Besnoitia besnoiti lytic cycle in vitro and differences in invasion and intracellular proliferation among isolates
© 2016 Frey et al. Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit t...
| Authors: | ; ; ; ; ; ; ; ; ; ; ; ; |
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| Format: | Article |
| Status: | Published version |
| Publication date: | 2016 |
| Country: | España |
| Institution: | Universidad Rey Juan Carlos |
| Repository: | BULERIA. Repositorio Institucional de la Universidad de León |
| OAI Identifier: | oai:buleria.unileon.es:10612/19864 |
| Online Access: | https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-016-1405-9 https://hdl.handle.net/10612/19864 |
| Access Level: | Open access |
| Keyword: | Veterinaria Besnoitia besnoiti Besnoitia tarandi Lytic cycle In vitro model Isolates Invasion Proliferation 3109 Ciencias Veterinarias 2401.12 Parasitología Animal |
| _version_ | 1878736158591025152 |
|---|---|
| author | Frey, Caroline F. |
| author2 | Regidor Cerrillo, Javier Marreros, Nelson García Lunar, Paula Gutiérrez Expósito, Daniel Schares, Gereon Dubey, Jitender P. Gentile, Arcangelo Jacquiet, Philippe Shkap, Varda Cortes, Helder Ortega Mora, Luis Miguel Álvarez García, Gema |
| author2_role | author author author author author author author author author author author author |
| author_browse | Cortes, Helder Dubey, Jitender P. Frey, Caroline F. García Lunar, Paula Gentile, Arcangelo Gutiérrez Expósito, Daniel Jacquiet, Philippe Marreros, Nelson Ortega Mora, Luis Miguel Regidor Cerrillo, Javier Schares, Gereon Shkap, Varda Álvarez García, Gema |
| author_facet | Frey, Caroline F. Regidor Cerrillo, Javier Marreros, Nelson García Lunar, Paula Gutiérrez Expósito, Daniel Schares, Gereon Dubey, Jitender P. Gentile, Arcangelo Jacquiet, Philippe Shkap, Varda Cortes, Helder Ortega Mora, Luis Miguel Álvarez García, Gema |
| author_role | author |
| collection | BULERIA. Repositorio Institucional de la Universidad de León |
| description | © 2016 Frey et al. Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_8a0a0acdd7effc7a8a560309a72d00a6 |
| institution | Universidad Rey Juan Carlos |
| instname_str | Universidad Rey Juan Carlos |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:buleria.unileon.es:10612/19864 |
| publishDate | 2016 |
| publishDateSort | 2016 |
| publisher | BMC |
| publisherStr | BMC |
| reponame_str | BULERIA. Repositorio Institucional de la Universidad de León |
| spelling | Besnoitia besnoiti lytic cycle in vitro and differences in invasion and intracellular proliferation among isolates Frey, Caroline F. Regidor Cerrillo, Javier Marreros, Nelson García Lunar, Paula Gutiérrez Expósito, Daniel Schares, Gereon Dubey, Jitender P. Gentile, Arcangelo Jacquiet, Philippe Shkap, Varda Cortes, Helder Ortega Mora, Luis Miguel Álvarez García, Gema Veterinaria Besnoitia besnoiti Besnoitia tarandi Lytic cycle In vitro model Isolates Invasion Proliferation 3109 Ciencias Veterinarias 2401.12 Parasitología Animal © 2016 Frey et al. Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. [EN]Background: Bovine besnoitiosis, caused by the protozoan Besnoitia besnoiti, reduces productivity and fertility of affected herds. Besnoitiosis continues to expand in Europe and no effective control tools are currently available. Experimental models are urgently needed. Herein, we describe for the first time the kinetics of standardised in vitro models for the B. besnoiti lytic cycle. This will aid to study the pathogenesis of the disease, in the screening for vaccine targets and drugs potentially useful for the treatment of besnoitiosis. Methods: We compared invasion and proliferation of one B. tarandi (from Finland) and seven B. besnoiti isolates (Bb-Spain1, Bb-Spain2, Bb-Israel, Bb-Evora03, Bb-Ger1, Bb-France, Bb-Italy2) in MARC-145 cell culture. Host cell invasion was studied at 4, 6, 8 and 24 h post infection (hpi), and proliferation characteristics were compared at 24, 48, 72, 96, 120, and 144 hpi. Results: In Besnoitia spp., the key parameters that determine the sequential adhesion-invasion, proliferation and egress steps are clearly distinct from those in the related apicomplexans Toxoplasma gondii and Neospora caninum. Besnoitia spp. host cell invasion is a rather slow process, since only 50 % of parasites were found intracellular after 3-6 h of exposure to host cells, and invasion still took place after 24 h. Invasion efficacy was significantly higher for Bb-France, Bb-Evora03 and Bb-Israel. In addition, the time span for endodyogeny to take place was as long as 18-35 h. Bb-Israel and B. tarandi isolates were most prolific, as determined by the tachyzoite yield at 72 hpi. The total tachyzoite yield could not be predicted neither by invasion-related parameters (velocity and half time invasion) nor by proliferation parameters (lag phase and doubling time (dT)). The lytic cycle of Besnoitia was asynchronous as evidenced by the presence of three different plaque-forming tachyzoite categories (lysis plaques, large and small parasitophorous vacuoles). Conclusions: This study provides first insights into the lytic cycle of B. besnoiti isolates and a standardised in vitro model that allows screening of drug candidates for the treatment of besnoitiosis. SI This study was funded by the Spanish Ministry of Economy and Competitiveness (AGL2013-46442-R) and CYTED (Thematic Network 113RT0469 Protozoovac). CFF and NM are supported by the Swiss National Science Foundation (grants no. PBBEP3_141435 and PBBEP3_139398, respectively). We gratefully acknowledge Vanessa Navarro for her excellent technical assistance, and Andrew Hemphill for critical reading of the manuscript. BMC https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-016-1405-9 https://hdl.handle.net/10612/19864 |
| spellingShingle | Besnoitia besnoiti lytic cycle in vitro and differences in invasion and intracellular proliferation among isolates Frey, Caroline F. Veterinaria Besnoitia besnoiti Besnoitia tarandi Lytic cycle In vitro model Isolates Invasion Proliferation 3109 Ciencias Veterinarias 2401.12 Parasitología Animal |
| status_str | publishedVersion |
| title | Besnoitia besnoiti lytic cycle in vitro and differences in invasion and intracellular proliferation among isolates |
| title_full | Besnoitia besnoiti lytic cycle in vitro and differences in invasion and intracellular proliferation among isolates |
| title_fullStr | Besnoitia besnoiti lytic cycle in vitro and differences in invasion and intracellular proliferation among isolates |
| title_full_unstemmed | Besnoitia besnoiti lytic cycle in vitro and differences in invasion and intracellular proliferation among isolates |
| title_short | Besnoitia besnoiti lytic cycle in vitro and differences in invasion and intracellular proliferation among isolates |
| title_sort | Besnoitia besnoiti lytic cycle in vitro and differences in invasion and intracellular proliferation among isolates |
| topic | Veterinaria Besnoitia besnoiti Besnoitia tarandi Lytic cycle In vitro model Isolates Invasion Proliferation 3109 Ciencias Veterinarias 2401.12 Parasitología Animal |
| topic_facet | Veterinaria Besnoitia besnoiti Besnoitia tarandi Lytic cycle In vitro model Isolates Invasion Proliferation 3109 Ciencias Veterinarias 2401.12 Parasitología Animal |
| url | https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-016-1405-9 https://hdl.handle.net/10612/19864 |