Novel Targets Regulating the Role of Endothelial Cells and Angiogenesis after Infarction: A RNA Sequencing Analysis
Endothelial cells (ECs) are a key target for cardioprotection due to their role in preserving cardiac microvasculature and homeostasis after myocardial infarction (MI). Our goal is to identify the genes involved in post-MI EC proliferation, EC apoptosis, and angiogenesis regulation via RNA-sequencin...
| Authors: | ; ; ; ; ; ; ; ; ; |
|---|---|
| Format: | Article |
| Status: | Published version |
| Publication date: | 2023 |
| Country: | España |
| Institution: | INCLIVA |
| Repository: | r-INCLIVA. Repositorio Institucional de Producción Científica de INCLIVA |
| OAI Identifier: | oai:incliva.fundanetsuite.com:p17814 |
| Online Access: | https://incliva.portalinvestigacion.com/publicaciones/17814 |
| Access Level: | Open access |
| Keyword: | myocardial infarction angiogenesis endothelial cells RNA sequencing |
| _version_ | 1878733699418161152 |
|---|---|
| author | Ortega, Maria |
| author2 | Molina-Garcia, Tamara Gavara, Jose de Dios, Elena Perez-Sole, Nerea Marcos-Garces, Victor Chorro, Francisco J Rios-Navarro, Cesar Ruiz-Sauri, Amparo Bodi, Vicente |
| author2_role | author author author author author author author author author |
| author_browse | Bodi, Vicente Chorro, Francisco J Gavara, Jose Marcos-Garces, Victor Molina-Garcia, Tamara Ortega, Maria Perez-Sole, Nerea Rios-Navarro, Cesar Ruiz-Sauri, Amparo de Dios, Elena |
| author_facet | Ortega, Maria Molina-Garcia, Tamara Gavara, Jose de Dios, Elena Perez-Sole, Nerea Marcos-Garces, Victor Chorro, Francisco J Rios-Navarro, Cesar Ruiz-Sauri, Amparo Bodi, Vicente |
| author_role | author |
| collection | r-INCLIVA. Repositorio Institucional de Producción Científica de INCLIVA |
| description | Endothelial cells (ECs) are a key target for cardioprotection due to their role in preserving cardiac microvasculature and homeostasis after myocardial infarction (MI). Our goal is to identify the genes involved in post-MI EC proliferation, EC apoptosis, and angiogenesis regulation via RNA-sequencing transcriptomic datasets. Using eight studies from the Gene Expression Omnibus, RNA-sequencing data from 92 mice submitted to different times of coronary ischemia or sham were chosen. Functional enrichment analysis was performed based on gene ontology biological processes (BPs). Apoptosis-related BPs are activated up to day 3 after ischemia onset, whereas endothelial proliferation occurs from day 3 onwards, including an overrepresentation of up to 37 genes. Endothelial apoptosis post-MI is triggered via both the extrinsic and intrinsic signaling pathways, as reflected by the overrepresentation of 13 and 2 specific genes, respectively. BPs implicated in new vessel formation are upregulated soon after ischemia onset, whilst the mechanisms aiming at angiogenesis repression can be detected at day 3. Overall, 51 pro-angiogenic and 29 anti-angiogenic factors displayed altered transcriptomic expression post-MI. This is the first study using RNA sequencing datasets to evaluate the genes participating in post-MI endothelium physiology and angiogenesis regulation. These novel data could lay the groundwork to advance understanding of the implication of ECs after MI. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_5b47ec0bed2c9f8c5edea1ec3cc4e14c |
| institution | INCLIVA |
| instname_str | INCLIVA |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:incliva.fundanetsuite.com:p17814 |
| publishDate | 2023 |
| publishDateSort | 2023 |
| publisher | MDPI |
| publisherStr | MDPI |
| reponame_str | r-INCLIVA. Repositorio Institucional de Producción Científica de INCLIVA |
| spelling | Novel Targets Regulating the Role of Endothelial Cells and Angiogenesis after Infarction: A RNA Sequencing Analysis Ortega, Maria Molina-Garcia, Tamara Gavara, Jose de Dios, Elena Perez-Sole, Nerea Marcos-Garces, Victor Chorro, Francisco J Rios-Navarro, Cesar Ruiz-Sauri, Amparo Bodi, Vicente myocardial infarction angiogenesis endothelial cells RNA sequencing Endothelial cells (ECs) are a key target for cardioprotection due to their role in preserving cardiac microvasculature and homeostasis after myocardial infarction (MI). Our goal is to identify the genes involved in post-MI EC proliferation, EC apoptosis, and angiogenesis regulation via RNA-sequencing transcriptomic datasets. Using eight studies from the Gene Expression Omnibus, RNA-sequencing data from 92 mice submitted to different times of coronary ischemia or sham were chosen. Functional enrichment analysis was performed based on gene ontology biological processes (BPs). Apoptosis-related BPs are activated up to day 3 after ischemia onset, whereas endothelial proliferation occurs from day 3 onwards, including an overrepresentation of up to 37 genes. Endothelial apoptosis post-MI is triggered via both the extrinsic and intrinsic signaling pathways, as reflected by the overrepresentation of 13 and 2 specific genes, respectively. BPs implicated in new vessel formation are upregulated soon after ischemia onset, whilst the mechanisms aiming at angiogenesis repression can be detected at day 3. Overall, 51 pro-angiogenic and 29 anti-angiogenic factors displayed altered transcriptomic expression post-MI. This is the first study using RNA sequencing datasets to evaluate the genes participating in post-MI endothelium physiology and angiogenesis regulation. These novel data could lay the groundwork to advance understanding of the implication of ECs after MI. MDPI https://incliva.portalinvestigacion.com/publicaciones/17814 |
| spellingShingle | Novel Targets Regulating the Role of Endothelial Cells and Angiogenesis after Infarction: A RNA Sequencing Analysis Ortega, Maria myocardial infarction angiogenesis endothelial cells RNA sequencing |
| status_str | publishedVersion |
| title | Novel Targets Regulating the Role of Endothelial Cells and Angiogenesis after Infarction: A RNA Sequencing Analysis |
| title_full | Novel Targets Regulating the Role of Endothelial Cells and Angiogenesis after Infarction: A RNA Sequencing Analysis |
| title_fullStr | Novel Targets Regulating the Role of Endothelial Cells and Angiogenesis after Infarction: A RNA Sequencing Analysis |
| title_full_unstemmed | Novel Targets Regulating the Role of Endothelial Cells and Angiogenesis after Infarction: A RNA Sequencing Analysis |
| title_short | Novel Targets Regulating the Role of Endothelial Cells and Angiogenesis after Infarction: A RNA Sequencing Analysis |
| title_sort | Novel Targets Regulating the Role of Endothelial Cells and Angiogenesis after Infarction: A RNA Sequencing Analysis |
| topic | myocardial infarction angiogenesis endothelial cells RNA sequencing |
| topic_facet | myocardial infarction angiogenesis endothelial cells RNA sequencing |
| url | https://incliva.portalinvestigacion.com/publicaciones/17814 |