Identification of a newly conserved SLA-II epitope in a structural protein of swine influenza virus
Despite the role of pigs as a source of new Influenza A Virus viruses (IAV) potentially capable of initiating human pandemics, immune responses to swine influenza virus (SwIV) in pigs are not fully understood. Several SwIV epitopes presented by swine MHC (SLA) class I have been identified using diff...
| Authors: | ; ; ; ; ; ; ; ; ; ; |
|---|---|
| Format: | Article |
| Status: | Published version |
| Publication date: | 2020 |
| Country: | España |
| Institution: | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| Repository: | Recercat. Dipósit de la Recerca de Catalunya |
| OAI Identifier: | oai:recercat.cat:10459.1/69571 |
| Online Access: | https://doi.org/10.3389/fimmu.2020.02083 http://hdl.handle.net/10459.1/69571 |
| Access Level: | Open access |
| Keyword: | Swine influenza Class II epitopes Swine Nucleoprotein |
| _version_ | 1878732987927887872 |
|---|---|
| author | Baratelli, Massimiliano |
| author2 | Morgan, Sophie Hemmink, Johanneke Dinie Reid, Elisabeth Carr, Brigid Veronica Lefevre, Eric Montaner Tarbes, Sergio Roberto Charleston, Bryan Fraile Sauce, Lorenzo José Tchilian, Elma Montoya, Maria |
| author2_role | author author author author author author author author author author |
| author_browse | Baratelli, Massimiliano Carr, Brigid Veronica Charleston, Bryan Fraile Sauce, Lorenzo José Hemmink, Johanneke Dinie Lefevre, Eric Montaner Tarbes, Sergio Roberto Montoya, Maria Morgan, Sophie Reid, Elisabeth Tchilian, Elma |
| author_facet | Baratelli, Massimiliano Morgan, Sophie Hemmink, Johanneke Dinie Reid, Elisabeth Carr, Brigid Veronica Lefevre, Eric Montaner Tarbes, Sergio Roberto Charleston, Bryan Fraile Sauce, Lorenzo José Tchilian, Elma Montoya, Maria |
| author_role | author |
| collection | Recercat. Dipósit de la Recerca de Catalunya |
| description | Despite the role of pigs as a source of new Influenza A Virus viruses (IAV) potentially capable of initiating human pandemics, immune responses to swine influenza virus (SwIV) in pigs are not fully understood. Several SwIV epitopes presented by swine MHC (SLA) class I have been identified using different approaches either in outbred pigs or in Babraham large white inbred pigs, which are 85% identical by genome wide SNP analysis. On the other hand, some class II SLA epitopes were recently described in outbred pigs. In this work, Babraham large white inbred pigs were selected to identify SLA II epitopes from SwIV H1N1. PBMCs were screened for recognition of overlapping peptides covering the NP and M1 proteins from heterologous IAV H1N1 in IFNγ ELISPOT. A novel SLA class II restricted epitope was identified in NP from swine H1N1. This conserved novel epitope could be the base for further vaccine approaches against H1N1 in pigs. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_4ddbca5cefb36f8eaa5e2c06eb79b4ef |
| institution | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| instname_str | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:recercat.cat:10459.1/69571 |
| publishDate | 2020 |
| publishDateSort | 2020 |
| publisher | Frontiers Media |
| publisherStr | Frontiers Media |
| reponame_str | Recercat. Dipósit de la Recerca de Catalunya |
| spelling | Identification of a newly conserved SLA-II epitope in a structural protein of swine influenza virus Baratelli, Massimiliano Morgan, Sophie Hemmink, Johanneke Dinie Reid, Elisabeth Carr, Brigid Veronica Lefevre, Eric Montaner Tarbes, Sergio Roberto Charleston, Bryan Fraile Sauce, Lorenzo José Tchilian, Elma Montoya, Maria Swine influenza Class II epitopes Swine Nucleoprotein Despite the role of pigs as a source of new Influenza A Virus viruses (IAV) potentially capable of initiating human pandemics, immune responses to swine influenza virus (SwIV) in pigs are not fully understood. Several SwIV epitopes presented by swine MHC (SLA) class I have been identified using different approaches either in outbred pigs or in Babraham large white inbred pigs, which are 85% identical by genome wide SNP analysis. On the other hand, some class II SLA epitopes were recently described in outbred pigs. In this work, Babraham large white inbred pigs were selected to identify SLA II epitopes from SwIV H1N1. PBMCs were screened for recognition of overlapping peptides covering the NP and M1 proteins from heterologous IAV H1N1 in IFNγ ELISPOT. A novel SLA class II restricted epitope was identified in NP from swine H1N1. This conserved novel epitope could be the base for further vaccine approaches against H1N1 in pigs. The research leading to these results has received funding from the European Community's Seventh Framework Program (FP7, 2007-2013), the Research Infrastructures Action under grant FP7-228393 (a NADIR project); from the project AGL2010-22200-C02-01 from the Spanish Ministry of Science and Innovation; the UK's BBSRC grant BBS/E/I/00002014. Frontiers Media https://doi.org/10.3389/fimmu.2020.02083 http://hdl.handle.net/10459.1/69571 |
| spellingShingle | Identification of a newly conserved SLA-II epitope in a structural protein of swine influenza virus Baratelli, Massimiliano Swine influenza Class II epitopes Swine Nucleoprotein |
| status_str | publishedVersion |
| title | Identification of a newly conserved SLA-II epitope in a structural protein of swine influenza virus |
| title_full | Identification of a newly conserved SLA-II epitope in a structural protein of swine influenza virus |
| title_fullStr | Identification of a newly conserved SLA-II epitope in a structural protein of swine influenza virus |
| title_full_unstemmed | Identification of a newly conserved SLA-II epitope in a structural protein of swine influenza virus |
| title_short | Identification of a newly conserved SLA-II epitope in a structural protein of swine influenza virus |
| title_sort | Identification of a newly conserved SLA-II epitope in a structural protein of swine influenza virus |
| topic | Swine influenza Class II epitopes Swine Nucleoprotein |
| topic_facet | Swine influenza Class II epitopes Swine Nucleoprotein |
| url | https://doi.org/10.3389/fimmu.2020.02083 http://hdl.handle.net/10459.1/69571 |