MicroRNA profiling during mouse ventricular maturation: a role for miR-27 modulating Mef2c expression
Aims: Non-coding RNA has been recently demonstrated to be a novel mechanism for modulation of gene expression at the post-transcriptional level. The importance of microRNAs in the cardiovascular system is now apparent. Mutations of distinct microRNAs have provided evidence for fundamental roles of m...
| Autores: | ; ; ; ; ; ; |
|---|---|
| Tipo de documento: | Artículo |
| Estado: | Published version |
| Fecha de publicación: | 2010 |
| País: | España |
| Institución: | Universidad de Jaén |
| Repositorio: | RUJA. Repositorio Institucional de la Producción Científica de la Universidad de Jaén |
| OAI Identifier: | oai:ruja.ujaen.es:10953/4010 |
| Acceso en línea: | https://academic.oup.com/cardiovascres/article/89/1/98/325183?login=true https://hdl.handle.net/10953/4010 |
| Nivel de acceso: | Acceso abierto |
| Palabra clave: | microRNAs Cardiogenesis Microarrays Mef2c miR-27b |
| _version_ | 1878732895902760960 |
|---|---|
| author | Chinchilla, Ana |
| author2 | Lozano-Velasco, Estefanía Daimi, Houria Esteban-Ruiz, Francisco José Crist, Colin Aránega, Amelia Eva Franco, Diego |
| author2_role | author author author author author author |
| author_browse | Aránega, Amelia Eva Chinchilla, Ana Crist, Colin Daimi, Houria Esteban-Ruiz, Francisco José Franco, Diego Lozano-Velasco, Estefanía |
| author_facet | Chinchilla, Ana Lozano-Velasco, Estefanía Daimi, Houria Esteban-Ruiz, Francisco José Crist, Colin Aránega, Amelia Eva Franco, Diego |
| author_role | author |
| collection | RUJA. Repositorio Institucional de la Producción Científica de la Universidad de Jaén |
| description | Aims: Non-coding RNA has been recently demonstrated to be a novel mechanism for modulation of gene expression at the post-transcriptional level. The importance of microRNAs in the cardiovascular system is now apparent. Mutations of distinct microRNAs have provided evidence for fundamental roles of microRNAs during cardiovascular development. However, there is limited information about global microRNA profiles during mouse heart development. In this study, we have gained insight from the expression profiles of microRNAs during mouse ventricular development by microarray and qRT-PCR analysis. Methods and results: Our microarray analysis reveals that relatively few microRNAs display either increasing or decreasing expression profiles during ventricular chamber formation. Interestingly, most of the differentially expressed microRNAs display a rather discrete peak of expression at particular developmental stages. Furthermore, we demonstrate that micro-RNA-27b (miR-27b) displays an overt myocardial expression during heart development and that the transcription factor-encoding gene Mef2c is an miR-27b target. Conclusion: Our data present a comprehensive profile of microRNA expression during ventricular maturation, providing an entry point for investigation of the functional roles of the most abundantly and differentially expressed microRNAs during cardiogenesis |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_4bf9dc0b6c376bb155243dc7a1d3c5ce |
| institution | Universidad de Jaén |
| instname_str | Universidad de Jaén |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:ruja.ujaen.es:10953/4010 |
| publishDate | 2010 |
| publishDateSort | 2010 |
| publisher | Oxford |
| publisherStr | Oxford |
| reponame_str | RUJA. Repositorio Institucional de la Producción Científica de la Universidad de Jaén |
| spelling | MicroRNA profiling during mouse ventricular maturation: a role for miR-27 modulating Mef2c expression Chinchilla, Ana Lozano-Velasco, Estefanía Daimi, Houria Esteban-Ruiz, Francisco José Crist, Colin Aránega, Amelia Eva Franco, Diego microRNAs Cardiogenesis Microarrays Mef2c miR-27b Aims: Non-coding RNA has been recently demonstrated to be a novel mechanism for modulation of gene expression at the post-transcriptional level. The importance of microRNAs in the cardiovascular system is now apparent. Mutations of distinct microRNAs have provided evidence for fundamental roles of microRNAs during cardiovascular development. However, there is limited information about global microRNA profiles during mouse heart development. In this study, we have gained insight from the expression profiles of microRNAs during mouse ventricular development by microarray and qRT-PCR analysis. Methods and results: Our microarray analysis reveals that relatively few microRNAs display either increasing or decreasing expression profiles during ventricular chamber formation. Interestingly, most of the differentially expressed microRNAs display a rather discrete peak of expression at particular developmental stages. Furthermore, we demonstrate that micro-RNA-27b (miR-27b) displays an overt myocardial expression during heart development and that the transcription factor-encoding gene Mef2c is an miR-27b target. Conclusion: Our data present a comprehensive profile of microRNA expression during ventricular maturation, providing an entry point for investigation of the functional roles of the most abundantly and differentially expressed microRNAs during cardiogenesis VI EU Integrated Project ‘Heart Failure and Cardiac Repair’ LSHM-CT-2005-018630; Junta de Andalucía Proyecto de Excelencia ‘Desarrollo Cardiovascular y Terapia Celular’ CTS-1614; Ministerio de Ciencia e Innovación MICINN BFU2009-11566 y MICINN BFU2008-01217; Junta de Andalucia CTS-446 y BIO-302 Oxford https://academic.oup.com/cardiovascres/article/89/1/98/325183?login=true https://hdl.handle.net/10953/4010 |
| spellingShingle | MicroRNA profiling during mouse ventricular maturation: a role for miR-27 modulating Mef2c expression Chinchilla, Ana microRNAs Cardiogenesis Microarrays Mef2c miR-27b |
| status_str | publishedVersion |
| title | MicroRNA profiling during mouse ventricular maturation: a role for miR-27 modulating Mef2c expression |
| title_full | MicroRNA profiling during mouse ventricular maturation: a role for miR-27 modulating Mef2c expression |
| title_fullStr | MicroRNA profiling during mouse ventricular maturation: a role for miR-27 modulating Mef2c expression |
| title_full_unstemmed | MicroRNA profiling during mouse ventricular maturation: a role for miR-27 modulating Mef2c expression |
| title_short | MicroRNA profiling during mouse ventricular maturation: a role for miR-27 modulating Mef2c expression |
| title_sort | MicroRNA profiling during mouse ventricular maturation: a role for miR-27 modulating Mef2c expression |
| topic | microRNAs Cardiogenesis Microarrays Mef2c miR-27b |
| topic_facet | microRNAs Cardiogenesis Microarrays Mef2c miR-27b |
| url | https://academic.oup.com/cardiovascres/article/89/1/98/325183?login=true https://hdl.handle.net/10953/4010 |