The PSMA8 subunit of the spermatoproteasome is essential for proper meiotic exit and mouse fertility
[EN]The ubiquitin proteasome system regulates meiotic recombination in yeast through its association with the synaptonemal complex, a ‘zipper’-like structure that holds homologous chromosome pairs in synapsis during meiotic prophase I. In mammals, the proteasome activator subunit PA200 targets acety...
| Authors: | ; ; ; ; ; ; ; ; ; ; ; |
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| Format: | Article |
| Publication date: | 2019 |
| Country: | España |
| Institution: | Universidad de Salamanca (USAL) |
| Repository: | GREDOS. Repositorio Institucional de la Universidad de Salamanca |
| OAI Identifier: | oai:gredos.usal.es:10366/143907 |
| Online Access: | http://hdl.handle.net/10366/143907 |
| Access Level: | Open access |
| Keyword: | Meiotic prophase Spermatogenesis Proteasomes Protein extraction Meiotic Prophase I 2415 Biología Molecular profase meiótica I espermatogénesis |
| _version_ | 1878731660163284992 |
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| author | Gómez Hernández, Laura |
| author2 | Felipe-Medina, Natalia Condezo, Yazmine B. García-Valiente, Rodrigo Ramos, Isabel Suja, José Angel Barbero, José Luis Roig, Ignasi Sánchez Martín, Manuel Adolfo de Rooij, Dirk G. Llano Cuadra, María Elena Martín Pendás, Alberto |
| author2_role | author author author author author author author author author author author |
| author_browse | Barbero, José Luis Condezo, Yazmine B. Felipe-Medina, Natalia García-Valiente, Rodrigo Gómez Hernández, Laura Llano Cuadra, María Elena Martín Pendás, Alberto Ramos, Isabel Roig, Ignasi Suja, José Angel Sánchez Martín, Manuel Adolfo de Rooij, Dirk G. |
| author_facet | Gómez Hernández, Laura Felipe-Medina, Natalia Condezo, Yazmine B. García-Valiente, Rodrigo Ramos, Isabel Suja, José Angel Barbero, José Luis Roig, Ignasi Sánchez Martín, Manuel Adolfo de Rooij, Dirk G. Llano Cuadra, María Elena Martín Pendás, Alberto |
| author_role | author |
| collection | GREDOS. Repositorio Institucional de la Universidad de Salamanca |
| description | [EN]The ubiquitin proteasome system regulates meiotic recombination in yeast through its association with the synaptonemal complex, a ‘zipper’-like structure that holds homologous chromosome pairs in synapsis during meiotic prophase I. In mammals, the proteasome activator subunit PA200 targets acetylated histones for degradation during somatic DNA double strand break repair and during histone replacement during spermiogenesis. We investigated the role of the testis-specific proteasomal subunit α4s (PSMA8) during spermatogenesis, and found that PSMA8 was localized to and dependent on the central region of the synaptonemal complex. Accordingly, synapsis-deficient mice show delocalization of PSMA8. Moreover, though Psma8-deficient mice are proficient in meiotic homologous recombination, there are alterations in the proteostasis of several key meiotic players that, in addition to the known substrate acetylated histones, have been shown by a proteomic approach to interact with PSMA8, such as SYCP3, SYCP1, CDK1 and TRIP13. These alterations lead to an accumulation of spermatocytes in metaphase I and II which either enter massively into apoptosis or give rise to a low number of aberrant round spermatids that apoptose before histone replacement takes place. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_33ff2e4e8a47dfbbbf3755c99b332004 |
| institution | Universidad de Salamanca (USAL) |
| instname_str | Universidad de Salamanca (USAL) |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:gredos.usal.es:10366/143907 |
| publishDate | 2019 |
| publishDateSort | 2019 |
| publisher | Public Library of Science (New York) |
| publisherStr | Public Library of Science (New York) |
| reponame_str | GREDOS. Repositorio Institucional de la Universidad de Salamanca |
| spelling | The PSMA8 subunit of the spermatoproteasome is essential for proper meiotic exit and mouse fertility Gómez Hernández, Laura Felipe-Medina, Natalia Condezo, Yazmine B. García-Valiente, Rodrigo Ramos, Isabel Suja, José Angel Barbero, José Luis Roig, Ignasi Sánchez Martín, Manuel Adolfo de Rooij, Dirk G. Llano Cuadra, María Elena Martín Pendás, Alberto Meiotic prophase Spermatogenesis Proteasomes Protein extraction Meiotic Prophase I 2415 Biología Molecular profase meiótica I espermatogénesis [EN]The ubiquitin proteasome system regulates meiotic recombination in yeast through its association with the synaptonemal complex, a ‘zipper’-like structure that holds homologous chromosome pairs in synapsis during meiotic prophase I. In mammals, the proteasome activator subunit PA200 targets acetylated histones for degradation during somatic DNA double strand break repair and during histone replacement during spermiogenesis. We investigated the role of the testis-specific proteasomal subunit α4s (PSMA8) during spermatogenesis, and found that PSMA8 was localized to and dependent on the central region of the synaptonemal complex. Accordingly, synapsis-deficient mice show delocalization of PSMA8. Moreover, though Psma8-deficient mice are proficient in meiotic homologous recombination, there are alterations in the proteostasis of several key meiotic players that, in addition to the known substrate acetylated histones, have been shown by a proteomic approach to interact with PSMA8, such as SYCP3, SYCP1, CDK1 and TRIP13. These alterations lead to an accumulation of spermatocytes in metaphase I and II which either enter massively into apoptosis or give rise to a low number of aberrant round spermatids that apoptose before histone replacement takes place. MINECO ; Junta de Castilla y León Public Library of Science (New York) http://hdl.handle.net/10366/143907 |
| spellingShingle | The PSMA8 subunit of the spermatoproteasome is essential for proper meiotic exit and mouse fertility Gómez Hernández, Laura Meiotic prophase Spermatogenesis Proteasomes Protein extraction Meiotic Prophase I 2415 Biología Molecular profase meiótica I espermatogénesis |
| title | The PSMA8 subunit of the spermatoproteasome is essential for proper meiotic exit and mouse fertility |
| title_full | The PSMA8 subunit of the spermatoproteasome is essential for proper meiotic exit and mouse fertility |
| title_fullStr | The PSMA8 subunit of the spermatoproteasome is essential for proper meiotic exit and mouse fertility |
| title_full_unstemmed | The PSMA8 subunit of the spermatoproteasome is essential for proper meiotic exit and mouse fertility |
| title_short | The PSMA8 subunit of the spermatoproteasome is essential for proper meiotic exit and mouse fertility |
| title_sort | The PSMA8 subunit of the spermatoproteasome is essential for proper meiotic exit and mouse fertility |
| topic | Meiotic prophase Spermatogenesis Proteasomes Protein extraction Meiotic Prophase I 2415 Biología Molecular profase meiótica I espermatogénesis |
| topic_facet | Meiotic prophase Spermatogenesis Proteasomes Protein extraction Meiotic Prophase I 2415 Biología Molecular profase meiótica I espermatogénesis |
| url | http://hdl.handle.net/10366/143907 |