Sinorhizobium meliloti DnaJ Is Required for Surface Motility, Stress Tolerance, and for Efficient Nodulation and Symbiotic Nitrogen Fixation
Bacterial surface motility is a complex microbial trait that contributes to host colonization. However, the knowledge about regulatory mechanisms that control surface translocation in rhizobia and their role in the establishment of symbiosis with legumes is still limited. Recently, 2-tridecanone (2-...
| Authors: | ; ; ; ; ; ; ; ; |
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| Format: | Article |
| Status: | Published version |
| Publication date: | 2023 |
| Country: | España |
| Institution: | Universidad de Sevilla (US) |
| Repository: | idUS. Depósito de Investigación de la Universidad de Sevilla |
| OAI Identifier: | oai:idus.us.es:11441/148099 |
| Online Access: | https://hdl.handle.net/11441/148099 https://doi.org/10.3390/ijms24065848 |
| Access Level: | Open access |
| Keyword: | Chaperone Flagella Nitrogen fixation Nodulation Plant colonization Rhizobium Stress tolerance Surface motility |
| _version_ | 1878735990053404672 |
|---|---|
| author | Brito Santana, Paula |
| author2 | Duque Pedraza, Julián J. Bernabéu Roda, Lydia M. Carvia-Hermoso, Cristina Cuéllar, Virginia Fuentes Romero, Francisco Acosta Jurado, Sebastián Vinardell González, José María Soto, María J. |
| author2_role | author author author author author author author author |
| author_browse | Acosta Jurado, Sebastián Bernabéu Roda, Lydia M. Brito Santana, Paula Carvia-Hermoso, Cristina Cuéllar, Virginia Duque Pedraza, Julián J. Fuentes Romero, Francisco Soto, María J. Vinardell González, José María |
| author_facet | Brito Santana, Paula Duque Pedraza, Julián J. Bernabéu Roda, Lydia M. Carvia-Hermoso, Cristina Cuéllar, Virginia Fuentes Romero, Francisco Acosta Jurado, Sebastián Vinardell González, José María Soto, María J. |
| author_role | author |
| collection | idUS. Depósito de Investigación de la Universidad de Sevilla |
| description | Bacterial surface motility is a complex microbial trait that contributes to host colonization. However, the knowledge about regulatory mechanisms that control surface translocation in rhizobia and their role in the establishment of symbiosis with legumes is still limited. Recently, 2-tridecanone (2-TDC) was identified as an infochemical in bacteria that hampers microbial colonization of plants. In the alfalfa symbiont Sinorhizobium meliloti, 2-TDC promotes a mode of surface motility that is mostly independent of flagella. To understand the mechanism of action of 2-TDC in S. meliloti and unveil genes putatively involved in plant colonization, Tn5 transposants derived from a flagellaless strain that were impaired in 2-TDC-induced surface spreading were isolated and genetically characterized. In one of the mutants, the gene coding for the chaperone DnaJ was inactivated. Characterization of this transposant and newly obtained flagella-minus and flagella-plus dnaJ deletion mutants revealed that DnaJ is essential for surface translocation, while it plays a minor role in swimming motility. DnaJ loss-of-function reduces salt and oxidative stress tolerance in S. meliloti and hinders the establishment of efficient symbiosis by affecting nodule formation efficiency, cellular infection, and nitrogen fixation. Intriguingly, the lack of DnaJ causes more severe defects in a flagellaless background. This work highlights the role of DnaJ in the free-living and symbiotic lifestyles of S. meliloti. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_86d2ddfd4ab3de9c88b9456c3daca3d9 |
| institution | Universidad de Sevilla (US) |
| instname_str | Universidad de Sevilla (US) |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:idus.us.es:11441/148099 |
| publishDate | 2023 |
| publishDateSort | 2023 |
| publisher | Multidisciplinary Digital Publishing Institute (MDPI) |
| publisherStr | Multidisciplinary Digital Publishing Institute (MDPI) |
| reponame_str | idUS. Depósito de Investigación de la Universidad de Sevilla |
| spelling | Sinorhizobium meliloti DnaJ Is Required for Surface Motility, Stress Tolerance, and for Efficient Nodulation and Symbiotic Nitrogen Fixation Brito Santana, Paula Duque Pedraza, Julián J. Bernabéu Roda, Lydia M. Carvia-Hermoso, Cristina Cuéllar, Virginia Fuentes Romero, Francisco Acosta Jurado, Sebastián Vinardell González, José María Soto, María J. Chaperone Flagella Nitrogen fixation Nodulation Plant colonization Rhizobium Stress tolerance Surface motility Bacterial surface motility is a complex microbial trait that contributes to host colonization. However, the knowledge about regulatory mechanisms that control surface translocation in rhizobia and their role in the establishment of symbiosis with legumes is still limited. Recently, 2-tridecanone (2-TDC) was identified as an infochemical in bacteria that hampers microbial colonization of plants. In the alfalfa symbiont Sinorhizobium meliloti, 2-TDC promotes a mode of surface motility that is mostly independent of flagella. To understand the mechanism of action of 2-TDC in S. meliloti and unveil genes putatively involved in plant colonization, Tn5 transposants derived from a flagellaless strain that were impaired in 2-TDC-induced surface spreading were isolated and genetically characterized. In one of the mutants, the gene coding for the chaperone DnaJ was inactivated. Characterization of this transposant and newly obtained flagella-minus and flagella-plus dnaJ deletion mutants revealed that DnaJ is essential for surface translocation, while it plays a minor role in swimming motility. DnaJ loss-of-function reduces salt and oxidative stress tolerance in S. meliloti and hinders the establishment of efficient symbiosis by affecting nodule formation efficiency, cellular infection, and nitrogen fixation. Intriguingly, the lack of DnaJ causes more severe defects in a flagellaless background. This work highlights the role of DnaJ in the free-living and symbiotic lifestyles of S. meliloti. Ministerio de Ciencia e Innovación PGC2018-096477-B-I00, PID2019-107634R, PID2021-123540NB-I00 Multidisciplinary Digital Publishing Institute (MDPI) https://hdl.handle.net/11441/148099 https://doi.org/10.3390/ijms24065848 |
| spellingShingle | Sinorhizobium meliloti DnaJ Is Required for Surface Motility, Stress Tolerance, and for Efficient Nodulation and Symbiotic Nitrogen Fixation Brito Santana, Paula Chaperone Flagella Nitrogen fixation Nodulation Plant colonization Rhizobium Stress tolerance Surface motility |
| status_str | publishedVersion |
| title | Sinorhizobium meliloti DnaJ Is Required for Surface Motility, Stress Tolerance, and for Efficient Nodulation and Symbiotic Nitrogen Fixation |
| title_full | Sinorhizobium meliloti DnaJ Is Required for Surface Motility, Stress Tolerance, and for Efficient Nodulation and Symbiotic Nitrogen Fixation |
| title_fullStr | Sinorhizobium meliloti DnaJ Is Required for Surface Motility, Stress Tolerance, and for Efficient Nodulation and Symbiotic Nitrogen Fixation |
| title_full_unstemmed | Sinorhizobium meliloti DnaJ Is Required for Surface Motility, Stress Tolerance, and for Efficient Nodulation and Symbiotic Nitrogen Fixation |
| title_short | Sinorhizobium meliloti DnaJ Is Required for Surface Motility, Stress Tolerance, and for Efficient Nodulation and Symbiotic Nitrogen Fixation |
| title_sort | Sinorhizobium meliloti DnaJ Is Required for Surface Motility, Stress Tolerance, and for Efficient Nodulation and Symbiotic Nitrogen Fixation |
| topic | Chaperone Flagella Nitrogen fixation Nodulation Plant colonization Rhizobium Stress tolerance Surface motility |
| topic_facet | Chaperone Flagella Nitrogen fixation Nodulation Plant colonization Rhizobium Stress tolerance Surface motility |
| url | https://hdl.handle.net/11441/148099 https://doi.org/10.3390/ijms24065848 |