Incorporation of Elastin to Improve Polycaprolactone-Based Scaffolds for Skeletal Muscle via Electrospinning
Skeletal muscle regeneration is increasingly necessary, which is reflected in the increasing number of studies that are focused on improving the scaffolds used for such regeneration, as well as the incubation protocol. The main objective of this work was to improve the characteristics of polycaprola...
| Autores: | ; ; ; ; |
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| Tipo de documento: | Artículo |
| Estado: | Published version |
| Fecha de publicación: | 2021 |
| País: | España |
| Institución: | Universidad de Sevilla (US) |
| Repositorio: | idUS. Depósito de Investigación de la Universidad de Sevilla |
| OAI Identifier: | oai:idus.us.es:11441/114966 |
| Acceso en línea: | https://hdl.handle.net/11441/114966 https://doi.org/10.3390/polym13091501 |
| Nivel de acceso: | Acceso abierto |
| Palabra clave: | Elastin Electrospinning Scaffolds Skeletal muscle cells |
| _version_ | 1878736416331005952 |
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| author | Pérez-Puyana, Víctor Manuel |
| author2 | Villanueva, Paula Jiménez-Rosado, Mercedes Portilla de Juan, Fernando de la Romero García, Alberto |
| author2_role | author author author author |
| author_browse | Jiménez-Rosado, Mercedes Portilla de Juan, Fernando de la Pérez-Puyana, Víctor Manuel Romero García, Alberto Villanueva, Paula |
| author_facet | Pérez-Puyana, Víctor Manuel Villanueva, Paula Jiménez-Rosado, Mercedes Portilla de Juan, Fernando de la Romero García, Alberto |
| author_role | author |
| collection | idUS. Depósito de Investigación de la Universidad de Sevilla |
| description | Skeletal muscle regeneration is increasingly necessary, which is reflected in the increasing number of studies that are focused on improving the scaffolds used for such regeneration, as well as the incubation protocol. The main objective of this work was to improve the characteristics of polycaprolactone (PCL) scaffolds by incorporating elastin to achieve better cell proliferation and biocompatibility. In addition, two cell incubation protocols (with and without dynamic mechanical stimulation) were evaluated to improve the activity and functionality yields of the regenerated cells. The results indicate that the incorporation of elastin generates aligned and more hydrophilic scaffolds with smaller fiber size. In addition, the mechanical properties of the resulting scaffolds make them adequate for use in both bioreactors and patients. All these characteristics increase the biocompatibility of these systems, generating a better interconnection with the tissue. However, due to the low maturation achieved in biological tests, no differences could be found between the incubation with and without dynamic mechanical stimulation. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_8e9ede67d045011cbd680b4e3114e363 |
| institution | Universidad de Sevilla (US) |
| instname_str | Universidad de Sevilla (US) |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:idus.us.es:11441/114966 |
| publishDate | 2021 |
| publishDateSort | 2021 |
| publisher | Multidisciplinary Digital Publishing Institute (MDPI) |
| publisherStr | Multidisciplinary Digital Publishing Institute (MDPI) |
| reponame_str | idUS. Depósito de Investigación de la Universidad de Sevilla |
| spelling | Incorporation of Elastin to Improve Polycaprolactone-Based Scaffolds for Skeletal Muscle via Electrospinning Pérez-Puyana, Víctor Manuel Villanueva, Paula Jiménez-Rosado, Mercedes Portilla de Juan, Fernando de la Romero García, Alberto Elastin Electrospinning Scaffolds Skeletal muscle cells Skeletal muscle regeneration is increasingly necessary, which is reflected in the increasing number of studies that are focused on improving the scaffolds used for such regeneration, as well as the incubation protocol. The main objective of this work was to improve the characteristics of polycaprolactone (PCL) scaffolds by incorporating elastin to achieve better cell proliferation and biocompatibility. In addition, two cell incubation protocols (with and without dynamic mechanical stimulation) were evaluated to improve the activity and functionality yields of the regenerated cells. The results indicate that the incorporation of elastin generates aligned and more hydrophilic scaffolds with smaller fiber size. In addition, the mechanical properties of the resulting scaffolds make them adequate for use in both bioreactors and patients. All these characteristics increase the biocompatibility of these systems, generating a better interconnection with the tissue. However, due to the low maturation achieved in biological tests, no differences could be found between the incubation with and without dynamic mechanical stimulation. Ministerio de Ciencia e Innovación RTI2018-097100-B-C21 Multidisciplinary Digital Publishing Institute (MDPI) https://hdl.handle.net/11441/114966 https://doi.org/10.3390/polym13091501 |
| spellingShingle | Incorporation of Elastin to Improve Polycaprolactone-Based Scaffolds for Skeletal Muscle via Electrospinning Pérez-Puyana, Víctor Manuel Elastin Electrospinning Scaffolds Skeletal muscle cells |
| status_str | publishedVersion |
| title | Incorporation of Elastin to Improve Polycaprolactone-Based Scaffolds for Skeletal Muscle via Electrospinning |
| title_full | Incorporation of Elastin to Improve Polycaprolactone-Based Scaffolds for Skeletal Muscle via Electrospinning |
| title_fullStr | Incorporation of Elastin to Improve Polycaprolactone-Based Scaffolds for Skeletal Muscle via Electrospinning |
| title_full_unstemmed | Incorporation of Elastin to Improve Polycaprolactone-Based Scaffolds for Skeletal Muscle via Electrospinning |
| title_short | Incorporation of Elastin to Improve Polycaprolactone-Based Scaffolds for Skeletal Muscle via Electrospinning |
| title_sort | Incorporation of Elastin to Improve Polycaprolactone-Based Scaffolds for Skeletal Muscle via Electrospinning |
| topic | Elastin Electrospinning Scaffolds Skeletal muscle cells |
| topic_facet | Elastin Electrospinning Scaffolds Skeletal muscle cells |
| url | https://hdl.handle.net/11441/114966 https://doi.org/10.3390/polym13091501 |