Facilitation of Insulin Effects by Ranolazine in Astrocytes in Primary Culture.
Ranolazine (Rn) is a drug used to treat persistent chronic coronary ischemia. It has also been shown to have therapeutic benefits on the central nervous system and an anti-diabetic effect by lowering blood glucose levels; however, no effects of Rn on cellular sensitivity to insulin (Ins) have been d...
| Autores: | ; ; ; ; ; ; ; ; ; |
|---|---|
| Tipo de documento: | Artículo |
| Estado: | Published version |
| Fecha de publicación: | 2022 |
| País: | España |
| Institución: | INCLIVA |
| Repositorio: | r-INCLIVA. Repositorio Institucional de Producción Científica de INCLIVA |
| OAI Identifier: | oai:incliva.fundanetsuite.com:p16937 |
| Acceso en línea: | https://incliva.portalinvestigacion.com/publicaciones/16937 |
| Nivel de acceso: | Acceso abierto |
| Palabra clave: | antioxidants astrocytes inflammation insulin ranolazine |
| _version_ | 1878736420109025280 |
|---|---|
| author | Jorda, A |
| author2 | Aldasoro, M Campo-Palacio, I Vila, JM Aldasoro, C Campos-Campos, J Colmena, C Singh, SK Obrdor, E Valles, SL |
| author2_role | author author author author author author author author author |
| author_browse | Aldasoro, C Aldasoro, M Campo-Palacio, I Campos-Campos, J Colmena, C Jorda, A Obrdor, E Singh, SK Valles, SL Vila, JM |
| author_facet | Jorda, A Aldasoro, M Campo-Palacio, I Vila, JM Aldasoro, C Campos-Campos, J Colmena, C Singh, SK Obrdor, E Valles, SL |
| author_role | author |
| collection | r-INCLIVA. Repositorio Institucional de Producción Científica de INCLIVA |
| description | Ranolazine (Rn) is a drug used to treat persistent chronic coronary ischemia. It has also been shown to have therapeutic benefits on the central nervous system and an anti-diabetic effect by lowering blood glucose levels; however, no effects of Rn on cellular sensitivity to insulin (Ins) have been demonstrated yet. The present study aimed to investigate the permissive effects of Rn on the actions of Ins in astrocytes in primary culture. Ins (10(-8) M), Rn (10(-6) M), and Ins + Rn (10(-8) M and 10(-6) M, respectively) were added to astrocytes for 24 h. In comparison to control cells, Rn and/or Ins caused modifications in cell viability and proliferation. Rn increased protein expression of Cu/Zn-SOD and the pro-inflammatory protein COX-2 was upregulated by Ins. On the contrary, no significant changes were found in the protein expression of NF-?B and I?B. The presence of Rn produced an increase in p-ERK protein and a significant decrease in COX-2 protein expression. Furthermore, Rn significantly increased the effects of Ins on the expression of p-AKT, p-eNOS, p-ERK, Mn-SOD, and PPAR-?. In addition, Rn + Ins produced a significant decrease in COX-2 expression. In conclusion, Rn facilitated the effects of insulin on the p-AKT, p-eNOS, p-ERK, Mn-SOD, and PPAR-? signaling pathways, as well as on the anti-inflammatory and antioxidant effects of the hormone. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_8eafebb5ac11140c510b8167f5dfce03 |
| institution | INCLIVA |
| instname_str | INCLIVA |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:incliva.fundanetsuite.com:p16937 |
| publishDate | 2022 |
| publishDateSort | 2022 |
| publisher | MDPI |
| publisherStr | MDPI |
| reponame_str | r-INCLIVA. Repositorio Institucional de Producción Científica de INCLIVA |
| spelling | Facilitation of Insulin Effects by Ranolazine in Astrocytes in Primary Culture. Jorda, A Aldasoro, M Campo-Palacio, I Vila, JM Aldasoro, C Campos-Campos, J Colmena, C Singh, SK Obrdor, E Valles, SL antioxidants astrocytes inflammation insulin ranolazine Ranolazine (Rn) is a drug used to treat persistent chronic coronary ischemia. It has also been shown to have therapeutic benefits on the central nervous system and an anti-diabetic effect by lowering blood glucose levels; however, no effects of Rn on cellular sensitivity to insulin (Ins) have been demonstrated yet. The present study aimed to investigate the permissive effects of Rn on the actions of Ins in astrocytes in primary culture. Ins (10(-8) M), Rn (10(-6) M), and Ins + Rn (10(-8) M and 10(-6) M, respectively) were added to astrocytes for 24 h. In comparison to control cells, Rn and/or Ins caused modifications in cell viability and proliferation. Rn increased protein expression of Cu/Zn-SOD and the pro-inflammatory protein COX-2 was upregulated by Ins. On the contrary, no significant changes were found in the protein expression of NF-?B and I?B. The presence of Rn produced an increase in p-ERK protein and a significant decrease in COX-2 protein expression. Furthermore, Rn significantly increased the effects of Ins on the expression of p-AKT, p-eNOS, p-ERK, Mn-SOD, and PPAR-?. In addition, Rn + Ins produced a significant decrease in COX-2 expression. In conclusion, Rn facilitated the effects of insulin on the p-AKT, p-eNOS, p-ERK, Mn-SOD, and PPAR-? signaling pathways, as well as on the anti-inflammatory and antioxidant effects of the hormone. MDPI https://incliva.portalinvestigacion.com/publicaciones/16937 |
| spellingShingle | Facilitation of Insulin Effects by Ranolazine in Astrocytes in Primary Culture. Jorda, A antioxidants astrocytes inflammation insulin ranolazine |
| status_str | publishedVersion |
| title | Facilitation of Insulin Effects by Ranolazine in Astrocytes in Primary Culture. |
| title_full | Facilitation of Insulin Effects by Ranolazine in Astrocytes in Primary Culture. |
| title_fullStr | Facilitation of Insulin Effects by Ranolazine in Astrocytes in Primary Culture. |
| title_full_unstemmed | Facilitation of Insulin Effects by Ranolazine in Astrocytes in Primary Culture. |
| title_short | Facilitation of Insulin Effects by Ranolazine in Astrocytes in Primary Culture. |
| title_sort | Facilitation of Insulin Effects by Ranolazine in Astrocytes in Primary Culture. |
| topic | antioxidants astrocytes inflammation insulin ranolazine |
| topic_facet | antioxidants astrocytes inflammation insulin ranolazine |
| url | https://incliva.portalinvestigacion.com/publicaciones/16937 |