Effect of Operating Variables and Kinetics of the Lipase Catalyzed Transesterification of Ethylene Carbonate and Glycerol
Glycerol carbonate (GC) is a value-added product originating from the valorization of widely available glycerol (Gly), a side stream from the production of biodiesel. Here we approach the production of this chemical comparing two reactions based on the transesterification of Gly with dimethyl carbon...
| Authors: | ; ; ; ; ; |
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| Format: | Article |
| Publication date: | 2018 |
| Country: | España |
| Institution: | Universidad Complutense de Madrid (UCM) |
| Repository: | Docta Complutense |
| Language: | English |
| OAI Identifier: | oai:docta.ucm.es:20.500.14352/12602 |
| Online Access: | https://hdl.handle.net/20.500.14352/12602 |
| Access Level: | Open access |
| Keyword: | glycerol glycerol carbonate Novozym 435 Lipozyme TL 100 L Eversa Transform 2.0 kinetic model Ingeniería química Materiales 3303 Ingeniería y Tecnología Químicas 3312 Tecnología de Materiales |
| _version_ | 1878734951535345664 |
|---|---|
| author | Gutiérrez Lázaro, Ana |
| author2 | Velasco Conde, Daniel Boldrini, Diego Yustos Cuesta, Pedro Esteban, Jesus Ladero Galán, Miguel |
| author2_role | author author author author author |
| author_browse | Boldrini, Diego Esteban, Jesus Gutiérrez Lázaro, Ana Ladero Galán, Miguel Velasco Conde, Daniel Yustos Cuesta, Pedro |
| author_facet | Gutiérrez Lázaro, Ana Velasco Conde, Daniel Boldrini, Diego Yustos Cuesta, Pedro Esteban, Jesus Ladero Galán, Miguel |
| author_role | author |
| collection | Docta Complutense |
| description | Glycerol carbonate (GC) is a value-added product originating from the valorization of widely available glycerol (Gly), a side stream from the production of biodiesel. Here we approach the production of this chemical comparing two reactions based on the transesterification of Gly with dimethyl carbonate (DMC) and ethylene carbonate (EC). When using DMC, it was observed that the free enzyme CALB (lipase B from Candida antarctica) gave the best results, whereas Eversa Transform (a genetic modification of Thermomyces lanuginosus lipase) performed better than the rest if EC was the reagent. With the selected catalysts, their immobilized analogous enzymes Novozym 435 and Lypozyme TL IM, respectively, were also tested. Observing that the yields for the reaction with EC were significantly faster, other operating variables were evaluated, resulting the best performance using a closed system, tert-butanol as solvent, a concentration of enzyme Eversa Transform of 3% w/w, a molar excess of EC:Gly of 9:1 and a temperature of 60 °C. Finally, several runs were conducted at different temperatures and molar ratios of EC:Gly, fitting a kinetic model to all experimental data for the reaction catalyzed with Eversa Transform. This model included the bimolecular transesterification reaction of Gly and EC catalyzed by the lipase and a reversible ring-opening polymerization of EC. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_730d184776eae4e59fc94f85a46c6eeb |
| institution | Universidad Complutense de Madrid (UCM) |
| instname_str | Universidad Complutense de Madrid (UCM) |
| language | eng |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:docta.ucm.es:20.500.14352/12602 |
| publishDate | 2018 |
| publishDateSort | 2018 |
| publisher | MDPI |
| publisherStr | MDPI |
| reponame_str | Docta Complutense |
| spelling | Effect of Operating Variables and Kinetics of the Lipase Catalyzed Transesterification of Ethylene Carbonate and Glycerol Gutiérrez Lázaro, Ana Velasco Conde, Daniel Boldrini, Diego Yustos Cuesta, Pedro Esteban, Jesus Ladero Galán, Miguel glycerol glycerol carbonate Novozym 435 Lipozyme TL 100 L Eversa Transform 2.0 kinetic model Ingeniería química Materiales 3303 Ingeniería y Tecnología Químicas 3312 Tecnología de Materiales Glycerol carbonate (GC) is a value-added product originating from the valorization of widely available glycerol (Gly), a side stream from the production of biodiesel. Here we approach the production of this chemical comparing two reactions based on the transesterification of Gly with dimethyl carbonate (DMC) and ethylene carbonate (EC). When using DMC, it was observed that the free enzyme CALB (lipase B from Candida antarctica) gave the best results, whereas Eversa Transform (a genetic modification of Thermomyces lanuginosus lipase) performed better than the rest if EC was the reagent. With the selected catalysts, their immobilized analogous enzymes Novozym 435 and Lypozyme TL IM, respectively, were also tested. Observing that the yields for the reaction with EC were significantly faster, other operating variables were evaluated, resulting the best performance using a closed system, tert-butanol as solvent, a concentration of enzyme Eversa Transform of 3% w/w, a molar excess of EC:Gly of 9:1 and a temperature of 60 °C. Finally, several runs were conducted at different temperatures and molar ratios of EC:Gly, fitting a kinetic model to all experimental data for the reaction catalyzed with Eversa Transform. This model included the bimolecular transesterification reaction of Gly and EC catalyzed by the lipase and a reversible ring-opening polymerization of EC. MDPI https://hdl.handle.net/20.500.14352/12602 |
| spellingShingle | Effect of Operating Variables and Kinetics of the Lipase Catalyzed Transesterification of Ethylene Carbonate and Glycerol Gutiérrez Lázaro, Ana glycerol glycerol carbonate Novozym 435 Lipozyme TL 100 L Eversa Transform 2.0 kinetic model Ingeniería química Materiales 3303 Ingeniería y Tecnología Químicas 3312 Tecnología de Materiales |
| title | Effect of Operating Variables and Kinetics of the Lipase Catalyzed Transesterification of Ethylene Carbonate and Glycerol |
| title_full | Effect of Operating Variables and Kinetics of the Lipase Catalyzed Transesterification of Ethylene Carbonate and Glycerol |
| title_fullStr | Effect of Operating Variables and Kinetics of the Lipase Catalyzed Transesterification of Ethylene Carbonate and Glycerol |
| title_full_unstemmed | Effect of Operating Variables and Kinetics of the Lipase Catalyzed Transesterification of Ethylene Carbonate and Glycerol |
| title_short | Effect of Operating Variables and Kinetics of the Lipase Catalyzed Transesterification of Ethylene Carbonate and Glycerol |
| title_sort | Effect of Operating Variables and Kinetics of the Lipase Catalyzed Transesterification of Ethylene Carbonate and Glycerol |
| topic | glycerol glycerol carbonate Novozym 435 Lipozyme TL 100 L Eversa Transform 2.0 kinetic model Ingeniería química Materiales 3303 Ingeniería y Tecnología Químicas 3312 Tecnología de Materiales |
| topic_facet | glycerol glycerol carbonate Novozym 435 Lipozyme TL 100 L Eversa Transform 2.0 kinetic model Ingeniería química Materiales 3303 Ingeniería y Tecnología Químicas 3312 Tecnología de Materiales |
| url | https://hdl.handle.net/20.500.14352/12602 |