Occurrence and molecular epidemiology of Giardia duodenalis infection in dog populations in eastern Spain
BACKGROUND: Giardia duodenalis is one of the most common enteric parasites in domestic animals including dogs. Young animals are more prone to the infection, with clinical manifestations ranging from asymptomatic to acute or chronic diarrhoea. Dogs are primarily infected by canine-specific (C-D) ass...
| Autores: | ; ; ; ; ; ; ; |
|---|---|
| Tipo de documento: | Artículo |
| Fecha de publicación: | 2018 |
| País: | España |
| Institución: | Instituto de Salud Carlos III (ISCIII) |
| Repositorio: | Repisalud |
| Lenguaje: | Inglés |
| OAI Identifier: | oai:repisalud.isciii.es:20.500.12105/7055 |
| Acceso en línea: | http://hdl.handle.net/20.500.12105/7055 |
| Nivel de acceso: | Acceso abierto |
| Palabra clave: | Animals Cross-Sectional Studies Dog Diseases Dogs Feces Female Genes, rRNA Giardia lamblia Giardiasis Male Microscopy, Fluorescence Molecular Epidemiology Spain |
| _version_ | 1878735550136975360 |
|---|---|
| author | Adell-Aledón, Manuel |
| author2 | Köster, Pamela Carolina de Lucio, Aida Puente, Paula Hernandez-De-Mingo, Marta Sánchez-Thevenet, Paula Dea-Ayuela, María Auxiliadora Carmena, David |
| author2_role | author author author author author author author |
| author_browse | Adell-Aledón, Manuel Carmena, David Dea-Ayuela, María Auxiliadora Hernandez-De-Mingo, Marta Köster, Pamela Carolina Puente, Paula Sánchez-Thevenet, Paula de Lucio, Aida |
| author_facet | Adell-Aledón, Manuel Köster, Pamela Carolina de Lucio, Aida Puente, Paula Hernandez-De-Mingo, Marta Sánchez-Thevenet, Paula Dea-Ayuela, María Auxiliadora Carmena, David |
| author_role | author |
| collection | Repisalud |
| description | BACKGROUND: Giardia duodenalis is one of the most common enteric parasites in domestic animals including dogs. Young animals are more prone to the infection, with clinical manifestations ranging from asymptomatic to acute or chronic diarrhoea. Dogs are primarily infected by canine-specific (C-D) assemblages of G. duodenalis. However, zoonotic assemblages A and B have been increasingly documented in canine isolates, raising the question of whether and to which extent dogs can act as natural reservoirs of human giardiosis. METHODS: In this cross-sectional epidemiological survey we assessed the molecular diversity of G. duodenalis in dogs in the province of Castellón, Eastern Spain. A total of 348 individual faecal samples from sheltered (n = 218), breeding (n = 24), hunting (n = 68), shepherd (n = 24), and pet (n = 14) dogs were collected between 2014 and 2016. Detection of G. duodenalis cysts in faecal material was carried out by direct fluorescence microscopy as a screening test, whereas a qPCR targeting the small subunit ribosomal RNA gene of the parasite was subsequently used as a confirmatory method. RESULTS: Giardia duodenalis was detected in 36.5% (95% CI: 31.6-41.7%) of dogs. No significant differences in prevalence rates could be demonstrated among dogs according to their sex and geographical origin, but breeding (45.8%; 95% CI: 27.9-64.9%) and sheltered (40.4%; 95% CI: 34.1-47.0%) dogs harboured significantly higher proportions of G. duodenalis. Multi-locus sequence-based genotyping of the glutamate dehydrogenase and β-giardin genes of G. duodenalis allowed the characterization of 35 canine isolates that were unambiguously assigned to assemblages A (14.3%), B (22.9%), C (5.7%), and D (37.1%). A number of inter-assemblage mixed infections including A + B (11.4%), A + D (2.9%), and A + B + D (5.7%) were also identified. CONCLUSIONS: Data presented here are strongly indicative of high infection pressures in kennelled animals. Zoonotic sub-assemblages AII, BIII, and BIV were responsible for a considerable proportion of the G. duodenalis infections detected, but very few of the genotypes identified have been previously documented in Spanish human populations. Although possible, zoonotic transmission between dogs and humans seems an infrequent event in this Spanish region. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_7eed6833dbde2fc12c821423dbc5b5f2 |
| institution | Instituto de Salud Carlos III (ISCIII) |
| instname_str | Instituto de Salud Carlos III (ISCIII) |
| language | eng |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:repisalud.isciii.es:20.500.12105/7055 |
| publishDate | 2018 |
| publishDateSort | 2018 |
| publisher | BioMed Central (BMC) |
| publisherStr | BioMed Central (BMC) |
| reponame_str | Repisalud |
| spelling | Occurrence and molecular epidemiology of Giardia duodenalis infection in dog populations in eastern Spain Adell-Aledón, Manuel Köster, Pamela Carolina de Lucio, Aida Puente, Paula Hernandez-De-Mingo, Marta Sánchez-Thevenet, Paula Dea-Ayuela, María Auxiliadora Carmena, David Animals Cross-Sectional Studies Dog Diseases Dogs Feces Female Genes, rRNA Giardia lamblia Giardiasis Male Microscopy, Fluorescence Molecular Epidemiology Spain BACKGROUND: Giardia duodenalis is one of the most common enteric parasites in domestic animals including dogs. Young animals are more prone to the infection, with clinical manifestations ranging from asymptomatic to acute or chronic diarrhoea. Dogs are primarily infected by canine-specific (C-D) assemblages of G. duodenalis. However, zoonotic assemblages A and B have been increasingly documented in canine isolates, raising the question of whether and to which extent dogs can act as natural reservoirs of human giardiosis. METHODS: In this cross-sectional epidemiological survey we assessed the molecular diversity of G. duodenalis in dogs in the province of Castellón, Eastern Spain. A total of 348 individual faecal samples from sheltered (n = 218), breeding (n = 24), hunting (n = 68), shepherd (n = 24), and pet (n = 14) dogs were collected between 2014 and 2016. Detection of G. duodenalis cysts in faecal material was carried out by direct fluorescence microscopy as a screening test, whereas a qPCR targeting the small subunit ribosomal RNA gene of the parasite was subsequently used as a confirmatory method. RESULTS: Giardia duodenalis was detected in 36.5% (95% CI: 31.6-41.7%) of dogs. No significant differences in prevalence rates could be demonstrated among dogs according to their sex and geographical origin, but breeding (45.8%; 95% CI: 27.9-64.9%) and sheltered (40.4%; 95% CI: 34.1-47.0%) dogs harboured significantly higher proportions of G. duodenalis. Multi-locus sequence-based genotyping of the glutamate dehydrogenase and β-giardin genes of G. duodenalis allowed the characterization of 35 canine isolates that were unambiguously assigned to assemblages A (14.3%), B (22.9%), C (5.7%), and D (37.1%). A number of inter-assemblage mixed infections including A + B (11.4%), A + D (2.9%), and A + B + D (5.7%) were also identified. CONCLUSIONS: Data presented here are strongly indicative of high infection pressures in kennelled animals. Zoonotic sub-assemblages AII, BIII, and BIV were responsible for a considerable proportion of the G. duodenalis infections detected, but very few of the genotypes identified have been previously documented in Spanish human populations. Although possible, zoonotic transmission between dogs and humans seems an infrequent event in this Spanish region. BioMed Central (BMC) http://hdl.handle.net/20.500.12105/7055 |
| spellingShingle | Occurrence and molecular epidemiology of Giardia duodenalis infection in dog populations in eastern Spain Adell-Aledón, Manuel Animals Cross-Sectional Studies Dog Diseases Dogs Feces Female Genes, rRNA Giardia lamblia Giardiasis Male Microscopy, Fluorescence Molecular Epidemiology Spain |
| title | Occurrence and molecular epidemiology of Giardia duodenalis infection in dog populations in eastern Spain |
| title_full | Occurrence and molecular epidemiology of Giardia duodenalis infection in dog populations in eastern Spain |
| title_fullStr | Occurrence and molecular epidemiology of Giardia duodenalis infection in dog populations in eastern Spain |
| title_full_unstemmed | Occurrence and molecular epidemiology of Giardia duodenalis infection in dog populations in eastern Spain |
| title_short | Occurrence and molecular epidemiology of Giardia duodenalis infection in dog populations in eastern Spain |
| title_sort | Occurrence and molecular epidemiology of Giardia duodenalis infection in dog populations in eastern Spain |
| topic | Animals Cross-Sectional Studies Dog Diseases Dogs Feces Female Genes, rRNA Giardia lamblia Giardiasis Male Microscopy, Fluorescence Molecular Epidemiology Spain |
| topic_facet | Animals Cross-Sectional Studies Dog Diseases Dogs Feces Female Genes, rRNA Giardia lamblia Giardiasis Male Microscopy, Fluorescence Molecular Epidemiology Spain |
| url | http://hdl.handle.net/20.500.12105/7055 |