Selection of aptamers against prostate specific antigen for diagnostic and therapeutic applications
Currently, PSA is considered the most sensitive marker available for prostate cancer detection and for monitoring its progression. To date monoclonal/polyclonal antibodies have been used to detect PSA due to their high specificity and sensitivity. However, the production of antibodies is time-consum...
| Autor: | |
|---|---|
| Tipo de documento: | Tesis doctoral |
| Estado: | Published version |
| Fecha de publicación: | 2012 |
| País: | España |
| Institución: | Universitat Rovira i virgili (URV) |
| Repositorio: | Repositori Institucional de la Universitat Rovira i Virgili |
| OAI Identifier: | oai:urv.cat:TDX:1072 |
| Acceso en línea: | https://hdl.handle.net/20.500.11797/TDX1072 http://hdl.handle.net/10803/83492 |
| Nivel de acceso: | Acceso abierto |
| Palabra clave: | 577 - Bioquímica. Biologia molecular. Biofísica |
| _version_ | 1878731839137382400 |
|---|---|
| author | Svobodová, Markéta |
| author_browse | Svobodová, Markéta |
| author_facet | Svobodová, Markéta |
| author_role | author |
| collection | Repositori Institucional de la Universitat Rovira i Virgili |
| description | Currently, PSA is considered the most sensitive marker available for prostate cancer detection and for monitoring its progression. To date monoclonal/polyclonal antibodies have been used to detect PSA due to their high specificity and sensitivity. However, the production of antibodies is time-consuming and expensive. On the other hand aptamers with specificity and affinity equal to those of antibodies could be an alternative way for the detection of PSA. This work overviews the selection of aptamers against prostate specific antigen (PSA). Fundamental aspects such as the characterization of PSA, evaluation of immobilization strategies, the preparation of oligonucleotide library and single stranded DNA (ssDNA) have been evaluated in order to reach the main objective of this thesis. Finally, selection of DNA and RNA based aptamers against PSA and their potential use in diagnostic and therapeutic applications have been described |
| eu_rights_str_mv | openAccess |
| format | doctoralThesis |
| id | ES_37a39ee6dcfd1c1ff7f94d19353f20f0 |
| institution | Universitat Rovira i virgili (URV) |
| instname_str | Universitat Rovira i virgili (URV) |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:urv.cat:TDX:1072 |
| publishDate | 2012 |
| publishDateSort | 2012 |
| publisher | Universitat Rovira i Virgili |
| publisherStr | Universitat Rovira i Virgili |
| reponame_str | Repositori Institucional de la Universitat Rovira i Virgili |
| spelling | Selection of aptamers against prostate specific antigen for diagnostic and therapeutic applications Svobodová, Markéta 577 - Bioquímica. Biologia molecular. Biofísica Currently, PSA is considered the most sensitive marker available for prostate cancer detection and for monitoring its progression. To date monoclonal/polyclonal antibodies have been used to detect PSA due to their high specificity and sensitivity. However, the production of antibodies is time-consuming and expensive. On the other hand aptamers with specificity and affinity equal to those of antibodies could be an alternative way for the detection of PSA. This work overviews the selection of aptamers against prostate specific antigen (PSA). Fundamental aspects such as the characterization of PSA, evaluation of immobilization strategies, the preparation of oligonucleotide library and single stranded DNA (ssDNA) have been evaluated in order to reach the main objective of this thesis. Finally, selection of DNA and RNA based aptamers against PSA and their potential use in diagnostic and therapeutic applications have been described Actualmente, el antígeno prostático específico (PSA) se considera el marcador más sensible para la detección de cáncer de próstata. Hasta la fecha anticuerpos monoclonales y policlonales se han utilizado para detectar el PSA debido a su alta especificidad y sensibilidad. Sin embargo, la producción de anticuerpos es lenta y cara. En el otro lado, los aptámeros con especificidad y afinidad iguales a los de los anticuerpos podría ser una forma alternativa para la detección de PSA. El objetivo de este trabajo es la selección de aptámeros contra el PSA. Aspectos fundamentales como la caracterización de la PSA, la evaluación de las estrategias de inmovilización, la preparación de la biblioteca de oligonucleótidos y de la cadena simple de ADN (ssDNA) han sido evaluados con el fin de alcanzar el objetivo principal de esta tesis. Finalmente, la selección de aptámeros contra el PSA y su uso potencial en aplicaciones de diagnóstica y terapéutica han sido descritos. Universitat Rovira i Virgili https://hdl.handle.net/20.500.11797/TDX1072 http://hdl.handle.net/10803/83492 |
| spellingShingle | Selection of aptamers against prostate specific antigen for diagnostic and therapeutic applications Svobodová, Markéta 577 - Bioquímica. Biologia molecular. Biofísica |
| status_str | publishedVersion |
| title | Selection of aptamers against prostate specific antigen for diagnostic and therapeutic applications |
| title_full | Selection of aptamers against prostate specific antigen for diagnostic and therapeutic applications |
| title_fullStr | Selection of aptamers against prostate specific antigen for diagnostic and therapeutic applications |
| title_full_unstemmed | Selection of aptamers against prostate specific antigen for diagnostic and therapeutic applications |
| title_short | Selection of aptamers against prostate specific antigen for diagnostic and therapeutic applications |
| title_sort | Selection of aptamers against prostate specific antigen for diagnostic and therapeutic applications |
| topic | 577 - Bioquímica. Biologia molecular. Biofísica |
| topic_facet | 577 - Bioquímica. Biologia molecular. Biofísica |
| url | https://hdl.handle.net/20.500.11797/TDX1072 http://hdl.handle.net/10803/83492 |