Possible role of highly activated mucosal NK cells against viral respiratory infections in children undergoing haematopoietic stem cell transplantation
Infection is the leading cause of non-relapse-related mortality after allogeneic haematopoietic stem cell transplantation (HSCT). Altered functions of immune cells in nasal secretions may influence post HSCT susceptibility to viral respiratory infections. In this prospective study, we determined T a...
| Authors: | ; ; ; ; ; ; ; ; ; ; ; |
|---|---|
| Format: | Article |
| Publication date: | 2019 |
| Country: | España |
| Institution: | Universidad Autónoma de Madrid |
| Repository: | Biblos-e Archivo. Repositorio Institucional de la UAM |
| Language: | English |
| OAI Identifier: | oai:repositorio.uam.es:10486/691520 |
| Online Access: | http://hdl.handle.net/10486/691520 https://dx.doi.org/10.1038/s41598-019-55398-y |
| Access Level: | Open access |
| Keyword: | Mucosal NK cells Infection Cell transplantation (HSCT) Children Haematopoietic stem Medicina |
| _version_ | 1878735349094547456 |
|---|---|
| author | Vela, María |
| author2 | Rosal, Teresa del Pérez Martínez, Antonio Valentín, Jaime Casas, Inmaculada Pozo, Francisco Reinoso Barbero, Francisco Bueno, David Corral, Dolores Méndez Echevarría, Ana María Mozo, Yasmina Calvo Rey, Cristina |
| author2_role | author author author author author author author author author author author |
| author_browse | Bueno, David Calvo Rey, Cristina Casas, Inmaculada Corral, Dolores Mozo, Yasmina Méndez Echevarría, Ana María Pozo, Francisco Pérez Martínez, Antonio Reinoso Barbero, Francisco Rosal, Teresa del Valentín, Jaime Vela, María |
| author_facet | Vela, María Rosal, Teresa del Pérez Martínez, Antonio Valentín, Jaime Casas, Inmaculada Pozo, Francisco Reinoso Barbero, Francisco Bueno, David Corral, Dolores Méndez Echevarría, Ana María Mozo, Yasmina Calvo Rey, Cristina |
| author_role | author |
| collection | Biblos-e Archivo. Repositorio Institucional de la UAM |
| description | Infection is the leading cause of non-relapse-related mortality after allogeneic haematopoietic stem cell transplantation (HSCT). Altered functions of immune cells in nasal secretions may influence post HSCT susceptibility to viral respiratory infections. In this prospective study, we determined T and NK cell numbers together with NK activation status in nasopharyngeal aspirates (NPA) in HSCT recipients and healthy controls using multiparametric flow cytometry. We also determined by polymerase chain reaction (PCR) the presence of 16 respiratory viruses. Samples were collected pre-HSCT, at day 0, +10, +20 and +30 after HSCT. Peripheral blood (PB) was also analyzed to determine T and NK cell numbers. A total of 27 pediatric HSCT recipients were enrolled and 16 of them had at least one viral detection (60%). Rhinovirus was the most frequent pathogen (84% of positive NPAs). NPAs of patients contained fewer T and NK cells compared to healthy controls (p = 0.0132 and p = 0.120, respectively). Viral PCR + patients showed higher NK cell number in their NPAs. The activating receptors repertoire expressed by NK cells was also higher in NPA samples, especially NKp44 and NKp46. Our study supports NK cells relevance for the immune defense against respiratory viruses in HSCT recipients |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_7af08226e1bedfb75c19c808f2efaed0 |
| institution | Universidad Autónoma de Madrid |
| instname_str | Universidad Autónoma de Madrid |
| language | eng |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:repositorio.uam.es:10486/691520 |
| publishDate | 2019 |
| publishDateSort | 2019 |
| publisher | Nature Research (part of Springer Nature) |
| publisherStr | Nature Research (part of Springer Nature) |
| reponame_str | Biblos-e Archivo. Repositorio Institucional de la UAM |
| spelling | Possible role of highly activated mucosal NK cells against viral respiratory infections in children undergoing haematopoietic stem cell transplantation Vela, María Rosal, Teresa del Pérez Martínez, Antonio Valentín, Jaime Casas, Inmaculada Pozo, Francisco Reinoso Barbero, Francisco Bueno, David Corral, Dolores Méndez Echevarría, Ana María Mozo, Yasmina Calvo Rey, Cristina Mucosal NK cells Infection Cell transplantation (HSCT) Children Haematopoietic stem Medicina Infection is the leading cause of non-relapse-related mortality after allogeneic haematopoietic stem cell transplantation (HSCT). Altered functions of immune cells in nasal secretions may influence post HSCT susceptibility to viral respiratory infections. In this prospective study, we determined T and NK cell numbers together with NK activation status in nasopharyngeal aspirates (NPA) in HSCT recipients and healthy controls using multiparametric flow cytometry. We also determined by polymerase chain reaction (PCR) the presence of 16 respiratory viruses. Samples were collected pre-HSCT, at day 0, +10, +20 and +30 after HSCT. Peripheral blood (PB) was also analyzed to determine T and NK cell numbers. A total of 27 pediatric HSCT recipients were enrolled and 16 of them had at least one viral detection (60%). Rhinovirus was the most frequent pathogen (84% of positive NPAs). NPAs of patients contained fewer T and NK cells compared to healthy controls (p = 0.0132 and p = 0.120, respectively). Viral PCR + patients showed higher NK cell number in their NPAs. The activating receptors repertoire expressed by NK cells was also higher in NPA samples, especially NKp44 and NKp46. Our study supports NK cells relevance for the immune defense against respiratory viruses in HSCT recipients This work was supported in part by the National Health Service of Spain, Instituto de Salud Carlos III (ISCIII), FONDOS FEDER grant (FIS) PI18/01301, CRIS Foundation to Beat Cancer, Patients’ Support Associations Fundación Mari Paz Jiménez Casado and La Sonrisa de Álex and a Small Grant Award from the European Society for Paediatric Infectious Diseases Nature Research (part of Springer Nature) http://hdl.handle.net/10486/691520 https://dx.doi.org/10.1038/s41598-019-55398-y |
| spellingShingle | Possible role of highly activated mucosal NK cells against viral respiratory infections in children undergoing haematopoietic stem cell transplantation Vela, María Mucosal NK cells Infection Cell transplantation (HSCT) Children Haematopoietic stem Medicina |
| title | Possible role of highly activated mucosal NK cells against viral respiratory infections in children undergoing haematopoietic stem cell transplantation |
| title_full | Possible role of highly activated mucosal NK cells against viral respiratory infections in children undergoing haematopoietic stem cell transplantation |
| title_fullStr | Possible role of highly activated mucosal NK cells against viral respiratory infections in children undergoing haematopoietic stem cell transplantation |
| title_full_unstemmed | Possible role of highly activated mucosal NK cells against viral respiratory infections in children undergoing haematopoietic stem cell transplantation |
| title_short | Possible role of highly activated mucosal NK cells against viral respiratory infections in children undergoing haematopoietic stem cell transplantation |
| title_sort | Possible role of highly activated mucosal NK cells against viral respiratory infections in children undergoing haematopoietic stem cell transplantation |
| topic | Mucosal NK cells Infection Cell transplantation (HSCT) Children Haematopoietic stem Medicina |
| topic_facet | Mucosal NK cells Infection Cell transplantation (HSCT) Children Haematopoietic stem Medicina |
| url | http://hdl.handle.net/10486/691520 https://dx.doi.org/10.1038/s41598-019-55398-y |