A label-free nanostructured plasmonic biosensor based on Blu-ray discs with integrated microfluidics for sensitive biodetection
Nanostructure-based plasmonic biosensors have quickly positioned themselves as interesting candidates for the design of portable optical biosensor platforms considering the potential benefits they can offer in integration, miniaturization, multiplexing, and real-time label-free detection. We have de...
| Authors: | ; ; ; ; ; ; |
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| Format: | Article |
| Publication date: | 2017 |
| Country: | España |
| Institution: | Universitat Autònoma de Barcelona |
| Repository: | Dipòsit Digital de Documents de la UAB |
| Language: | English |
| OAI Identifier: | oai:ddd.uab.cat:211125 |
| Online Access: | https://ddd.uab.cat/record/211125 https://dx.doi.org/urn:doi:10.1016/j.bios.2017.05.020 |
| Access Level: | Open access |
| Keyword: | Blu-ray disc Plasmonic nanostructure Integration Microfluidics Biosensing Antibody |
| _version_ | 1878733041227005953 |
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| author | López Muñoz, Gerardo Arturo |
| author2 | Estévez, M. Carmen|||0000-0003-3694-7186 Peláez Gutiérrez, Enelia Cristina|||0000-0002-9482-4566 Homs Corbera, Antoni García-Hernandez, M. C. Imbaud, J. I. Lechuga, Laura M.|||0000-0001-5187-5358 |
| author2_role | author author author author author author |
| author_browse | Estévez, M. Carmen|||0000-0003-3694-7186 García-Hernandez, M. C. Homs Corbera, Antoni Imbaud, J. I. Lechuga, Laura M.|||0000-0001-5187-5358 López Muñoz, Gerardo Arturo Peláez Gutiérrez, Enelia Cristina|||0000-0002-9482-4566 |
| author_facet | López Muñoz, Gerardo Arturo Estévez, M. Carmen|||0000-0003-3694-7186 Peláez Gutiérrez, Enelia Cristina|||0000-0002-9482-4566 Homs Corbera, Antoni García-Hernandez, M. C. Imbaud, J. I. Lechuga, Laura M.|||0000-0001-5187-5358 |
| author_role | author |
| collection | Dipòsit Digital de Documents de la UAB |
| description | Nanostructure-based plasmonic biosensors have quickly positioned themselves as interesting candidates for the design of portable optical biosensor platforms considering the potential benefits they can offer in integration, miniaturization, multiplexing, and real-time label-free detection. We have developed a simple integrated nanoplasmonic sensor taking advantage of the periodic nanostructured array of commercial Blu-ray discs. Sensors with two gold film thicknesses (50 and 100 nm) were fabricated and optically characterized by varying the oblique-angle of the incident light in optical reflectance measurements. Contrary to the use normal light incidence previously reported with other optical discs, we observed an enhancement in sensitivity and a narrowing of the resonant linewidths as the light incidence angle was increased, which could be related to the generation of Fano resonant modes. The new sensors achieve a figure of merit (FOM) up to 35 RIU and a competitive bulk limit of detection (LOD) of 6.3×10 RIU. These values significantly improve previously reported results obtained with normal light incidence reflectance measurements using similar structures. The sensor has been combined with versatile, simple, ease to-fabricate microfluidics. The integrated chip is only 1 cm (including a PDMS flow cell with a 50 µm height microfluidic channel fabricated with double-sided adhesive tape) and all the optical components are mounted on a 10 cm×10 cm portable prototype, illustrating its facile miniaturization, integration and potential portability. Finally, to assess the label-free biosensing capability of the new sensor, we have evaluated the presence of specific antibodies against the GTF2b protein, a tumor-associate antigen (TAA) related to colorectal cancer. We have achieved a LOD in the pM order and have assessed the feasibility of directly measuring biological samples such as human serum. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_4eee0004e1b6070a2e1ffe94f86124e9 |
| institution | Universitat Autònoma de Barcelona |
| instname_str | Universitat Autònoma de Barcelona |
| language | eng |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:ddd.uab.cat:211125 |
| publishDate | 2017 |
| publishDateSort | 2017 |
| reponame_str | Dipòsit Digital de Documents de la UAB |
| spelling | A label-free nanostructured plasmonic biosensor based on Blu-ray discs with integrated microfluidics for sensitive biodetection López Muñoz, Gerardo Arturo Estévez, M. Carmen|||0000-0003-3694-7186 Peláez Gutiérrez, Enelia Cristina|||0000-0002-9482-4566 Homs Corbera, Antoni García-Hernandez, M. C. Imbaud, J. I. Lechuga, Laura M.|||0000-0001-5187-5358 Blu-ray disc Plasmonic nanostructure Integration Microfluidics Biosensing Antibody Nanostructure-based plasmonic biosensors have quickly positioned themselves as interesting candidates for the design of portable optical biosensor platforms considering the potential benefits they can offer in integration, miniaturization, multiplexing, and real-time label-free detection. We have developed a simple integrated nanoplasmonic sensor taking advantage of the periodic nanostructured array of commercial Blu-ray discs. Sensors with two gold film thicknesses (50 and 100 nm) were fabricated and optically characterized by varying the oblique-angle of the incident light in optical reflectance measurements. Contrary to the use normal light incidence previously reported with other optical discs, we observed an enhancement in sensitivity and a narrowing of the resonant linewidths as the light incidence angle was increased, which could be related to the generation of Fano resonant modes. The new sensors achieve a figure of merit (FOM) up to 35 RIU and a competitive bulk limit of detection (LOD) of 6.3×10 RIU. These values significantly improve previously reported results obtained with normal light incidence reflectance measurements using similar structures. The sensor has been combined with versatile, simple, ease to-fabricate microfluidics. The integrated chip is only 1 cm (including a PDMS flow cell with a 50 µm height microfluidic channel fabricated with double-sided adhesive tape) and all the optical components are mounted on a 10 cm×10 cm portable prototype, illustrating its facile miniaturization, integration and potential portability. Finally, to assess the label-free biosensing capability of the new sensor, we have evaluated the presence of specific antibodies against the GTF2b protein, a tumor-associate antigen (TAA) related to colorectal cancer. We have achieved a LOD in the pM order and have assessed the feasibility of directly measuring biological samples such as human serum. https://ddd.uab.cat/record/211125 https://dx.doi.org/urn:doi:10.1016/j.bios.2017.05.020 |
| spellingShingle | A label-free nanostructured plasmonic biosensor based on Blu-ray discs with integrated microfluidics for sensitive biodetection López Muñoz, Gerardo Arturo Blu-ray disc Plasmonic nanostructure Integration Microfluidics Biosensing Antibody |
| title | A label-free nanostructured plasmonic biosensor based on Blu-ray discs with integrated microfluidics for sensitive biodetection |
| title_full | A label-free nanostructured plasmonic biosensor based on Blu-ray discs with integrated microfluidics for sensitive biodetection |
| title_fullStr | A label-free nanostructured plasmonic biosensor based on Blu-ray discs with integrated microfluidics for sensitive biodetection |
| title_full_unstemmed | A label-free nanostructured plasmonic biosensor based on Blu-ray discs with integrated microfluidics for sensitive biodetection |
| title_short | A label-free nanostructured plasmonic biosensor based on Blu-ray discs with integrated microfluidics for sensitive biodetection |
| title_sort | A label-free nanostructured plasmonic biosensor based on Blu-ray discs with integrated microfluidics for sensitive biodetection |
| topic | Blu-ray disc Plasmonic nanostructure Integration Microfluidics Biosensing Antibody |
| topic_facet | Blu-ray disc Plasmonic nanostructure Integration Microfluidics Biosensing Antibody |
| url | https://ddd.uab.cat/record/211125 https://dx.doi.org/urn:doi:10.1016/j.bios.2017.05.020 |