Insulin controls triacylglycerol synthesis through control of glycerol metabolism and despite increased lipogenesis
Under normoxic conditions, adipocytes in primary culture convert huge amounts of glucose to lactate and glycerol. This 'wasting' of glucose may help to diminish hyperglycemia. Given the importance of insulin in the metabolism, we have studied how it affects adipocyte response to varying glucose leve...
| Autores: | ; ; ; ; ; ; |
|---|---|
| Tipo de documento: | Artículo |
| Estado: | Published version |
| Fecha de publicación: | 2019 |
| País: | España |
| Institución: | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| Repositorio: | Recercat. Dipósit de la Recerca de Catalunya |
| OAI Identifier: | oai:recercat.cat:2445/129707 |
| Acceso en línea: | https://hdl.handle.net/2445/129707 |
| Nivel de acceso: | Acceso abierto |
| Palabra clave: | Glucosa Lípids Insulina Glucose Lipids Insulin |
| _version_ | 1878735588529537024 |
|---|---|
| author | Ho-Palma, A. C. |
| author2 | Toro Espinoza, Pablo Rotondo, Floriana Romero Romero, María del Mar Alemany, Marià, 1946- Remesar Betlloch, Xavier Fernández López, José Antonio |
| author2_role | author author author author author author |
| author_browse | Alemany, Marià, 1946- Fernández López, José Antonio Ho-Palma, A. C. Remesar Betlloch, Xavier Romero Romero, María del Mar Rotondo, Floriana Toro Espinoza, Pablo |
| author_facet | Ho-Palma, A. C. Toro Espinoza, Pablo Rotondo, Floriana Romero Romero, María del Mar Alemany, Marià, 1946- Remesar Betlloch, Xavier Fernández López, José Antonio |
| author_role | author |
| collection | Recercat. Dipósit de la Recerca de Catalunya |
| description | Under normoxic conditions, adipocytes in primary culture convert huge amounts of glucose to lactate and glycerol. This 'wasting' of glucose may help to diminish hyperglycemia. Given the importance of insulin in the metabolism, we have studied how it affects adipocyte response to varying glucose levels, and whether the high basal conversion of glucose to 3-carbon fragments is affected by insulin. Rat fat cells were incubated for 24 h in the presence or absence of 175 nM insulin and 3.5, 7, or 14 mM glucose; half of the wells contained 14C-glucose. We analyzed glucose label fate, medium metabolites, and the expression of key genes controlling glucose and lipid metabolism. Insulin increased both glucose uptake and the flow of carbon through glycolysis and lipogenesis. Lactate excretion was related to medium glucose levels, which agrees with the purported role of disposing excess (circulating) glucose. When medium glucose was low, most basal glycerol came from lipolysis, but when glucose was high, release of glycerol via breakup of glycerol-3P was predominant. Although insulin promotes lipogenesis, it also limited the synthesis of glycerol-3P from glucose and its incorporation into acyl-glycerols. We assume that this is a mechanism of adipose tissue defense to avoid crippling fat accumulation which has not yet been described. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_7fbccdd669b5b2e9f48d4c3c6db596a7 |
| institution | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| instname_str | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:recercat.cat:2445/129707 |
| publishDate | 2019 |
| publishDateSort | 2019 |
| publisher | MDPI |
| publisherStr | MDPI |
| reponame_str | Recercat. Dipósit de la Recerca de Catalunya |
| spelling | Insulin controls triacylglycerol synthesis through control of glycerol metabolism and despite increased lipogenesis Ho-Palma, A. C. Toro Espinoza, Pablo Rotondo, Floriana Romero Romero, María del Mar Alemany, Marià, 1946- Remesar Betlloch, Xavier Fernández López, José Antonio Glucosa Lípids Insulina Glucose Lipids Insulin Under normoxic conditions, adipocytes in primary culture convert huge amounts of glucose to lactate and glycerol. This 'wasting' of glucose may help to diminish hyperglycemia. Given the importance of insulin in the metabolism, we have studied how it affects adipocyte response to varying glucose levels, and whether the high basal conversion of glucose to 3-carbon fragments is affected by insulin. Rat fat cells were incubated for 24 h in the presence or absence of 175 nM insulin and 3.5, 7, or 14 mM glucose; half of the wells contained 14C-glucose. We analyzed glucose label fate, medium metabolites, and the expression of key genes controlling glucose and lipid metabolism. Insulin increased both glucose uptake and the flow of carbon through glycolysis and lipogenesis. Lactate excretion was related to medium glucose levels, which agrees with the purported role of disposing excess (circulating) glucose. When medium glucose was low, most basal glycerol came from lipolysis, but when glucose was high, release of glycerol via breakup of glycerol-3P was predominant. Although insulin promotes lipogenesis, it also limited the synthesis of glycerol-3P from glucose and its incorporation into acyl-glycerols. We assume that this is a mechanism of adipose tissue defense to avoid crippling fat accumulation which has not yet been described. MDPI https://hdl.handle.net/2445/129707 |
| spellingShingle | Insulin controls triacylglycerol synthesis through control of glycerol metabolism and despite increased lipogenesis Ho-Palma, A. C. Glucosa Lípids Insulina Glucose Lipids Insulin |
| status_str | publishedVersion |
| title | Insulin controls triacylglycerol synthesis through control of glycerol metabolism and despite increased lipogenesis |
| title_full | Insulin controls triacylglycerol synthesis through control of glycerol metabolism and despite increased lipogenesis |
| title_fullStr | Insulin controls triacylglycerol synthesis through control of glycerol metabolism and despite increased lipogenesis |
| title_full_unstemmed | Insulin controls triacylglycerol synthesis through control of glycerol metabolism and despite increased lipogenesis |
| title_short | Insulin controls triacylglycerol synthesis through control of glycerol metabolism and despite increased lipogenesis |
| title_sort | Insulin controls triacylglycerol synthesis through control of glycerol metabolism and despite increased lipogenesis |
| topic | Glucosa Lípids Insulina Glucose Lipids Insulin |
| topic_facet | Glucosa Lípids Insulina Glucose Lipids Insulin |
| url | https://hdl.handle.net/2445/129707 |