Antibiotic resistance genes in phage particles isolated from human faeces and induced from clinical bacterial isolates
Phage particles have emerged as elements with the potential to mobilise antibiotic resistance genes (ARGs) in different environments, including the intestinal habitat. This study aimed to determine the occurrence of ARGs in phage particles present in faecal matter and induced from strains isolated f...
| Authors: | ; ; ; ; ; ; ; ; ; |
|---|---|
| Format: | Article |
| Publication date: | 2018 |
| Country: | España |
| Institution: | Universitat Autònoma de Barcelona |
| Repository: | Dipòsit Digital de Documents de la UAB |
| Language: | English |
| OAI Identifier: | oai:ddd.uab.cat:285188 |
| Online Access: | https://ddd.uab.cat/record/285188 https://dx.doi.org/urn:doi:10.1016/j.ijantimicag.2017.11.014 |
| Access Level: | Open access |
| Keyword: | Antibiotic resistance Bacteriophage Faeces Horizontal gene transfer Transduction SDG 3 - Good Health and Well-being |
| _version_ | 1878735362826698752 |
|---|---|
| author | Brown-Jaque, Maryury |
| author2 | Calero-Cáceres, William Espinal, Paula|||0000-0002-1014-8936 Rodríguez-Navarro, Judith|||0000-0002-2855-254X Miró, Elisenda|||0000-0003-2781-6926 González-López, Juanjo|||0000-0003-2419-5909 Cornejo-Sánchez, Thais|||0000-0002-1350-0413 Hurtado, Juan Carlos Navarro Risueño, Ferran|||0000-0002-4302-2838 Muniesa, Maite|||0000-0001-5549-1394 |
| author2_role | author author author author author author author author author |
| author_browse | Brown-Jaque, Maryury Calero-Cáceres, William Cornejo-Sánchez, Thais|||0000-0002-1350-0413 Espinal, Paula|||0000-0002-1014-8936 González-López, Juanjo|||0000-0003-2419-5909 Hurtado, Juan Carlos Miró, Elisenda|||0000-0003-2781-6926 Muniesa, Maite|||0000-0001-5549-1394 Navarro Risueño, Ferran|||0000-0002-4302-2838 Rodríguez-Navarro, Judith|||0000-0002-2855-254X |
| author_facet | Brown-Jaque, Maryury Calero-Cáceres, William Espinal, Paula|||0000-0002-1014-8936 Rodríguez-Navarro, Judith|||0000-0002-2855-254X Miró, Elisenda|||0000-0003-2781-6926 González-López, Juanjo|||0000-0003-2419-5909 Cornejo-Sánchez, Thais|||0000-0002-1350-0413 Hurtado, Juan Carlos Navarro Risueño, Ferran|||0000-0002-4302-2838 Muniesa, Maite|||0000-0001-5549-1394 |
| author_role | author |
| collection | Dipòsit Digital de Documents de la UAB |
| description | Phage particles have emerged as elements with the potential to mobilise antibiotic resistance genes (ARGs) in different environments, including the intestinal habitat. This study aimed to determine the occurrence of ARGs in phage particles present in faecal matter and induced from strains isolated from faeces. Nine ARGs (blaTEM, blaCTX-M-1 group, blaCTX-M-9 group, blaOXA-48, qnrA, qnrS, mecA, sul1 and armA) were quantified by qPCR in the phage DNA fractions of 150 faecal samples obtained from healthy individuals who had not received antibiotic treatment or travelled abroad in the 3 months prior to sample collection. On the suspicion that the detected particles originated from bacterial flora, 82 Escherichia coli and Klebsiella pneumoniae isolates possessing at least one identified ARG (blaTEM, blaCTX-M-1 group, blaCTX-M-9 group, armA, qnrA, qnrS and sul1) were isolated and their capacity to produce phage particles carrying these ARGs following induction was evaluated. Of 150 samples, 72.7% were positive for at least one ARG, with blaTEM and blaCTX-M-9 group being the most prevalent and abundant. Of the 82 isolates, 51 (62%) showed an increase in the number of copies of the respective ARG in the phage fraction following induction, with blaTEM, blaCTX-M-1 group, blaCTX-M-9 group and sul1 being the most abundant. Phages induced from the isolates were further purified and visualised using microscopy and their DNA showed ARG levels of up to 1010 gene copies/mL. This study highlights the abundance of phage particles harbouring ARGs and indicates that bacterial strains in the intestinal habitat could be source of these particles. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_7b3f64fa2ef7222bfa41c56b2f71c2ee |
| institution | Universitat Autònoma de Barcelona |
| instname_str | Universitat Autònoma de Barcelona |
| language | eng |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:ddd.uab.cat:285188 |
| publishDate | 2018 |
| publishDateSort | 2018 |
| reponame_str | Dipòsit Digital de Documents de la UAB |
| spelling | Antibiotic resistance genes in phage particles isolated from human faeces and induced from clinical bacterial isolates Brown-Jaque, Maryury Calero-Cáceres, William Espinal, Paula|||0000-0002-1014-8936 Rodríguez-Navarro, Judith|||0000-0002-2855-254X Miró, Elisenda|||0000-0003-2781-6926 González-López, Juanjo|||0000-0003-2419-5909 Cornejo-Sánchez, Thais|||0000-0002-1350-0413 Hurtado, Juan Carlos Navarro Risueño, Ferran|||0000-0002-4302-2838 Muniesa, Maite|||0000-0001-5549-1394 Antibiotic resistance Bacteriophage Faeces Horizontal gene transfer Transduction SDG 3 - Good Health and Well-being Phage particles have emerged as elements with the potential to mobilise antibiotic resistance genes (ARGs) in different environments, including the intestinal habitat. This study aimed to determine the occurrence of ARGs in phage particles present in faecal matter and induced from strains isolated from faeces. Nine ARGs (blaTEM, blaCTX-M-1 group, blaCTX-M-9 group, blaOXA-48, qnrA, qnrS, mecA, sul1 and armA) were quantified by qPCR in the phage DNA fractions of 150 faecal samples obtained from healthy individuals who had not received antibiotic treatment or travelled abroad in the 3 months prior to sample collection. On the suspicion that the detected particles originated from bacterial flora, 82 Escherichia coli and Klebsiella pneumoniae isolates possessing at least one identified ARG (blaTEM, blaCTX-M-1 group, blaCTX-M-9 group, armA, qnrA, qnrS and sul1) were isolated and their capacity to produce phage particles carrying these ARGs following induction was evaluated. Of 150 samples, 72.7% were positive for at least one ARG, with blaTEM and blaCTX-M-9 group being the most prevalent and abundant. Of the 82 isolates, 51 (62%) showed an increase in the number of copies of the respective ARG in the phage fraction following induction, with blaTEM, blaCTX-M-1 group, blaCTX-M-9 group and sul1 being the most abundant. Phages induced from the isolates were further purified and visualised using microscopy and their DNA showed ARG levels of up to 1010 gene copies/mL. This study highlights the abundance of phage particles harbouring ARGs and indicates that bacterial strains in the intestinal habitat could be source of these particles. https://ddd.uab.cat/record/285188 https://dx.doi.org/urn:doi:10.1016/j.ijantimicag.2017.11.014 |
| spellingShingle | Antibiotic resistance genes in phage particles isolated from human faeces and induced from clinical bacterial isolates Brown-Jaque, Maryury Antibiotic resistance Bacteriophage Faeces Horizontal gene transfer Transduction SDG 3 - Good Health and Well-being |
| title | Antibiotic resistance genes in phage particles isolated from human faeces and induced from clinical bacterial isolates |
| title_full | Antibiotic resistance genes in phage particles isolated from human faeces and induced from clinical bacterial isolates |
| title_fullStr | Antibiotic resistance genes in phage particles isolated from human faeces and induced from clinical bacterial isolates |
| title_full_unstemmed | Antibiotic resistance genes in phage particles isolated from human faeces and induced from clinical bacterial isolates |
| title_short | Antibiotic resistance genes in phage particles isolated from human faeces and induced from clinical bacterial isolates |
| title_sort | Antibiotic resistance genes in phage particles isolated from human faeces and induced from clinical bacterial isolates |
| topic | Antibiotic resistance Bacteriophage Faeces Horizontal gene transfer Transduction SDG 3 - Good Health and Well-being |
| topic_facet | Antibiotic resistance Bacteriophage Faeces Horizontal gene transfer Transduction SDG 3 - Good Health and Well-being |
| url | https://ddd.uab.cat/record/285188 https://dx.doi.org/urn:doi:10.1016/j.ijantimicag.2017.11.014 |