Multichannel fluorescence microscopy: advantages of going beyond a single emission
Fluorescent microscopy has enabled the study of intracellular processes andrevealed the most intricate details of the subcellular structure. This has benefittednot only the basic biological science, but also has had an impact in numerousbiomedical applications. Basic fluorescent sensing techniques use...
| Authors: | ; ; |
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| Format: | Article |
| Publication date: | 2022 |
| Country: | España |
| Institution: | Universidad Autónoma de Madrid |
| Repository: | Biblos-e Archivo. Repositorio Institucional de la UAM |
| Language: | English |
| OAI Identifier: | oai:repositorio.uam.es:10486/714139 |
| Online Access: | http://hdl.handle.net/10486/714139 |
| Access Level: | Open access |
| Keyword: | Biomedical applications Fluorescence microscopy Intracellular sensing Multichannel Multiplexing Ratiometric sensing Física |
| Summary: | Fluorescent microscopy has enabled the study of intracellular processes andrevealed the most intricate details of the subcellular structure. This has benefittednot only the basic biological science, but also has had an impact in numerousbiomedical applications. Basic fluorescent sensing techniques use the change inthe absolute emission of a fluorescent sensor. This entails some disadvantagesas the signal might be influenced by factors not directly related to the processunder study (e.g., fluctuations in the excitation source). To overcome thesedrawbacks, one can use multiple emissions of a single or various fluorophores.There are numerous examples of multichannel fluorescence microscopy tech-niques that have given rise to numerous ratiometric methods and multiplexingassays. Herein, how the use of multiple emission channels has impacted fluo-rescence microscopy in terms of speed, sensitivity, and resolution is reviewed.Using recent examples, how the easy implementation of multichannel detectioncan overcome current limitations of the main used fluorescence techniques andpromote the development of novel microscopy methods is shown |
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