Biofunctionalization with a TGF -1 inhibitor peptide in the osseointegration of synthetic bone grafts: an in vivo study in Beagle dogs
Objectives: The aim of this research was to determine the osseointegration of two presentations of biphasic calcium phosphate (BCP) biomaterial¿one untreated and another submitted to biofunctionalization with a TGF- 1 inhibitor peptide, P144, on dental alveolus. Materials and Methods: A synthetic bo...
| Autores: | ; ; ; ; ; |
|---|---|
| Tipo de documento: | Artículo |
| Estado: | Published version |
| Fecha de publicación: | 2019 |
| País: | España |
| Institución: | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| Repositorio: | Recercat. Dipósit de la Recerca de Catalunya |
| OAI Identifier: | oai:recercat.cat:2445/185633 |
| Acceso en línea: | https://hdl.handle.net/2445/185633 |
| Nivel de acceso: | Acceso abierto |
| Palabra clave: | Teixit ossi Empelts ossis Cirurgia dental Experimentació animal Pèptids Bone Bone grafting Dental surgery Animal experimentation Peptides |
| _version_ | 1878736158733631488 |
|---|---|
| author | Cirera, Andrea |
| author2 | Manzanares Céspedes, María Cristina Sevilla, Pablo Ortiz Hernández, Mónica Galindo Moreno, Pablo Gil, Javier |
| author2_role | author author author author author |
| author_browse | Cirera, Andrea Galindo Moreno, Pablo Gil, Javier Manzanares Céspedes, María Cristina Ortiz Hernández, Mónica Sevilla, Pablo |
| author_facet | Cirera, Andrea Manzanares Céspedes, María Cristina Sevilla, Pablo Ortiz Hernández, Mónica Galindo Moreno, Pablo Gil, Javier |
| author_role | author |
| collection | Recercat. Dipósit de la Recerca de Catalunya |
| description | Objectives: The aim of this research was to determine the osseointegration of two presentations of biphasic calcium phosphate (BCP) biomaterial¿one untreated and another submitted to biofunctionalization with a TGF- 1 inhibitor peptide, P144, on dental alveolus. Materials and Methods: A synthetic bone graft was used, namely, (i) Maxresorb® (Botiss Klockner) (n = 12), and (ii) Maxresorb® (Botiss Klockner) biofunctionalized with P144 peptide (n = 12). Both bone grafts were implanted in the two hemimandibles of six beagle dogs in the same surgical time, immediately after tooth extraction. Two dogs were sacrificed 2, 4, and 8 weeks post implant insertion, respectively. The samples were submitted to histomorphometrical and histological analyses. For each sample, we quantified the new bone growth and the new bone formed around the biomaterial's granules. After optical microscopic histological evaluation, selected samples were studied using backscattered scanning electron microscopy (BS-SEM). Results: The biofunctionalization of the biomaterial's granules maintains a stable membranous bone formation throughout the experiment timeline, benefitting from the constant presence of vascular structures in the alveolar space, in a more active manner that in the control samples. Better results in the experimental groups were proven both by quantitative and qualitative analysis. Conclusions: Synthetic bone graft biofunctionalization results in slightly better quantitative parameters of the implant's osseointegration. The qualitative histological and ultramicroscopic analysis shows that biofunctionalization may shorten the healing period of dental biomaterials. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_8a0dd6c1faed03d18a27bf72f7b42962 |
| institution | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| instname_str | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:recercat.cat:2445/185633 |
| publishDate | 2019 |
| publishDateSort | 2019 |
| publisher | MDPI |
| publisherStr | MDPI |
| reponame_str | Recercat. Dipósit de la Recerca de Catalunya |
| spelling | Biofunctionalization with a TGF -1 inhibitor peptide in the osseointegration of synthetic bone grafts: an in vivo study in Beagle dogs Cirera, Andrea Manzanares Céspedes, María Cristina Sevilla, Pablo Ortiz Hernández, Mónica Galindo Moreno, Pablo Gil, Javier Teixit ossi Empelts ossis Cirurgia dental Experimentació animal Pèptids Bone Bone grafting Dental surgery Animal experimentation Peptides Objectives: The aim of this research was to determine the osseointegration of two presentations of biphasic calcium phosphate (BCP) biomaterial¿one untreated and another submitted to biofunctionalization with a TGF- 1 inhibitor peptide, P144, on dental alveolus. Materials and Methods: A synthetic bone graft was used, namely, (i) Maxresorb® (Botiss Klockner) (n = 12), and (ii) Maxresorb® (Botiss Klockner) biofunctionalized with P144 peptide (n = 12). Both bone grafts were implanted in the two hemimandibles of six beagle dogs in the same surgical time, immediately after tooth extraction. Two dogs were sacrificed 2, 4, and 8 weeks post implant insertion, respectively. The samples were submitted to histomorphometrical and histological analyses. For each sample, we quantified the new bone growth and the new bone formed around the biomaterial's granules. After optical microscopic histological evaluation, selected samples were studied using backscattered scanning electron microscopy (BS-SEM). Results: The biofunctionalization of the biomaterial's granules maintains a stable membranous bone formation throughout the experiment timeline, benefitting from the constant presence of vascular structures in the alveolar space, in a more active manner that in the control samples. Better results in the experimental groups were proven both by quantitative and qualitative analysis. Conclusions: Synthetic bone graft biofunctionalization results in slightly better quantitative parameters of the implant's osseointegration. The qualitative histological and ultramicroscopic analysis shows that biofunctionalization may shorten the healing period of dental biomaterials. MDPI https://hdl.handle.net/2445/185633 |
| spellingShingle | Biofunctionalization with a TGF -1 inhibitor peptide in the osseointegration of synthetic bone grafts: an in vivo study in Beagle dogs Cirera, Andrea Teixit ossi Empelts ossis Cirurgia dental Experimentació animal Pèptids Bone Bone grafting Dental surgery Animal experimentation Peptides |
| status_str | publishedVersion |
| title | Biofunctionalization with a TGF -1 inhibitor peptide in the osseointegration of synthetic bone grafts: an in vivo study in Beagle dogs |
| title_full | Biofunctionalization with a TGF -1 inhibitor peptide in the osseointegration of synthetic bone grafts: an in vivo study in Beagle dogs |
| title_fullStr | Biofunctionalization with a TGF -1 inhibitor peptide in the osseointegration of synthetic bone grafts: an in vivo study in Beagle dogs |
| title_full_unstemmed | Biofunctionalization with a TGF -1 inhibitor peptide in the osseointegration of synthetic bone grafts: an in vivo study in Beagle dogs |
| title_short | Biofunctionalization with a TGF -1 inhibitor peptide in the osseointegration of synthetic bone grafts: an in vivo study in Beagle dogs |
| title_sort | Biofunctionalization with a TGF -1 inhibitor peptide in the osseointegration of synthetic bone grafts: an in vivo study in Beagle dogs |
| topic | Teixit ossi Empelts ossis Cirurgia dental Experimentació animal Pèptids Bone Bone grafting Dental surgery Animal experimentation Peptides |
| topic_facet | Teixit ossi Empelts ossis Cirurgia dental Experimentació animal Pèptids Bone Bone grafting Dental surgery Animal experimentation Peptides |
| url | https://hdl.handle.net/2445/185633 |