Embryogenic competence of microspores is associated to their ability to form a callosic, osmoprotective subintinal layer
[EN] Microspore embryogenesis is an experimental morphogenic pathway with important applications in basic research and applied plant breeding, but its genetic, cellular, and molecular bases are poorly understood. We applied a multi-disciplinary approach using confocal and electron microscopy, detect...
| Authors: | ; ; ; ; ; |
|---|---|
| Format: | Article |
| Publication date: | 2019 |
| Country: | España |
| Institution: | Universitat Politècnica de València (UPV) |
| Repository: | RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
| Language: | English |
| OAI Identifier: | oai:riunet.upv.es:10251/155497 |
| Online Access: | https://riunet.upv.es/handle/10251/155497 |
| Access Level: | Open access |
| Keyword: | Androgenesis Brassica napus Calcium Cellulose Cell wall Doubled haploids Eggplant Rapeseed GENETICA BIOQUIMICA Y BIOLOGIA MOLECULAR |
| _version_ | 1878736255302238208 |
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| author | Rivas-Sendra, Alba |
| author2 | Corral Martínez, Patricia Camacho-Fernández, Carolina Calabuig-Serna, Antonio Porcel, R.|||0000-0001-8068-741X Seguí-Simarro, Jose M.|||0000-0001-7672-4169 |
| author2_role | author author author author author |
| author_browse | Calabuig-Serna, Antonio Camacho-Fernández, Carolina Corral Martínez, Patricia Porcel, R.|||0000-0001-8068-741X Rivas-Sendra, Alba Seguí-Simarro, Jose M.|||0000-0001-7672-4169 |
| author_facet | Rivas-Sendra, Alba Corral Martínez, Patricia Camacho-Fernández, Carolina Calabuig-Serna, Antonio Porcel, R.|||0000-0001-8068-741X Seguí-Simarro, Jose M.|||0000-0001-7672-4169 |
| author_role | author |
| collection | RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
| description | [EN] Microspore embryogenesis is an experimental morphogenic pathway with important applications in basic research and applied plant breeding, but its genetic, cellular, and molecular bases are poorly understood. We applied a multi-disciplinary approach using confocal and electron microscopy, detection of Ca2+, callose, and cellulose, treatments with caffeine, digitonin, and endosidin7, morphometry, qPCR, osmometry, and viability assays in order to study the dynamics of cell wall formation during embryogenesis induction in a high-response rapeseed (Brassica napus) line and two recalcitrant rapeseed and eggplant (Solanum melongena) lines. Formation of a callose-rich subintinal layer (SL) was common to microspore embryogenesis in the different genotypes. However, this process was directly related to embryogenic response, being greater in high-response genotypes. A link could be established between Ca2+ influx, abnormal callose/cellulose deposition, and the genotype-specific embryogenic competence. Callose deposition in inner walls and SLs are independent processes, regulated by different callose synthases. Viability and control of internal osmolality are also related to SL formation. In summary, we identified one of the causes of recalcitrance to embryogenesis induction: a reduced or absent protective SL. In responding genotypes, SLs are markers for changes in cell fate and serve as osmoprotective barriers to increase viability in imbalanced in vitro environments. Genotype-specific differences relate to different responses against abiotic (heat/osmotic) stresses. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_8bd0b2da970b2ffe88396d38f4a96fef |
| institution | Universitat Politècnica de València (UPV) |
| instname_str | Universitat Politècnica de València (UPV) |
| language | eng |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:riunet.upv.es:10251/155497 |
| publishDate | 2019 |
| publishDateSort | 2019 |
| publisher | Oxford University Press |
| publisherStr | Oxford University Press |
| reponame_str | RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
| spelling | Embryogenic competence of microspores is associated to their ability to form a callosic, osmoprotective subintinal layer Rivas-Sendra, Alba Corral Martínez, Patricia Camacho-Fernández, Carolina Calabuig-Serna, Antonio Porcel, R.|||0000-0001-8068-741X Seguí-Simarro, Jose M.|||0000-0001-7672-4169 Androgenesis Brassica napus Calcium Cellulose Cell wall Doubled haploids Eggplant Rapeseed GENETICA BIOQUIMICA Y BIOLOGIA MOLECULAR [EN] Microspore embryogenesis is an experimental morphogenic pathway with important applications in basic research and applied plant breeding, but its genetic, cellular, and molecular bases are poorly understood. We applied a multi-disciplinary approach using confocal and electron microscopy, detection of Ca2+, callose, and cellulose, treatments with caffeine, digitonin, and endosidin7, morphometry, qPCR, osmometry, and viability assays in order to study the dynamics of cell wall formation during embryogenesis induction in a high-response rapeseed (Brassica napus) line and two recalcitrant rapeseed and eggplant (Solanum melongena) lines. Formation of a callose-rich subintinal layer (SL) was common to microspore embryogenesis in the different genotypes. However, this process was directly related to embryogenic response, being greater in high-response genotypes. A link could be established between Ca2+ influx, abnormal callose/cellulose deposition, and the genotype-specific embryogenic competence. Callose deposition in inner walls and SLs are independent processes, regulated by different callose synthases. Viability and control of internal osmolality are also related to SL formation. In summary, we identified one of the causes of recalcitrance to embryogenesis induction: a reduced or absent protective SL. In responding genotypes, SLs are markers for changes in cell fate and serve as osmoprotective barriers to increase viability in imbalanced in vitro environments. Genotype-specific differences relate to different responses against abiotic (heat/osmotic) stresses. Thanks are due to the Electron Microscopy Service of Universitat Politecnica de Valencia, Marisol Gascon (IBMCP Microscopy Service), Dr Kim Boutilier (WUR, Wageningen) for hosting ARS at her lab, and Dr Samantha Vernhettes (INRA Versailles) for kindly providing us with S4B. This work supported by grants AGL2014-55177-R and AGL2017-88135-R to JMSS from MINECO jointly funded by FEDER. Oxford University Press https://riunet.upv.es/handle/10251/155497 |
| spellingShingle | Embryogenic competence of microspores is associated to their ability to form a callosic, osmoprotective subintinal layer Rivas-Sendra, Alba Androgenesis Brassica napus Calcium Cellulose Cell wall Doubled haploids Eggplant Rapeseed GENETICA BIOQUIMICA Y BIOLOGIA MOLECULAR |
| title | Embryogenic competence of microspores is associated to their ability to form a callosic, osmoprotective subintinal layer |
| title_full | Embryogenic competence of microspores is associated to their ability to form a callosic, osmoprotective subintinal layer |
| title_fullStr | Embryogenic competence of microspores is associated to their ability to form a callosic, osmoprotective subintinal layer |
| title_full_unstemmed | Embryogenic competence of microspores is associated to their ability to form a callosic, osmoprotective subintinal layer |
| title_short | Embryogenic competence of microspores is associated to their ability to form a callosic, osmoprotective subintinal layer |
| title_sort | Embryogenic competence of microspores is associated to their ability to form a callosic, osmoprotective subintinal layer |
| topic | Androgenesis Brassica napus Calcium Cellulose Cell wall Doubled haploids Eggplant Rapeseed GENETICA BIOQUIMICA Y BIOLOGIA MOLECULAR |
| topic_facet | Androgenesis Brassica napus Calcium Cellulose Cell wall Doubled haploids Eggplant Rapeseed GENETICA BIOQUIMICA Y BIOLOGIA MOLECULAR |
| url | https://riunet.upv.es/handle/10251/155497 |