Deletion of the Gene for the Type I Interferon Inhibitor I329L from the Attenuated African Swine Fever Virus OURT88/3 Strain Reduces Protection Induced in Pigs
Live attenuated vaccines are considered to be the fastest route to the development of a safe and efficacious African swine fever (ASF) vaccine. Infection with the naturally attenuated OURT88/3 strain induces protection against challenge with virulent isolates from the same or closely related genotyp...
| Authors: | ; ; ; ; ; |
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| Format: | Article |
| Publication date: | 2020 |
| Country: | España |
| Institution: | Universitat Autònoma de Barcelona |
| Repository: | Dipòsit Digital de Documents de la UAB |
| Language: | English |
| OAI Identifier: | oai:ddd.uab.cat:252639 |
| Online Access: | https://ddd.uab.cat/record/252639 https://dx.doi.org/urn:doi:10.3390/vaccines8020262 |
| Access Level: | Open access |
| Keyword: | African swine fever virus Interferon I329L Vaccine |
| _version_ | 1878735501801816064 |
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| author | Reis, Ana Luisa |
| author2 | Goatley, Lynnette C. Jabbar, Tamara López Fernandez, Elisabet|||0000-0003-4253-330X Rathakrishnan, Anusyah Dixon, Linda|||0000-0003-3845-3016 |
| author2_role | author author author author author |
| author_browse | Dixon, Linda|||0000-0003-3845-3016 Goatley, Lynnette C. Jabbar, Tamara López Fernandez, Elisabet|||0000-0003-4253-330X Rathakrishnan, Anusyah Reis, Ana Luisa |
| author_facet | Reis, Ana Luisa Goatley, Lynnette C. Jabbar, Tamara López Fernandez, Elisabet|||0000-0003-4253-330X Rathakrishnan, Anusyah Dixon, Linda|||0000-0003-3845-3016 |
| author_role | author |
| collection | Dipòsit Digital de Documents de la UAB |
| description | Live attenuated vaccines are considered to be the fastest route to the development of a safe and efficacious African swine fever (ASF) vaccine. Infection with the naturally attenuated OURT88/3 strain induces protection against challenge with virulent isolates from the same or closely related genotypes. However, adverse clinical signs following immunisation have been observed. Here, we attempted to increase the OURT88/3 safety profile by deleting I329L, a gene previously shown to inhibit the host innate immune response. The resulting virus, OURT88/3ΔI329L, was tested in vitro to evaluate the replication and expression of type I interferon (IFN) and in vivo by immunisation and lethal challenge experiments in pigs. No differences were observed regarding replication; however, increased amounts of both IFN-β and IFN- α were observed in macrophages infected with the deletion mutant virus. Unexpectedly, the deletion of I329L markedly reduced protection against challenge with the virulent OURT88/1 isolate. This was associated with a decrease in both antibody levels against VP72 and the number of IFN-γ-producing cells in the blood of non-protected animals. Furthermore, a significant increase in IL-10 levels in serum was observed in pigs immunised with OURT88/3ΔI329L following challenge. Interestingly, the deletion of the I329L gene failed to attenuate the virulent Georgia/2007 isolate. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_7df006cd6fe4e8b0fb98415ef5f3d07f |
| institution | Universitat Autònoma de Barcelona |
| instname_str | Universitat Autònoma de Barcelona |
| language | eng |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:ddd.uab.cat:252639 |
| publishDate | 2020 |
| publishDateSort | 2020 |
| reponame_str | Dipòsit Digital de Documents de la UAB |
| spelling | Deletion of the Gene for the Type I Interferon Inhibitor I329L from the Attenuated African Swine Fever Virus OURT88/3 Strain Reduces Protection Induced in Pigs Reis, Ana Luisa Goatley, Lynnette C. Jabbar, Tamara López Fernandez, Elisabet|||0000-0003-4253-330X Rathakrishnan, Anusyah Dixon, Linda|||0000-0003-3845-3016 African swine fever virus Interferon I329L Vaccine Live attenuated vaccines are considered to be the fastest route to the development of a safe and efficacious African swine fever (ASF) vaccine. Infection with the naturally attenuated OURT88/3 strain induces protection against challenge with virulent isolates from the same or closely related genotypes. However, adverse clinical signs following immunisation have been observed. Here, we attempted to increase the OURT88/3 safety profile by deleting I329L, a gene previously shown to inhibit the host innate immune response. The resulting virus, OURT88/3ΔI329L, was tested in vitro to evaluate the replication and expression of type I interferon (IFN) and in vivo by immunisation and lethal challenge experiments in pigs. No differences were observed regarding replication; however, increased amounts of both IFN-β and IFN- α were observed in macrophages infected with the deletion mutant virus. Unexpectedly, the deletion of I329L markedly reduced protection against challenge with the virulent OURT88/1 isolate. This was associated with a decrease in both antibody levels against VP72 and the number of IFN-γ-producing cells in the blood of non-protected animals. Furthermore, a significant increase in IL-10 levels in serum was observed in pigs immunised with OURT88/3ΔI329L following challenge. Interestingly, the deletion of the I329L gene failed to attenuate the virulent Georgia/2007 isolate. https://ddd.uab.cat/record/252639 https://dx.doi.org/urn:doi:10.3390/vaccines8020262 |
| spellingShingle | Deletion of the Gene for the Type I Interferon Inhibitor I329L from the Attenuated African Swine Fever Virus OURT88/3 Strain Reduces Protection Induced in Pigs Reis, Ana Luisa African swine fever virus Interferon I329L Vaccine |
| title | Deletion of the Gene for the Type I Interferon Inhibitor I329L from the Attenuated African Swine Fever Virus OURT88/3 Strain Reduces Protection Induced in Pigs |
| title_full | Deletion of the Gene for the Type I Interferon Inhibitor I329L from the Attenuated African Swine Fever Virus OURT88/3 Strain Reduces Protection Induced in Pigs |
| title_fullStr | Deletion of the Gene for the Type I Interferon Inhibitor I329L from the Attenuated African Swine Fever Virus OURT88/3 Strain Reduces Protection Induced in Pigs |
| title_full_unstemmed | Deletion of the Gene for the Type I Interferon Inhibitor I329L from the Attenuated African Swine Fever Virus OURT88/3 Strain Reduces Protection Induced in Pigs |
| title_short | Deletion of the Gene for the Type I Interferon Inhibitor I329L from the Attenuated African Swine Fever Virus OURT88/3 Strain Reduces Protection Induced in Pigs |
| title_sort | Deletion of the Gene for the Type I Interferon Inhibitor I329L from the Attenuated African Swine Fever Virus OURT88/3 Strain Reduces Protection Induced in Pigs |
| topic | African swine fever virus Interferon I329L Vaccine |
| topic_facet | African swine fever virus Interferon I329L Vaccine |
| url | https://ddd.uab.cat/record/252639 https://dx.doi.org/urn:doi:10.3390/vaccines8020262 |