Classical Swine Fever Virus vs. Classical Swine Fever Virus
Two groups with three wild boars each were used: Group A (animals 1 to 3) served as the control, and Group B (animals 4 to 6) was postnatally persistently infected with the Cat01 strain of CSFV (primary virus). The animals, six weeks old and clinically healthy, were inoculated with the virulent stra...
| Authors: | ; ; ; ; ; ; ; ; ; ; |
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| Format: | Article |
| Publication date: | 2016 |
| Country: | España |
| Institution: | Universitat Autònoma de Barcelona |
| Repository: | Dipòsit Digital de Documents de la UAB |
| Language: | English |
| OAI Identifier: | oai:ddd.uab.cat:170615 |
| Online Access: | https://ddd.uab.cat/record/170615 https://dx.doi.org/urn:doi:10.1371/journal.pone.0149469 |
| Access Level: | Open access |
| Keyword: | RNA extraction Swine Superinfection Enzyme-linked immunoassays Sequence analysis Viral persistence and latency Immune response RNA viruses |
| _version_ | 1878730864808951808 |
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| author | Muñoz González, Sara |
| author2 | Pérez-Simó, Marta Colom Cadena, Andreu|||0000-0003-0983-2412 Cabezón Ponsoda, Óscar|||0000-0001-7543-8371 Bohórquez Garzón, José Alejandro|||0000-0002-6715-1757 Rosell, Rosa|||0000-0002-8294-1159 Pérez, Lester Josue|||0000-0002-5717-5181 Marco, Ignasi|||0000-0001-7479-3419 Lavín González, Santiago|||0000-0001-5655-588X Domingo, Mariano|||0000-0002-9623-4826 Ganges, Llilianne|||0000-0002-8644-3560 |
| author2_role | author author author author author author author author author author |
| author_browse | Bohórquez Garzón, José Alejandro|||0000-0002-6715-1757 Cabezón Ponsoda, Óscar|||0000-0001-7543-8371 Colom Cadena, Andreu|||0000-0003-0983-2412 Domingo, Mariano|||0000-0002-9623-4826 Ganges, Llilianne|||0000-0002-8644-3560 Lavín González, Santiago|||0000-0001-5655-588X Marco, Ignasi|||0000-0001-7479-3419 Muñoz González, Sara Pérez, Lester Josue|||0000-0002-5717-5181 Pérez-Simó, Marta Rosell, Rosa|||0000-0002-8294-1159 |
| author_facet | Muñoz González, Sara Pérez-Simó, Marta Colom Cadena, Andreu|||0000-0003-0983-2412 Cabezón Ponsoda, Óscar|||0000-0001-7543-8371 Bohórquez Garzón, José Alejandro|||0000-0002-6715-1757 Rosell, Rosa|||0000-0002-8294-1159 Pérez, Lester Josue|||0000-0002-5717-5181 Marco, Ignasi|||0000-0001-7479-3419 Lavín González, Santiago|||0000-0001-5655-588X Domingo, Mariano|||0000-0002-9623-4826 Ganges, Llilianne|||0000-0002-8644-3560 |
| author_role | author |
| collection | Dipòsit Digital de Documents de la UAB |
| description | Two groups with three wild boars each were used: Group A (animals 1 to 3) served as the control, and Group B (animals 4 to 6) was postnatally persistently infected with the Cat01 strain of CSFV (primary virus). The animals, six weeks old and clinically healthy, were inoculated with the virulent strain Margarita (secondary virus). For exclusive detection of the Margarita strain, a specific qRT-PCR assay was designed, which proved not to have cross-reactivity with the Cat01 strain. The wild boars persistently infected with CSFV were protected from superinfection by the virulent CSFV Margarita strain, as evidenced by the absence of clinical signs and the absence of Margarita RNA detection in serum, swabs and tissue samples. Additionally, in PBMCs, a well-known target for CSFV viral replication, only the primary infecting virus RNA (Cat01 strain) could be detected, even after the isolation in ST cells, demonstrating SIE at the tissue level in vivo. Furthermore, the data analysis of the Margarita qRT-PCR, by means of calculated ΔCt values, supported that PBMCs from persistently infected animals were substantially protected from superinfection after in vitro inoculation with the Margarita virus strain, while this virus was able to infect naive PBMCs efficiently. In parallel, IFN-α values were undetectable in the sera from animals in Group B after inoculation with the CSFV Margarita strain. Furthermore, these animals were unable to elicit adaptive humoral (no E2-specific or neutralising antibodies) or cellular immune responses (in terms of IFN-γ-producing cells) after inoculation with the second virus. Finally, a sequence analysis could not detect CSFV Margarita RNA in the samples tested from Group B. Our results suggested that the SIE phenomenon might be involved in the evolution and phylogeny of the virus, as well as in CSFV control by vaccination. To the best of our knowledge, this study was one of the first showing efficient suppression of superinfection in animals, especially in the absence of IFN-α, which might be associated with the lack of innate immune mechanisms. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_24cc19d35bbd60fa9bc3a8f4ef44fc4e |
| institution | Universitat Autònoma de Barcelona |
| instname_str | Universitat Autònoma de Barcelona |
| language | eng |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:ddd.uab.cat:170615 |
| publishDate | 2016 |
| publishDateSort | 2016 |
| reponame_str | Dipòsit Digital de Documents de la UAB |
| spelling | Classical Swine Fever Virus vs. Classical Swine Fever Virus the Superinfection Exclusion Phenomenon in Experimentally Infected Wild Boar Muñoz González, Sara Pérez-Simó, Marta Colom Cadena, Andreu|||0000-0003-0983-2412 Cabezón Ponsoda, Óscar|||0000-0001-7543-8371 Bohórquez Garzón, José Alejandro|||0000-0002-6715-1757 Rosell, Rosa|||0000-0002-8294-1159 Pérez, Lester Josue|||0000-0002-5717-5181 Marco, Ignasi|||0000-0001-7479-3419 Lavín González, Santiago|||0000-0001-5655-588X Domingo, Mariano|||0000-0002-9623-4826 Ganges, Llilianne|||0000-0002-8644-3560 RNA extraction Swine Superinfection Enzyme-linked immunoassays Sequence analysis Viral persistence and latency Immune response RNA viruses Two groups with three wild boars each were used: Group A (animals 1 to 3) served as the control, and Group B (animals 4 to 6) was postnatally persistently infected with the Cat01 strain of CSFV (primary virus). The animals, six weeks old and clinically healthy, were inoculated with the virulent strain Margarita (secondary virus). For exclusive detection of the Margarita strain, a specific qRT-PCR assay was designed, which proved not to have cross-reactivity with the Cat01 strain. The wild boars persistently infected with CSFV were protected from superinfection by the virulent CSFV Margarita strain, as evidenced by the absence of clinical signs and the absence of Margarita RNA detection in serum, swabs and tissue samples. Additionally, in PBMCs, a well-known target for CSFV viral replication, only the primary infecting virus RNA (Cat01 strain) could be detected, even after the isolation in ST cells, demonstrating SIE at the tissue level in vivo. Furthermore, the data analysis of the Margarita qRT-PCR, by means of calculated ΔCt values, supported that PBMCs from persistently infected animals were substantially protected from superinfection after in vitro inoculation with the Margarita virus strain, while this virus was able to infect naive PBMCs efficiently. In parallel, IFN-α values were undetectable in the sera from animals in Group B after inoculation with the CSFV Margarita strain. Furthermore, these animals were unable to elicit adaptive humoral (no E2-specific or neutralising antibodies) or cellular immune responses (in terms of IFN-γ-producing cells) after inoculation with the second virus. Finally, a sequence analysis could not detect CSFV Margarita RNA in the samples tested from Group B. Our results suggested that the SIE phenomenon might be involved in the evolution and phylogeny of the virus, as well as in CSFV control by vaccination. To the best of our knowledge, this study was one of the first showing efficient suppression of superinfection in animals, especially in the absence of IFN-α, which might be associated with the lack of innate immune mechanisms. https://ddd.uab.cat/record/170615 https://dx.doi.org/urn:doi:10.1371/journal.pone.0149469 |
| spellingShingle | Classical Swine Fever Virus vs. Classical Swine Fever Virus Muñoz González, Sara RNA extraction Swine Superinfection Enzyme-linked immunoassays Sequence analysis Viral persistence and latency Immune response RNA viruses |
| title | Classical Swine Fever Virus vs. Classical Swine Fever Virus |
| title_full | Classical Swine Fever Virus vs. Classical Swine Fever Virus |
| title_fullStr | Classical Swine Fever Virus vs. Classical Swine Fever Virus |
| title_full_unstemmed | Classical Swine Fever Virus vs. Classical Swine Fever Virus |
| title_short | Classical Swine Fever Virus vs. Classical Swine Fever Virus |
| title_sort | Classical Swine Fever Virus vs. Classical Swine Fever Virus |
| topic | RNA extraction Swine Superinfection Enzyme-linked immunoassays Sequence analysis Viral persistence and latency Immune response RNA viruses |
| topic_facet | RNA extraction Swine Superinfection Enzyme-linked immunoassays Sequence analysis Viral persistence and latency Immune response RNA viruses |
| url | https://ddd.uab.cat/record/170615 https://dx.doi.org/urn:doi:10.1371/journal.pone.0149469 |