Prenatal Administration of Oleic Acid or Linolenic Acid Reduces Neuromorphological and Cognitive Alterations in Ts65dn Down Syndrome Mice
Background: The cognitive impairments that characterize Down syndrome (DS) have been attributed to brain hypocellularity due to neurogenesis impairment during fetal stages. Thus, enhancing prenatal neurogenesis in DS could prevent or reduce some of the neuromorphological and cognitive defects found...
| Autores: | ; ; ; ; ; ; ; ; ; |
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| Tipo de documento: | Artículo |
| Fecha de publicación: | 2020 |
| País: | España |
| Institución: | Universidad de Cantabria (UC) |
| Repositorio: | UCrea Repositorio Abierto de la Universidad de Cantabria |
| Lenguaje: | Inglés |
| OAI Identifier: | oai:repositorio.unican.es:10902/21334 |
| Acceso en línea: | http://hdl.handle.net/10902/21334 |
| Nivel de acceso: | Acceso abierto |
| Palabra clave: | Down Syndrome Ts65Dn Mice Cognition Linolenic Acid Neurogenesis Oleic Acid Prenatal Treatment |
| _version_ | 1878732752107339776 |
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| author | García Cerro, Susana |
| author2 | Rueda Revilla, Noemí|||0000-0001-9583-3336 Vidal Sánchez, Verónica Puente Bedia, Alba Campa Fernández, Víctor Manuel Lantigua Romero, Sara Narcís Majos, Josep Oriol Velasco, Ana Bartesaghi, Renata Martínez-Cué, Carmen|||0000-0002-2137-1156 |
| author2_role | author author author author author author author author author |
| author_browse | Bartesaghi, Renata Campa Fernández, Víctor Manuel García Cerro, Susana Lantigua Romero, Sara Martínez-Cué, Carmen|||0000-0002-2137-1156 Narcís Majos, Josep Oriol Puente Bedia, Alba Rueda Revilla, Noemí|||0000-0001-9583-3336 Velasco, Ana Vidal Sánchez, Verónica |
| author_facet | García Cerro, Susana Rueda Revilla, Noemí|||0000-0001-9583-3336 Vidal Sánchez, Verónica Puente Bedia, Alba Campa Fernández, Víctor Manuel Lantigua Romero, Sara Narcís Majos, Josep Oriol Velasco, Ana Bartesaghi, Renata Martínez-Cué, Carmen|||0000-0002-2137-1156 |
| author_role | author |
| collection | UCrea Repositorio Abierto de la Universidad de Cantabria |
| description | Background: The cognitive impairments that characterize Down syndrome (DS) have been attributed to brain hypocellularity due to neurogenesis impairment during fetal stages. Thus, enhancing prenatal neurogenesis in DS could prevent or reduce some of the neuromorphological and cognitive defects found in postnatal stages. Objectives: As fatty acids play a fundamental role in morphogenesis and brain development during fetal stages, in this study, we aimed to enhance neurogenesis and the cognitive abilities of the Ts65Dn (TS) mouse model of DS by administering oleic or linolenic acid. Methods: In total, 85 pregnant TS females were subcutaneously treated from Embryonic Day (ED) 10 until Postnatal Day (PD) 2 with oleic acid (400 mg/kg), linolenic acid (500 mg/kg), or vehicle. All analyses were performed on their TS and Control (CO) male and female progeny. At PD2, we evaluated the short-term effects of the treatments on neurogenesis, cellularity, and brain weight, in 40 TS and CO pups. A total of 69 TS and CO mice were used to test the long-term effects of the prenatal treatments on cognition from PD30 to PD45, and on neurogenesis, cellularity, and synaptic markers, at PD45. Data were compared by ANOVAs. Results: Prenatal administration of oleic or linolenic acid increased the brain weight (+36.7% and +45%, P < 0.01), the density of BrdU (bromodeoxyuridine)- (+80% and +115%; P < 0.01), and DAPI (4',6-diamidino-2-phenylindole)-positive cells (+64% and +22%, P < 0.05) of PD2 TS mice with respect to the vehicle-treated TS mice. Between PD30 and PD45, TS mice prenatally treated with oleic or linolenic acid showed better cognitive abilities (+28% and +25%, P < 0.01) and a higher density of the postsynaptic marker PSD95 (postsynaptic density protein 95) (+65% and +44%, P < 0.05) than the vehicle-treated TS animals. Conclusion: The beneficial cognitive and neuromorphological effects induced by oleic or linolenic acid in TS mice suggest that they could be promising pharmacotherapies for DS-associated cognitive deficits. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_49120dd2934b2f6de4d6463b3a23a935 |
| institution | Universidad de Cantabria (UC) |
| instname_str | Universidad de Cantabria (UC) |
| language | eng |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:repositorio.unican.es:10902/21334 |
| publishDate | 2020 |
| publishDateSort | 2020 |
| publisher | Oxford University Press |
| publisherStr | Oxford University Press |
| reponame_str | UCrea Repositorio Abierto de la Universidad de Cantabria |
| spelling | Prenatal Administration of Oleic Acid or Linolenic Acid Reduces Neuromorphological and Cognitive Alterations in Ts65dn Down Syndrome Mice García Cerro, Susana Rueda Revilla, Noemí|||0000-0001-9583-3336 Vidal Sánchez, Verónica Puente Bedia, Alba Campa Fernández, Víctor Manuel Lantigua Romero, Sara Narcís Majos, Josep Oriol Velasco, Ana Bartesaghi, Renata Martínez-Cué, Carmen|||0000-0002-2137-1156 Down Syndrome Ts65Dn Mice Cognition Linolenic Acid Neurogenesis Oleic Acid Prenatal Treatment Background: The cognitive impairments that characterize Down syndrome (DS) have been attributed to brain hypocellularity due to neurogenesis impairment during fetal stages. Thus, enhancing prenatal neurogenesis in DS could prevent or reduce some of the neuromorphological and cognitive defects found in postnatal stages. Objectives: As fatty acids play a fundamental role in morphogenesis and brain development during fetal stages, in this study, we aimed to enhance neurogenesis and the cognitive abilities of the Ts65Dn (TS) mouse model of DS by administering oleic or linolenic acid. Methods: In total, 85 pregnant TS females were subcutaneously treated from Embryonic Day (ED) 10 until Postnatal Day (PD) 2 with oleic acid (400 mg/kg), linolenic acid (500 mg/kg), or vehicle. All analyses were performed on their TS and Control (CO) male and female progeny. At PD2, we evaluated the short-term effects of the treatments on neurogenesis, cellularity, and brain weight, in 40 TS and CO pups. A total of 69 TS and CO mice were used to test the long-term effects of the prenatal treatments on cognition from PD30 to PD45, and on neurogenesis, cellularity, and synaptic markers, at PD45. Data were compared by ANOVAs. Results: Prenatal administration of oleic or linolenic acid increased the brain weight (+36.7% and +45%, P < 0.01), the density of BrdU (bromodeoxyuridine)- (+80% and +115%; P < 0.01), and DAPI (4',6-diamidino-2-phenylindole)-positive cells (+64% and +22%, P < 0.05) of PD2 TS mice with respect to the vehicle-treated TS mice. Between PD30 and PD45, TS mice prenatally treated with oleic or linolenic acid showed better cognitive abilities (+28% and +25%, P < 0.01) and a higher density of the postsynaptic marker PSD95 (postsynaptic density protein 95) (+65% and +44%, P < 0.05) than the vehicle-treated TS animals. Conclusion: The beneficial cognitive and neuromorphological effects induced by oleic or linolenic acid in TS mice suggest that they could be promising pharmacotherapies for DS-associated cognitive deficits. This study was supported by “Fondazione Generali e Assicurazioni Generali”, Italy; Fundación Tatiana Pérez de Guzmán el Bueno, IDIVAL (NVAL 19/23), and the Spanish Ministry of Economy and Competitiveness (PSI-2016- 76194-R, AEI/FEDER, EU). Oxford University Press http://hdl.handle.net/10902/21334 |
| spellingShingle | Prenatal Administration of Oleic Acid or Linolenic Acid Reduces Neuromorphological and Cognitive Alterations in Ts65dn Down Syndrome Mice García Cerro, Susana Down Syndrome Ts65Dn Mice Cognition Linolenic Acid Neurogenesis Oleic Acid Prenatal Treatment |
| title | Prenatal Administration of Oleic Acid or Linolenic Acid Reduces Neuromorphological and Cognitive Alterations in Ts65dn Down Syndrome Mice |
| title_full | Prenatal Administration of Oleic Acid or Linolenic Acid Reduces Neuromorphological and Cognitive Alterations in Ts65dn Down Syndrome Mice |
| title_fullStr | Prenatal Administration of Oleic Acid or Linolenic Acid Reduces Neuromorphological and Cognitive Alterations in Ts65dn Down Syndrome Mice |
| title_full_unstemmed | Prenatal Administration of Oleic Acid or Linolenic Acid Reduces Neuromorphological and Cognitive Alterations in Ts65dn Down Syndrome Mice |
| title_short | Prenatal Administration of Oleic Acid or Linolenic Acid Reduces Neuromorphological and Cognitive Alterations in Ts65dn Down Syndrome Mice |
| title_sort | Prenatal Administration of Oleic Acid or Linolenic Acid Reduces Neuromorphological and Cognitive Alterations in Ts65dn Down Syndrome Mice |
| topic | Down Syndrome Ts65Dn Mice Cognition Linolenic Acid Neurogenesis Oleic Acid Prenatal Treatment |
| topic_facet | Down Syndrome Ts65Dn Mice Cognition Linolenic Acid Neurogenesis Oleic Acid Prenatal Treatment |
| url | http://hdl.handle.net/10902/21334 |