Detection of Brucella in Dermacentor Ticks of Wild Boar with Brucellosis
Brucellosis is a sanitary and economically relevant disease affecting humans, livestock, and wildlife. Ticks have been suggested as vectors, long-term carriers, and amplifiers of Brucella. In this study, ticks from wildlife ungulate hosts living in hunting reserves of a central region of Spain were...
| Authors: | ; ; ; ; ; ; ; |
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| Format: | Article |
| Publication date: | 2024 |
| Country: | España |
| Institution: | Universidad Complutense de Madrid (UCM) |
| Repository: | Docta Complutense |
| Language: | English |
| OAI Identifier: | oai:docta.ucm.es:20.500.14352/102528 |
| Online Access: | https://hdl.handle.net/20.500.14352/102528 |
| Access Level: | Open access |
| Keyword: | 636.09 Veterinaria 3109 Ciencias Veterinarias |
| _version_ | 1878735899238334464 |
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| author | Rebollada Merino, Agustín Miguel |
| author2 | Martínez Alares, Irene Duque, Clara García-Seco Romero, María Teresa Escacena, Cristina Domínguez Rodríguez, Lucas José Rodríguez Bertos, Antonio Manuel García Benzaquén, Nerea |
| author2_role | author author author author author author author |
| author_browse | Domínguez Rodríguez, Lucas José Duque, Clara Escacena, Cristina García Benzaquén, Nerea García-Seco Romero, María Teresa Martínez Alares, Irene Rebollada Merino, Agustín Miguel Rodríguez Bertos, Antonio Manuel |
| author_facet | Rebollada Merino, Agustín Miguel Martínez Alares, Irene Duque, Clara García-Seco Romero, María Teresa Escacena, Cristina Domínguez Rodríguez, Lucas José Rodríguez Bertos, Antonio Manuel García Benzaquén, Nerea |
| author_role | author |
| collection | Docta Complutense |
| description | Brucellosis is a sanitary and economically relevant disease affecting humans, livestock, and wildlife. Ticks have been suggested as vectors, long-term carriers, and amplifiers of Brucella. In this study, ticks from wildlife ungulate hosts living in hunting reserves of a central region of Spain were collected during a 6-year period, pooled, and screened for Brucella spp. by PCR. Aiming to correlate Brucella spp. DNA presence in ticks with Brucella spp. infections in wildlife ungulate hosts, liver samples from deceased wildlife ungulates coming from the hunting reserves showing a positive result for Brucella in ticks were tested using a commercial ELISA. In total, 229 tick pools from wild boar (Sus scrofa, n = 176; 76.8%, 95% CI 70.9%–81.8%), red deer (Cervus elaphus, n = 40; 17.4%, 95% CI 13.1%–22.9%), mouflon (Ovis orientalis musimon, n = 7; 3.06%, 95% CI 1.49%–6.17%), and fallow deer (Dama dama, n = 6; 2.62%, 95% CI 1.21%–5.60%) were analyzed. PCR results showed that 3.93% (95% CI 2.08%–7.30%) tick pools (9/229) from 16.6% hunting reserves (7/41) screened yielded a positive PCR result for Brucella. All positive ticks were Dermacentor (Dermacentor marginatus or Dermacentor reticulatus) collected from wild boar. Ticks collected from wild boars were positive to Brucella in a relative percentage of 5.10% (95% CI = 1.61–11.4) in 2018 and of 7.59% (95% CI = 2.79–15.6) in 2021 (6-year prevalence of 5.17%, 9/176). ELISA showed positive results in three wild boars coming from two out of seven hunting reserves (28.5%) with a positive PCR for Brucella in ticks. To conclude, Brucella spp. DNA can be detected in Dermacentor ticks parasitizing wild boars living in hunting reserves harboring Brucella spp.-seropositive wild boars. This study provides evidence that the contribution of arthropod vectors should be considered in the epidemiology of brucellosis in wildlife. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_8534cfa45ca660821f7cc82d4b261663 |
| institution | Universidad Complutense de Madrid (UCM) |
| instname_str | Universidad Complutense de Madrid (UCM) |
| language | eng |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:docta.ucm.es:20.500.14352/102528 |
| publishDate | 2024 |
| publishDateSort | 2024 |
| publisher | Hindawi |
| publisherStr | Hindawi |
| reponame_str | Docta Complutense |
| spelling | Detection of Brucella in Dermacentor Ticks of Wild Boar with Brucellosis Rebollada Merino, Agustín Miguel Martínez Alares, Irene Duque, Clara García-Seco Romero, María Teresa Escacena, Cristina Domínguez Rodríguez, Lucas José Rodríguez Bertos, Antonio Manuel García Benzaquén, Nerea 636.09 Veterinaria 3109 Ciencias Veterinarias Brucellosis is a sanitary and economically relevant disease affecting humans, livestock, and wildlife. Ticks have been suggested as vectors, long-term carriers, and amplifiers of Brucella. In this study, ticks from wildlife ungulate hosts living in hunting reserves of a central region of Spain were collected during a 6-year period, pooled, and screened for Brucella spp. by PCR. Aiming to correlate Brucella spp. DNA presence in ticks with Brucella spp. infections in wildlife ungulate hosts, liver samples from deceased wildlife ungulates coming from the hunting reserves showing a positive result for Brucella in ticks were tested using a commercial ELISA. In total, 229 tick pools from wild boar (Sus scrofa, n = 176; 76.8%, 95% CI 70.9%–81.8%), red deer (Cervus elaphus, n = 40; 17.4%, 95% CI 13.1%–22.9%), mouflon (Ovis orientalis musimon, n = 7; 3.06%, 95% CI 1.49%–6.17%), and fallow deer (Dama dama, n = 6; 2.62%, 95% CI 1.21%–5.60%) were analyzed. PCR results showed that 3.93% (95% CI 2.08%–7.30%) tick pools (9/229) from 16.6% hunting reserves (7/41) screened yielded a positive PCR result for Brucella. All positive ticks were Dermacentor (Dermacentor marginatus or Dermacentor reticulatus) collected from wild boar. Ticks collected from wild boars were positive to Brucella in a relative percentage of 5.10% (95% CI = 1.61–11.4) in 2018 and of 7.59% (95% CI = 2.79–15.6) in 2021 (6-year prevalence of 5.17%, 9/176). ELISA showed positive results in three wild boars coming from two out of seven hunting reserves (28.5%) with a positive PCR for Brucella in ticks. To conclude, Brucella spp. DNA can be detected in Dermacentor ticks parasitizing wild boars living in hunting reserves harboring Brucella spp.-seropositive wild boars. This study provides evidence that the contribution of arthropod vectors should be considered in the epidemiology of brucellosis in wildlife. Hindawi https://hdl.handle.net/20.500.14352/102528 |
| spellingShingle | Detection of Brucella in Dermacentor Ticks of Wild Boar with Brucellosis Rebollada Merino, Agustín Miguel 636.09 Veterinaria 3109 Ciencias Veterinarias |
| title | Detection of Brucella in Dermacentor Ticks of Wild Boar with Brucellosis |
| title_full | Detection of Brucella in Dermacentor Ticks of Wild Boar with Brucellosis |
| title_fullStr | Detection of Brucella in Dermacentor Ticks of Wild Boar with Brucellosis |
| title_full_unstemmed | Detection of Brucella in Dermacentor Ticks of Wild Boar with Brucellosis |
| title_short | Detection of Brucella in Dermacentor Ticks of Wild Boar with Brucellosis |
| title_sort | Detection of Brucella in Dermacentor Ticks of Wild Boar with Brucellosis |
| topic | 636.09 Veterinaria 3109 Ciencias Veterinarias |
| topic_facet | 636.09 Veterinaria 3109 Ciencias Veterinarias |
| url | https://hdl.handle.net/20.500.14352/102528 |