Epicardial cell shape and maturation are regulated by Wt1 via transcriptional control of Bmp4
The epicardium plays a crucial role in embryonic heart development and adult heart repair; however, the molecular events underlying its maturation remain unknown. Wt1, one of the main markers of the embryonic epicardium, is essential for epicardial development and function. Here, we analyse the tran...
| Autores: | ; ; ; ; ; ; ; ; ; ; ; ; |
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| Tipo de documento: | Artículo |
| Estado: | Published version |
| Fecha de publicación: | 2019 |
| País: | España |
| Institución: | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| Repositorio: | Recercat. Dipósit de la Recerca de Catalunya |
| OAI Identifier: | oai:recercat.cat:2445/175561 |
| Acceso en línea: | https://hdl.handle.net/2445/175561 |
| Nivel de acceso: | Acceso abierto |
| Palabra clave: | Cèl·lules epitelials Epithelial cells |
| _version_ | 1878732961771159552 |
|---|---|
| author | Velecela, Victor |
| author2 | Torres Cano, Alejo García Melero, Ana Ramiro-Pareta, Marina Müller Sánchez, Claudia Alejandra Segarra Mondéjar, Marc Chau, You-Ying Campos Bonilla, Begoña Reina del Pozo, Manuel Soriano Zaragoza, Francesc X. (Francesc Xavier) Hastie, Nicholas D. Martinez, Fernando O. Martínez Estrada, Ofelia María |
| author2_role | author author author author author author author author author author author author |
| author_browse | Campos Bonilla, Begoña Chau, You-Ying García Melero, Ana Hastie, Nicholas D. Martinez, Fernando O. Martínez Estrada, Ofelia María Müller Sánchez, Claudia Alejandra Ramiro-Pareta, Marina Reina del Pozo, Manuel Segarra Mondéjar, Marc Soriano Zaragoza, Francesc X. (Francesc Xavier) Torres Cano, Alejo Velecela, Victor |
| author_facet | Velecela, Victor Torres Cano, Alejo García Melero, Ana Ramiro-Pareta, Marina Müller Sánchez, Claudia Alejandra Segarra Mondéjar, Marc Chau, You-Ying Campos Bonilla, Begoña Reina del Pozo, Manuel Soriano Zaragoza, Francesc X. (Francesc Xavier) Hastie, Nicholas D. Martinez, Fernando O. Martínez Estrada, Ofelia María |
| author_role | author |
| collection | Recercat. Dipósit de la Recerca de Catalunya |
| description | The epicardium plays a crucial role in embryonic heart development and adult heart repair; however, the molecular events underlying its maturation remain unknown. Wt1, one of the main markers of the embryonic epicardium, is essential for epicardial development and function. Here, we analyse the transcriptomic profile of epicardial-enriched cells at different stages of development and from control and epicardial-specific Wt1 knockout (Wt1KO) mice. Transcriptomic and cell morphology analyses of epicardial cells from epicardial-specific Wt1KO mice revealed a defect in the maturation process of the mutant epicardium, including sustained upregulation of Bmp4 expression and the inability of mutant epicardial cells to transition into a mature squamous phenotype. We identified Bmp4 as a transcriptional target of Wt1, thus providing a molecular basis for the retention of the cuboidal cell shape observed in the Wt1KO epicardium. Accordingly, inhibition of the Bmp4 signalling pathway both ex vivo and in vivo rescued the cuboidal phenotype of the mutant epicardium. Our findings indicate the importance of the cuboidal-to-squamous transition in epicardial maturation, a process regulated by Wt1. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_4d5541a2ce4a2d6bf8e9fb2f965ef4bb |
| institution | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| instname_str | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:recercat.cat:2445/175561 |
| publishDate | 2019 |
| publishDateSort | 2019 |
| publisher | The Company of Biologists |
| publisherStr | The Company of Biologists |
| reponame_str | Recercat. Dipósit de la Recerca de Catalunya |
| spelling | Epicardial cell shape and maturation are regulated by Wt1 via transcriptional control of Bmp4 Velecela, Victor Torres Cano, Alejo García Melero, Ana Ramiro-Pareta, Marina Müller Sánchez, Claudia Alejandra Segarra Mondéjar, Marc Chau, You-Ying Campos Bonilla, Begoña Reina del Pozo, Manuel Soriano Zaragoza, Francesc X. (Francesc Xavier) Hastie, Nicholas D. Martinez, Fernando O. Martínez Estrada, Ofelia María Cèl·lules epitelials Epithelial cells The epicardium plays a crucial role in embryonic heart development and adult heart repair; however, the molecular events underlying its maturation remain unknown. Wt1, one of the main markers of the embryonic epicardium, is essential for epicardial development and function. Here, we analyse the transcriptomic profile of epicardial-enriched cells at different stages of development and from control and epicardial-specific Wt1 knockout (Wt1KO) mice. Transcriptomic and cell morphology analyses of epicardial cells from epicardial-specific Wt1KO mice revealed a defect in the maturation process of the mutant epicardium, including sustained upregulation of Bmp4 expression and the inability of mutant epicardial cells to transition into a mature squamous phenotype. We identified Bmp4 as a transcriptional target of Wt1, thus providing a molecular basis for the retention of the cuboidal cell shape observed in the Wt1KO epicardium. Accordingly, inhibition of the Bmp4 signalling pathway both ex vivo and in vivo rescued the cuboidal phenotype of the mutant epicardium. Our findings indicate the importance of the cuboidal-to-squamous transition in epicardial maturation, a process regulated by Wt1. The Company of Biologists https://hdl.handle.net/2445/175561 |
| spellingShingle | Epicardial cell shape and maturation are regulated by Wt1 via transcriptional control of Bmp4 Velecela, Victor Cèl·lules epitelials Epithelial cells |
| status_str | publishedVersion |
| title | Epicardial cell shape and maturation are regulated by Wt1 via transcriptional control of Bmp4 |
| title_full | Epicardial cell shape and maturation are regulated by Wt1 via transcriptional control of Bmp4 |
| title_fullStr | Epicardial cell shape and maturation are regulated by Wt1 via transcriptional control of Bmp4 |
| title_full_unstemmed | Epicardial cell shape and maturation are regulated by Wt1 via transcriptional control of Bmp4 |
| title_short | Epicardial cell shape and maturation are regulated by Wt1 via transcriptional control of Bmp4 |
| title_sort | Epicardial cell shape and maturation are regulated by Wt1 via transcriptional control of Bmp4 |
| topic | Cèl·lules epitelials Epithelial cells |
| topic_facet | Cèl·lules epitelials Epithelial cells |
| url | https://hdl.handle.net/2445/175561 |