A polymorphism in the fatty acid desaturase-2 gene is associated with the arachidonic acid metabolism in pigs
Arachidonic acid (C20:4) is related to a wide range of biological effects including lipid homeostasis. The fatty acid desaturase-2 (FADS2) gene encodes for the delta-6-desaturase, which is involved in the biosynthesis of C20:4 from linoleic acid (C18:2). The purpose of this study was to characterise...
| Authors: | ; ; ; ; |
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| Format: | Article |
| Status: | Published version |
| Publication date: | 2018 |
| Country: | España |
| Institution: | Universitat de Lleida (UdL) |
| Repository: | Repositori Obert UdL |
| OAI Identifier: | oai:repositori.udl.cat:10459.1/65209 |
| Online Access: | https://doi.org/10.1038/s41598-018-32710-w http://hdl.handle.net/10459.1/65209 |
| Access Level: | Open access |
| Keyword: | Àcids grassos Porcs Animals--Cria i desenvolupament Porcs--Genètica |
| _version_ | 1878732171683823616 |
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| author | Gol, Sofia |
| author2 | Pena i Subirà, Ramona Natacha Rothschild, Max Frederick Tor i Naudí, Marc Estany Illa, Joan |
| author2_role | author author author author |
| author_browse | Estany Illa, Joan Gol, Sofia Pena i Subirà, Ramona Natacha Rothschild, Max Frederick Tor i Naudí, Marc |
| author_facet | Gol, Sofia Pena i Subirà, Ramona Natacha Rothschild, Max Frederick Tor i Naudí, Marc Estany Illa, Joan |
| author_role | author |
| collection | Repositori Obert UdL |
| description | Arachidonic acid (C20:4) is related to a wide range of biological effects including lipid homeostasis. The fatty acid desaturase-2 (FADS2) gene encodes for the delta-6-desaturase, which is involved in the biosynthesis of C20:4 from linoleic acid (C18:2). The purpose of this study was to characterise mutations in the promoter of the porcine FADS2, evaluating in particular the effect of one haplotype tagging polymorphism (rs321384923A > G) on the biosynthesis pathway of C20:4. A total of 1,192 Duroc barrows with records on fatty acid composition in muscle and subcutaneous fat were genotyped. Pigs carrying the A allele showed, irrespective of fat content, both enhanced FADS2 expression and higher C20:4 in muscle and exhibited increased ratios of C20:4 to C18:2 and of C20:4 to eicosadienoic acid (C20:2) in both muscle and adipose tissue. Despite the inverse relationship observed between C20:4 and fat content, the rs321384923 polymorphism had no impact on lean weight. It is concluded that the haplotype encompassing the rs321384923 polymorphism at the porcine FADS2 affects the n-6 fatty acid profile by specifically modifying the desaturation efficiency of C18:2 to C20:4 rather than by concomitant variations in C18:2 following changes in fat content. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_3dee33dfbc54a13bf70bd4d4e10ba663 |
| institution | Universitat de Lleida (UdL) |
| instname_str | Universitat de Lleida (UdL) |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:repositori.udl.cat:10459.1/65209 |
| publishDate | 2018 |
| publishDateSort | 2018 |
| publisher | Nature Publishing Group |
| publisherStr | Nature Publishing Group |
| reponame_str | Repositori Obert UdL |
| spelling | A polymorphism in the fatty acid desaturase-2 gene is associated with the arachidonic acid metabolism in pigs Gol, Sofia Pena i Subirà, Ramona Natacha Rothschild, Max Frederick Tor i Naudí, Marc Estany Illa, Joan Àcids grassos Porcs Animals--Cria i desenvolupament Porcs--Genètica Arachidonic acid (C20:4) is related to a wide range of biological effects including lipid homeostasis. The fatty acid desaturase-2 (FADS2) gene encodes for the delta-6-desaturase, which is involved in the biosynthesis of C20:4 from linoleic acid (C18:2). The purpose of this study was to characterise mutations in the promoter of the porcine FADS2, evaluating in particular the effect of one haplotype tagging polymorphism (rs321384923A > G) on the biosynthesis pathway of C20:4. A total of 1,192 Duroc barrows with records on fatty acid composition in muscle and subcutaneous fat were genotyped. Pigs carrying the A allele showed, irrespective of fat content, both enhanced FADS2 expression and higher C20:4 in muscle and exhibited increased ratios of C20:4 to C18:2 and of C20:4 to eicosadienoic acid (C20:2) in both muscle and adipose tissue. Despite the inverse relationship observed between C20:4 and fat content, the rs321384923 polymorphism had no impact on lean weight. It is concluded that the haplotype encompassing the rs321384923 polymorphism at the porcine FADS2 affects the n-6 fatty acid profile by specifically modifying the desaturation efficiency of C18:2 to C20:4 rather than by concomitant variations in C18:2 following changes in fat content. This research has received funding from the Spanish Ministry of Economy and Competitiveness and the European Union Regional Development Funds (AGL2015–65846-R grant) and was partially supported by the Centre for the Development of Industrial Technology (IDI-20150115 project). SG is recipient of a PhD scholarship from the Spanish Ministry of Science and Innovation (BES-2014-FPU13/04975). We acknowledge the staff at Selección Batallé for their cooperation. Nature Publishing Group https://doi.org/10.1038/s41598-018-32710-w http://hdl.handle.net/10459.1/65209 |
| spellingShingle | A polymorphism in the fatty acid desaturase-2 gene is associated with the arachidonic acid metabolism in pigs Gol, Sofia Àcids grassos Porcs Animals--Cria i desenvolupament Porcs--Genètica |
| status_str | publishedVersion |
| title | A polymorphism in the fatty acid desaturase-2 gene is associated with the arachidonic acid metabolism in pigs |
| title_full | A polymorphism in the fatty acid desaturase-2 gene is associated with the arachidonic acid metabolism in pigs |
| title_fullStr | A polymorphism in the fatty acid desaturase-2 gene is associated with the arachidonic acid metabolism in pigs |
| title_full_unstemmed | A polymorphism in the fatty acid desaturase-2 gene is associated with the arachidonic acid metabolism in pigs |
| title_short | A polymorphism in the fatty acid desaturase-2 gene is associated with the arachidonic acid metabolism in pigs |
| title_sort | A polymorphism in the fatty acid desaturase-2 gene is associated with the arachidonic acid metabolism in pigs |
| topic | Àcids grassos Porcs Animals--Cria i desenvolupament Porcs--Genètica |
| topic_facet | Àcids grassos Porcs Animals--Cria i desenvolupament Porcs--Genètica |
| url | https://doi.org/10.1038/s41598-018-32710-w http://hdl.handle.net/10459.1/65209 |