acid producing Pichia pastoris recombinant strains. Glycerol was used as a sole carbon source. A High Throughput platform was used to simultaneously characterize the fluxome of 10 recombinant strains at pH 5 and 6 recombinant strains at pH 3.5. All the experiments were carried in triplicates. To determine the intracellular fluxes, stationary 13C-Metabolic Flux Analysis was used using the data of the isotopologue distribution of the proteinogenic amino acids. The files attached herein contain: the description of how to obtain the models used for this study, the raw data obtained from the mini-bioreactor cultivations, the files to process the raw data, and the results (bioprocess parameters and fluxes from 13C-Metabolic flux analysis and Flux Balance Analysis). https://ddd.uab.cat/record/264385 https://dx.doi.org/urn:doi:10.5565/ddd.uab.cat/264385 |
| spellingShingle | High throughput stationary 13C-Metabolic Flux Analysis of 3-hydroxypropionic acid producing Pichia pastoris strains. Supplementary materials of Chapter 5 of the PhD Thesis of Albert Fina Romero Fina Romero, Albert|||0000-0001-7949-0484 Fluxomics 13C-Metabolic Flux Analysis (13C-MFA) Pichia pastoris High Throughput Systems Biology Ph 3-Hydroxypropionic acid |
| title | High throughput stationary 13C-Metabolic Flux Analysis of 3-hydroxypropionic acid producing Pichia pastoris strains. Supplementary materials of Chapter 5 of the PhD Thesis of Albert Fina Romero |
| title_full | High throughput stationary 13C-Metabolic Flux Analysis of 3-hydroxypropionic acid producing Pichia pastoris strains. Supplementary materials of Chapter 5 of the PhD Thesis of Albert Fina Romero |
| title_fullStr | High throughput stationary 13C-Metabolic Flux Analysis of 3-hydroxypropionic acid producing Pichia pastoris strains. Supplementary materials of Chapter 5 of the PhD Thesis of Albert Fina Romero |
| title_full_unstemmed | High throughput stationary 13C-Metabolic Flux Analysis of 3-hydroxypropionic acid producing Pichia pastoris strains. Supplementary materials of Chapter 5 of the PhD Thesis of Albert Fina Romero |
| title_short | High throughput stationary 13C-Metabolic Flux Analysis of 3-hydroxypropionic acid producing Pichia pastoris strains. Supplementary materials of Chapter 5 of the PhD Thesis of Albert Fina Romero |
| title_sort | High throughput stationary 13C-Metabolic Flux Analysis of 3-hydroxypropionic acid producing Pichia pastoris strains. Supplementary materials of Chapter 5 of the PhD Thesis of Albert Fina Romero |
| topic | Fluxomics 13C-Metabolic Flux Analysis (13C-MFA) Pichia pastoris High Throughput Systems Biology Ph 3-Hydroxypropionic acid |
| topic_facet | Fluxomics 13C-Metabolic Flux Analysis (13C-MFA) Pichia pastoris High Throughput Systems Biology Ph 3-Hydroxypropionic acid |
| url | https://ddd.uab.cat/record/264385 https://dx.doi.org/urn:doi:10.5565/ddd.uab.cat/264385 |