The dynamics of mitochondrial Ca2+ fluxes
Producción Científica
| Authors: | ; ; ; ; ; ; |
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| Format: | Article |
| Status: | Published version |
| Publication date: | 2014 |
| Country: | España |
| Institution: | Universidad de Valladolid |
| Repository: | UVaDOC. Repositorio Documental de la Universidad de Valladolid |
| OAI Identifier: | oai:uvadoc.uva.es:10324/67171 |
| Online Access: | https://doi.org/10.1016/j.bbabio.2010.06.008 https://uvadoc.uva.es/handle/10324/67171 |
| Access Level: | Open access |
| Keyword: | Ca2+ fluxes Ca2+ dynamics Ca2+ buffering Mitochondria Aequorin |
| _version_ | 1878734536633745408 |
|---|---|
| author | Fuente Pérez, Sergio De La |
| author2 | Montenegro, Pablo Fonteriz García, Rosalba Inés Moreno Díaz-Calderón, Alfredo Domínguez Lobatón, María Carmen Montero Zoccola, María Teresa Álvarez Martín, Javier |
| author2_role | author author author author author author |
| author_browse | Domínguez Lobatón, María Carmen Fonteriz García, Rosalba Inés Fuente Pérez, Sergio De La Montenegro, Pablo Montero Zoccola, María Teresa Moreno Díaz-Calderón, Alfredo Álvarez Martín, Javier |
| author_facet | Fuente Pérez, Sergio De La Montenegro, Pablo Fonteriz García, Rosalba Inés Moreno Díaz-Calderón, Alfredo Domínguez Lobatón, María Carmen Montero Zoccola, María Teresa Álvarez Martín, Javier |
| author_role | author |
| collection | UVaDOC. Repositorio Documental de la Universidad de Valladolid |
| description | Producción Científica |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_6baa8aac6a2a1ac07cf83c19f7cc0d2b |
| institution | Universidad de Valladolid |
| instname_str | Universidad de Valladolid |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:uvadoc.uva.es:10324/67171 |
| publishDate | 2014 |
| publishDateSort | 2014 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| reponame_str | UVaDOC. Repositorio Documental de la Universidad de Valladolid |
| spelling | The dynamics of mitochondrial Ca2+ fluxes Fuente Pérez, Sergio De La Montenegro, Pablo Fonteriz García, Rosalba Inés Moreno Díaz-Calderón, Alfredo Domínguez Lobatón, María Carmen Montero Zoccola, María Teresa Álvarez Martín, Javier Ca2+ fluxes Ca2+ dynamics Ca2+ buffering Mitochondria Aequorin Producción Científica MICU1 (Ca2+ uptake protein 1, mitochondrial) is an important regulator of the MCU (Ca2+ uniporter protein, mitochondrial) that has been shown recently to act as a gatekeeper of the MCU at low [Ca2+]c (cytosolic [Ca2+]). In the present study we have investigated in detail the dynamics of MCU activity after shRNA-knockdown of MICU1 and we have found several new interesting properties. In MICU1-knockdown cells, the rate of mitochondrial Ca2+ uptake was largely increased at a low [Ca2+]c (<2 μM), but it was decreased at a high [Ca2+]c (>4 μM). In the 2-4 μM range a mixed behaviour was observed, where mitochondrial Ca2+ uptake started earlier in the MICU1-silenced cells, but at a lower rate than in the controls. The sensitivity of Ca2+ uptake to Ruthenium Red and Ru360 was similar at both high and low [Ca2+]c, indicating that the same Ca2+ pathway was operating in both cases. The increased Ca2+-uptake rate observed at a [Ca2+]c below 2 μM was transient and became inhibited during Ca2+ entry. Development of this inhibition was slow, requiring 5 min for completion, and was hardly reversible. Therefore MICU1 acts both as a MCU gatekeeper at low [Ca2+]c and as a cofactor necessary to reach the maximum Ca2+-uptake rate at high [Ca2+]c. Moreover, in the absence of MICU1, the MCU becomes sensitive to a slow-developing inhibition that requires prolonged increases in [Ca2+]c in the low micromolar range. Elsevier https://doi.org/10.1016/j.bbabio.2010.06.008 https://uvadoc.uva.es/handle/10324/67171 |
| spellingShingle | The dynamics of mitochondrial Ca2+ fluxes Fuente Pérez, Sergio De La Ca2+ fluxes Ca2+ dynamics Ca2+ buffering Mitochondria Aequorin |
| status_str | publishedVersion |
| title | The dynamics of mitochondrial Ca2+ fluxes |
| title_full | The dynamics of mitochondrial Ca2+ fluxes |
| title_fullStr | The dynamics of mitochondrial Ca2+ fluxes |
| title_full_unstemmed | The dynamics of mitochondrial Ca2+ fluxes |
| title_short | The dynamics of mitochondrial Ca2+ fluxes |
| title_sort | The dynamics of mitochondrial Ca2+ fluxes |
| topic | Ca2+ fluxes Ca2+ dynamics Ca2+ buffering Mitochondria Aequorin |
| topic_facet | Ca2+ fluxes Ca2+ dynamics Ca2+ buffering Mitochondria Aequorin |
| url | https://doi.org/10.1016/j.bbabio.2010.06.008 https://uvadoc.uva.es/handle/10324/67171 |