Resequencing theVrs1 gene in Spanish barley landraces revealed reversion of six-rowed to two-rowed spike
Six-rowed spike 1 (Vrs1) is a gene of major importance for barley breeding and germplasm management as it is the main gene determining spike row-type (2-rowed vs. 6-rowed). This is a widely used DUS trait, and has been often associated to phenotypic traits beyond spike type. Comprehensive re-sequenc...
| Authors: | ; ; ; ; ; ; ; ; |
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| Format: | Article |
| Publication date: | 2018 |
| Country: | España |
| Institution: | Institut de Recerca i Tecnologia Agroalimentàries (IRTA) |
| Repository: | IRTA Pubpro. Open Digital Archive |
| OAI Identifier: | oai:repositori.irta.cat:20.500.12327/412 |
| Online Access: | http://hdl.handle.net/20.500.12327/412 https://doi.org/10.1007/s11032-018-0816-z |
| Access Level: | Open access |
| Keyword: | 633 |
| _version_ | 1878733952831717376 |
|---|---|
| author | Casas, Ana M. |
| author2 | Contreras-Moreira, Bruno Cantalapiedra, Carlos P. Sakuma, Shun Gracia, María Pilar Moralejo, Marian Molina-Cano, José Luis Komatsuda, Takao Igartua, Ernesto |
| author2_role | author author author author author author author author |
| author_browse | Cantalapiedra, Carlos P. Casas, Ana M. Contreras-Moreira, Bruno Gracia, María Pilar Igartua, Ernesto Komatsuda, Takao Molina-Cano, José Luis Moralejo, Marian Sakuma, Shun |
| author_facet | Casas, Ana M. Contreras-Moreira, Bruno Cantalapiedra, Carlos P. Sakuma, Shun Gracia, María Pilar Moralejo, Marian Molina-Cano, José Luis Komatsuda, Takao Igartua, Ernesto |
| author_role | author |
| collection | IRTA Pubpro. Open Digital Archive |
| description | Six-rowed spike 1 (Vrs1) is a gene of major importance for barley breeding and germplasm management as it is the main gene determining spike row-type (2-rowed vs. 6-rowed). This is a widely used DUS trait, and has been often associated to phenotypic traits beyond spike type. Comprehensive re-sequencing Vrs1 revealed three two-rowed alleles (Vrs1.b2; Vrs1.b3; Vrs1.t1) and four six-rowed (vrs1.a1; vrs1.a2; vrs1.a3; vrs1.a4) in the natural population. However, the current knowledge about Vrs1 alleles and its distribution among Spanish barley subpopulations is still underexploited. We analyzed the gene in a panel of 215 genotypes, made of Spanish landraces and European cultivars. Among 143 six-rowed accessions, 57 had the vrs1.a1 allele, 83 were vrs1.a2, and three showed the vrs1.a3 allele. Vrs1.b3 was found in most two-rowed accessions, and a new allele was observed in 7 out of 50 two-rowed Spanish landraces. This allele, named Vrs1.b5, contains a ‘T’ insertion in exon 2, originally proposed as the causal mutation giving rise to the six-row vrs1.a2 allele, but has an additional upstream deletion that results in the change of 15 amino acids and a potentially functional protein. We conclude that eight Vrs1 alleles (Vrs1.b2, Vrs1.b3, Vrs1.b5, Vrs1.t1, vrs1.a1, vrs1.a2, vrs1.a3, vrs1.a4) discriminate two and six-rowed barleys. The markers described will be useful for DUS identification, plant breeders, and other crop scientists. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_603db2e72d0dfd632bffe3b70b9a8aae |
| institution | Institut de Recerca i Tecnologia Agroalimentàries (IRTA) |
| instname_str | Institut de Recerca i Tecnologia Agroalimentàries (IRTA) |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:repositori.irta.cat:20.500.12327/412 |
| publishDate | 2018 |
| publishDateSort | 2018 |
| publisher | Springer |
| publisherStr | Springer |
| reponame_str | IRTA Pubpro. Open Digital Archive |
| spelling | Resequencing theVrs1 gene in Spanish barley landraces revealed reversion of six-rowed to two-rowed spike Casas, Ana M. Contreras-Moreira, Bruno Cantalapiedra, Carlos P. Sakuma, Shun Gracia, María Pilar Moralejo, Marian Molina-Cano, José Luis Komatsuda, Takao Igartua, Ernesto 633 Six-rowed spike 1 (Vrs1) is a gene of major importance for barley breeding and germplasm management as it is the main gene determining spike row-type (2-rowed vs. 6-rowed). This is a widely used DUS trait, and has been often associated to phenotypic traits beyond spike type. Comprehensive re-sequencing Vrs1 revealed three two-rowed alleles (Vrs1.b2; Vrs1.b3; Vrs1.t1) and four six-rowed (vrs1.a1; vrs1.a2; vrs1.a3; vrs1.a4) in the natural population. However, the current knowledge about Vrs1 alleles and its distribution among Spanish barley subpopulations is still underexploited. We analyzed the gene in a panel of 215 genotypes, made of Spanish landraces and European cultivars. Among 143 six-rowed accessions, 57 had the vrs1.a1 allele, 83 were vrs1.a2, and three showed the vrs1.a3 allele. Vrs1.b3 was found in most two-rowed accessions, and a new allele was observed in 7 out of 50 two-rowed Spanish landraces. This allele, named Vrs1.b5, contains a ‘T’ insertion in exon 2, originally proposed as the causal mutation giving rise to the six-row vrs1.a2 allele, but has an additional upstream deletion that results in the change of 15 amino acids and a potentially functional protein. We conclude that eight Vrs1 alleles (Vrs1.b2, Vrs1.b3, Vrs1.b5, Vrs1.t1, vrs1.a1, vrs1.a2, vrs1.a3, vrs1.a4) discriminate two and six-rowed barleys. The markers described will be useful for DUS identification, plant breeders, and other crop scientists. info:eu-repo/semantics/acceptedVersion Springer http://hdl.handle.net/20.500.12327/412 https://doi.org/10.1007/s11032-018-0816-z |
| spellingShingle | Resequencing theVrs1 gene in Spanish barley landraces revealed reversion of six-rowed to two-rowed spike Casas, Ana M. 633 |
| title | Resequencing theVrs1 gene in Spanish barley landraces revealed reversion of six-rowed to two-rowed spike |
| title_full | Resequencing theVrs1 gene in Spanish barley landraces revealed reversion of six-rowed to two-rowed spike |
| title_fullStr | Resequencing theVrs1 gene in Spanish barley landraces revealed reversion of six-rowed to two-rowed spike |
| title_full_unstemmed | Resequencing theVrs1 gene in Spanish barley landraces revealed reversion of six-rowed to two-rowed spike |
| title_short | Resequencing theVrs1 gene in Spanish barley landraces revealed reversion of six-rowed to two-rowed spike |
| title_sort | Resequencing theVrs1 gene in Spanish barley landraces revealed reversion of six-rowed to two-rowed spike |
| topic | 633 |
| topic_facet | 633 |
| url | http://hdl.handle.net/20.500.12327/412 https://doi.org/10.1007/s11032-018-0816-z |