Analagous population structures for two alphabaculoviruses highlight a functional role for deletion mutants
A natural Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV) isolate from Florida shares a strikingly similar genotypic composition to that of a natural Spodoptera frugiperda MNPV (SfMNPV) isolate from Nicaragua. Both isolates comprise a high proportion of large-deletion genotypes that lack ge...
| Authors: | ; ; ; ; ; |
|---|---|
| Format: | Article |
| Status: | Published version |
| Publication date: | 2012 |
| Country: | España |
| Institution: | Universidad Pública de Navarra |
| Repository: | Academica-e. Repositorio Institucional de la Universidad Pública de Navarra |
| OAI Identifier: | oai:academica-e.unavarra.es:2454/31944 |
| Online Access: | https://hdl.handle.net/2454/31944 |
| Access Level: | Open access |
| Keyword: | Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV) Spodoptera frugiperda MNPV (SfMNPV) Occlusion bodies (OBs) |
| _version_ | 1878731403037769728 |
|---|---|
| author | Serrano García, Amaya |
| author2 | Williams, Trevor Simón de Goñi, Oihane López Ferber, Miguel Caballero Murillo, Primitivo Muñoz Labiano, Delia |
| author2_role | author author author author author |
| author_browse | Caballero Murillo, Primitivo López Ferber, Miguel Muñoz Labiano, Delia Serrano García, Amaya Simón de Goñi, Oihane Williams, Trevor |
| author_facet | Serrano García, Amaya Williams, Trevor Simón de Goñi, Oihane López Ferber, Miguel Caballero Murillo, Primitivo Muñoz Labiano, Delia |
| author_role | author |
| collection | Academica-e. Repositorio Institucional de la Universidad Pública de Navarra |
| description | A natural Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV) isolate from Florida shares a strikingly similar genotypic composition to that of a natural Spodoptera frugiperda MNPV (SfMNPV) isolate from Nicaragua. Both isolates comprise a high proportion of large-deletion genotypes that lack genes that are essential for viral replication or transmission. To determine the likely origins of such genotypically similar population structures, we performed genomic and functional analyses of these genotypes. The homology of nucleotides in the deleted regions was as high as 79%, similar to those of other colinear genomic regions, although some SfMNPV genes were not present in SeMNPV. In addition, no potential consensus sequences were shared between the deletion flanking sequences. These results indicate an evolutionary mechanism that independently generates and sustains deletion mutants within each virus population. Functional analyses using different proportions of complete and deletion genotypes were performed with the two viruses in mixtures of occlusion bodies (OBs) or co-occluded virions. Ratios greater than 3:1 of complete/deletion genotypes resulted in reduced pathogenicity (expressed as median lethal dose), but there were no significant changes in the speed of kill. In contrast, OB yields increased only in the 1:1 mixture. The three phenotypic traits analyzed provide a broader picture of the functional significance of the most extensively deleted SeMNPV genotype and contribute toward the elucidation of the role of such mutants in baculovirus populations. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_2ee788e79d4b21e4e02e6b4e8cd47508 |
| institution | Universidad Pública de Navarra |
| instname_str | Universidad Pública de Navarra |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:academica-e.unavarra.es:2454/31944 |
| publishDate | 2012 |
| publishDateSort | 2012 |
| publisher | American Society for Microbiology |
| publisherStr | American Society for Microbiology |
| reponame_str | Academica-e. Repositorio Institucional de la Universidad Pública de Navarra |
| spelling | Analagous population structures for two alphabaculoviruses highlight a functional role for deletion mutants Serrano García, Amaya Williams, Trevor Simón de Goñi, Oihane López Ferber, Miguel Caballero Murillo, Primitivo Muñoz Labiano, Delia Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV) Spodoptera frugiperda MNPV (SfMNPV) Occlusion bodies (OBs) A natural Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV) isolate from Florida shares a strikingly similar genotypic composition to that of a natural Spodoptera frugiperda MNPV (SfMNPV) isolate from Nicaragua. Both isolates comprise a high proportion of large-deletion genotypes that lack genes that are essential for viral replication or transmission. To determine the likely origins of such genotypically similar population structures, we performed genomic and functional analyses of these genotypes. The homology of nucleotides in the deleted regions was as high as 79%, similar to those of other colinear genomic regions, although some SfMNPV genes were not present in SeMNPV. In addition, no potential consensus sequences were shared between the deletion flanking sequences. These results indicate an evolutionary mechanism that independently generates and sustains deletion mutants within each virus population. Functional analyses using different proportions of complete and deletion genotypes were performed with the two viruses in mixtures of occlusion bodies (OBs) or co-occluded virions. Ratios greater than 3:1 of complete/deletion genotypes resulted in reduced pathogenicity (expressed as median lethal dose), but there were no significant changes in the speed of kill. In contrast, OB yields increased only in the 1:1 mixture. The three phenotypic traits analyzed provide a broader picture of the functional significance of the most extensively deleted SeMNPV genotype and contribute toward the elucidation of the role of such mutants in baculovirus populations. This study received financial support from the Spanish Ministry for Science and Technology (AGL2008-05456-C03-01). A.S. received a predoctoral fellowship from the Spanish Ministry of Education and Culture. American Society for Microbiology https://hdl.handle.net/2454/31944 |
| spellingShingle | Analagous population structures for two alphabaculoviruses highlight a functional role for deletion mutants Serrano García, Amaya Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV) Spodoptera frugiperda MNPV (SfMNPV) Occlusion bodies (OBs) |
| status_str | publishedVersion |
| title | Analagous population structures for two alphabaculoviruses highlight a functional role for deletion mutants |
| title_full | Analagous population structures for two alphabaculoviruses highlight a functional role for deletion mutants |
| title_fullStr | Analagous population structures for two alphabaculoviruses highlight a functional role for deletion mutants |
| title_full_unstemmed | Analagous population structures for two alphabaculoviruses highlight a functional role for deletion mutants |
| title_short | Analagous population structures for two alphabaculoviruses highlight a functional role for deletion mutants |
| title_sort | Analagous population structures for two alphabaculoviruses highlight a functional role for deletion mutants |
| topic | Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV) Spodoptera frugiperda MNPV (SfMNPV) Occlusion bodies (OBs) |
| topic_facet | Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV) Spodoptera frugiperda MNPV (SfMNPV) Occlusion bodies (OBs) |
| url | https://hdl.handle.net/2454/31944 |