Micro RNA profiles in colostrum exosomes obtained from primiparous or multiparous dairy cows
Colostrum is rich in membranous vesicles of endocytic origin named exosomes, with proteins, lipids, RNA, and/or DNA cargos which can play different roles in physiological processes. Like other colostrum bioactive compounds, exosomes could be also influenced by individual characteristics. The objecti...
| Authors: | ; ; ; ; |
|---|---|
| Format: | Article |
| Publication date: | 2024 |
| Country: | España |
| Institution: | Institut de Recerca i Tecnologia Agroalimentàries (IRTA) |
| Repository: | IRTA Pubpro. Open Digital Archive |
| OAI Identifier: | oai:repositori.irta.cat:20.500.12327/3416 |
| Online Access: | http://hdl.handle.net/20.500.12327/3416 https://doi.org/10.3389/fvets.2024.1463342 |
| Access Level: | Open access |
| Keyword: | 636 |
| _version_ | 1878734388463665152 |
|---|---|
| author | Terre, Marta |
| author2 | Alomar, Oscar Garcia-Fruitos, Elena Fàbregas, Francesc Bach, Alex |
| author2_role | author author author author |
| author_browse | Alomar, Oscar Bach, Alex Fàbregas, Francesc Garcia-Fruitos, Elena Terre, Marta |
| author_facet | Terre, Marta Alomar, Oscar Garcia-Fruitos, Elena Fàbregas, Francesc Bach, Alex |
| author_role | author |
| collection | IRTA Pubpro. Open Digital Archive |
| description | Colostrum is rich in membranous vesicles of endocytic origin named exosomes, with proteins, lipids, RNA, and/or DNA cargos which can play different roles in physiological processes. Like other colostrum bioactive compounds, exosomes could be also influenced by individual characteristics. The objective of the study was to characterize miRNA cargo of colostrum exosomes from primiparous and multiparous cows in different farms. Twenty-seven colostrum samples of clinically healthy Holstein cows (11 primiparous and 16 multiparous) from 3 different farms were obtained and frozen. After thawing, exosomes were isolated following an ultracentrifugation protocol, and characterized morphologically. Particle size distribution and western immunoblotting were also analyzMaed. After RNA extraction, miRNAs were sequenced and analyzed to assess potential differences in profiles between primiparous and multiparous cows from different farms. Fourteen miRNA were upregulated and 11 miRNAs downregulated in primiparous compared with multiparous cows. Most of the miRNA differences between primiparous and multiparous cows regulate the gene expression of factors involved in mammary gland development and differentiation, and lipogenesis. In addition, miRNAs from one of the farms showed 8 miRNAs downregulated and 12 upregulated compared with the other 2 farms, independently of parity. Differences in miRNA between farms were mainly associated with immune and inflammatory-related genes. In conclusion, miRNA cargos of bovine colostrum exosomes differ in primiparous and multiparous cows, and some on-farm practices might also determine the content and activity of miRNA in colostrum exosomes. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_6906fa7c2d271e84d3da5b7b8d2fae87 |
| institution | Institut de Recerca i Tecnologia Agroalimentàries (IRTA) |
| instname_str | Institut de Recerca i Tecnologia Agroalimentàries (IRTA) |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:repositori.irta.cat:20.500.12327/3416 |
| publishDate | 2024 |
| publishDateSort | 2024 |
| publisher | Frontiers Media |
| publisherStr | Frontiers Media |
| reponame_str | IRTA Pubpro. Open Digital Archive |
| spelling | Micro RNA profiles in colostrum exosomes obtained from primiparous or multiparous dairy cows Terre, Marta Alomar, Oscar Garcia-Fruitos, Elena Fàbregas, Francesc Bach, Alex 636 Colostrum is rich in membranous vesicles of endocytic origin named exosomes, with proteins, lipids, RNA, and/or DNA cargos which can play different roles in physiological processes. Like other colostrum bioactive compounds, exosomes could be also influenced by individual characteristics. The objective of the study was to characterize miRNA cargo of colostrum exosomes from primiparous and multiparous cows in different farms. Twenty-seven colostrum samples of clinically healthy Holstein cows (11 primiparous and 16 multiparous) from 3 different farms were obtained and frozen. After thawing, exosomes were isolated following an ultracentrifugation protocol, and characterized morphologically. Particle size distribution and western immunoblotting were also analyzMaed. After RNA extraction, miRNAs were sequenced and analyzed to assess potential differences in profiles between primiparous and multiparous cows from different farms. Fourteen miRNA were upregulated and 11 miRNAs downregulated in primiparous compared with multiparous cows. Most of the miRNA differences between primiparous and multiparous cows regulate the gene expression of factors involved in mammary gland development and differentiation, and lipogenesis. In addition, miRNAs from one of the farms showed 8 miRNAs downregulated and 12 upregulated compared with the other 2 farms, independently of parity. Differences in miRNA between farms were mainly associated with immune and inflammatory-related genes. In conclusion, miRNA cargos of bovine colostrum exosomes differ in primiparous and multiparous cows, and some on-farm practices might also determine the content and activity of miRNA in colostrum exosomes. The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This research was supported by the Spanish Ministry of Economy and Competitivity and FEDER (PGC2018-098847-B-100). IRTA thanks the funding support of the CERCA Program and the Consolidated Research Groups of Sustainable Animal Husbandry (ref. 2021 SGR 01552), both from the Generalitat de Catalunya. info:eu-repo/semantics/publishedVersion Frontiers Media http://hdl.handle.net/20.500.12327/3416 https://doi.org/10.3389/fvets.2024.1463342 |
| spellingShingle | Micro RNA profiles in colostrum exosomes obtained from primiparous or multiparous dairy cows Terre, Marta 636 |
| title | Micro RNA profiles in colostrum exosomes obtained from primiparous or multiparous dairy cows |
| title_full | Micro RNA profiles in colostrum exosomes obtained from primiparous or multiparous dairy cows |
| title_fullStr | Micro RNA profiles in colostrum exosomes obtained from primiparous or multiparous dairy cows |
| title_full_unstemmed | Micro RNA profiles in colostrum exosomes obtained from primiparous or multiparous dairy cows |
| title_short | Micro RNA profiles in colostrum exosomes obtained from primiparous or multiparous dairy cows |
| title_sort | Micro RNA profiles in colostrum exosomes obtained from primiparous or multiparous dairy cows |
| topic | 636 |
| topic_facet | 636 |
| url | http://hdl.handle.net/20.500.12327/3416 https://doi.org/10.3389/fvets.2024.1463342 |