Detection of fungal trunk pathogens from wood tissues and pruning wood debris of olive trees in Iran
[EN] Between April 2015 and August 2017 a survey was conducted in olive orchards in different regions in Iran in order to collect and identify fungal trunk pathogens. Wood samples were collected from the trunk and branches of olive trees showing dieback and cankers, as well as pruning wood debris le...
| Authors: | ; ; ; |
|---|---|
| Format: | Article |
| Publication date: | 2025 |
| Country: | España |
| Institution: | Universitat Politècnica de València (UPV) |
| Repository: | RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
| Language: | English |
| OAI Identifier: | oai:riunet.upv.es:10251/221648 |
| Online Access: | https://riunet.upv.es/handle/10251/221648 |
| Access Level: | Embargoed access |
| Keyword: | Botryosphaeriaceae Fungal pathogens Olea europaea Fungal trunk diseases Pruning debris |
| _version_ | 1878730803180994560 |
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| author | Sohrabi, Mahboobeh |
| author2 | Mohammadi, Hamid León Santana, Maela|||0000-0003-0693-6988 Armengol Fortí, Josep|||0000-0003-3815-8578 |
| author2_role | author author author |
| author_browse | Armengol Fortí, Josep|||0000-0003-3815-8578 León Santana, Maela|||0000-0003-0693-6988 Mohammadi, Hamid Sohrabi, Mahboobeh |
| author_facet | Sohrabi, Mahboobeh Mohammadi, Hamid León Santana, Maela|||0000-0003-0693-6988 Armengol Fortí, Josep|||0000-0003-3815-8578 |
| author_role | author |
| collection | RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
| description | [EN] Between April 2015 and August 2017 a survey was conducted in olive orchards in different regions in Iran in order to collect and identify fungal trunk pathogens. Wood samples were collected from the trunk and branches of olive trees showing dieback and cankers, as well as pruning wood debris left in the orchards. Fungal isolation was performed from necrotic woody tissues, as well as fruiting bodies observed on bark of wood debris. Morphological identification of the fungal isolates was confirmed by DNA sequencing and comparisons of the internal transcribed spacer (ITS) nrDNA region and a partial sequence of translation elongation factor 1-alpha (tef-1 alpha) and beta-tubulin (tub2) genes. In this study, eight species of Botryosphaeriaceae namely Botryosphaeria (B.) dothidea, Diplodia (D.) gallae, D. intermedia, D. mutila, Dothiorella (Do.) sarmentorum, Neofusicoccum (Neof.) parvum, Neoscytalidium (Neos.) dimidiatum (as the most abundant species) and Neos. novaehollandiae were identified. Moreover, Pleurostoma (Pl.) richardsiae, Paecilomyces (P.) formosus, a Coniophora-like fungus and several isolates of Phoma, Chaetomium, Darksidea and Hypoxylon species were obtained. Pathogenicity trials on detached shoots of olive trees conducted under greenhouse conditions showed that P. formosus, Neos. dimidiatum, D. gallae, Neof. parvum, Dot. sarmentorum, Neos. novaehollandiae, Pl. richardsiae, B. dothidea, D. mutila, D. intermedia and Coniophora-like fungus were pathogenic on inoculated olive shoots. Paecilomyces formosus was the most virulent and caused the longest lesions. Based on literature reviews, this study represents the first report on the occurrence of D. mutila, Neof. parvum and Pl. richardsiae from diseased olive trees in Iran. Moreover, this is the first report of D. gallae from fruiting bodies on the bark of branches, and four species (namely D. intermedia, Neos. novaehollandiae, P. formosus and Coniophora-like fungus) from affected olive trees worldwide. Our results provide new insights into the etiology of olive fungal trunk diseases. |
| eu_rights_str_mv | embargoedAccess |
| format | article |
| id | ES_23aaee72e93b68e26d4603c0a2eeb4e7 |
| institution | Universitat Politècnica de València (UPV) |
| instname_str | Universitat Politècnica de València (UPV) |
| language | eng |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:riunet.upv.es:10251/221648 |
| publishDate | 2025 |
| publishDateSort | 2025 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| reponame_str | RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
| spelling | Detection of fungal trunk pathogens from wood tissues and pruning wood debris of olive trees in Iran Sohrabi, Mahboobeh Mohammadi, Hamid León Santana, Maela|||0000-0003-0693-6988 Armengol Fortí, Josep|||0000-0003-3815-8578 Botryosphaeriaceae Fungal pathogens Olea europaea Fungal trunk diseases Pruning debris [EN] Between April 2015 and August 2017 a survey was conducted in olive orchards in different regions in Iran in order to collect and identify fungal trunk pathogens. Wood samples were collected from the trunk and branches of olive trees showing dieback and cankers, as well as pruning wood debris left in the orchards. Fungal isolation was performed from necrotic woody tissues, as well as fruiting bodies observed on bark of wood debris. Morphological identification of the fungal isolates was confirmed by DNA sequencing and comparisons of the internal transcribed spacer (ITS) nrDNA region and a partial sequence of translation elongation factor 1-alpha (tef-1 alpha) and beta-tubulin (tub2) genes. In this study, eight species of Botryosphaeriaceae namely Botryosphaeria (B.) dothidea, Diplodia (D.) gallae, D. intermedia, D. mutila, Dothiorella (Do.) sarmentorum, Neofusicoccum (Neof.) parvum, Neoscytalidium (Neos.) dimidiatum (as the most abundant species) and Neos. novaehollandiae were identified. Moreover, Pleurostoma (Pl.) richardsiae, Paecilomyces (P.) formosus, a Coniophora-like fungus and several isolates of Phoma, Chaetomium, Darksidea and Hypoxylon species were obtained. Pathogenicity trials on detached shoots of olive trees conducted under greenhouse conditions showed that P. formosus, Neos. dimidiatum, D. gallae, Neof. parvum, Dot. sarmentorum, Neos. novaehollandiae, Pl. richardsiae, B. dothidea, D. mutila, D. intermedia and Coniophora-like fungus were pathogenic on inoculated olive shoots. Paecilomyces formosus was the most virulent and caused the longest lesions. Based on literature reviews, this study represents the first report on the occurrence of D. mutila, Neof. parvum and Pl. richardsiae from diseased olive trees in Iran. Moreover, this is the first report of D. gallae from fruiting bodies on the bark of branches, and four species (namely D. intermedia, Neos. novaehollandiae, P. formosus and Coniophora-like fungus) from affected olive trees worldwide. Our results provide new insights into the etiology of olive fungal trunk diseases. Financial support by the Iranian Ministry of Science, Research and Technology (MSRT) for the first author during her 4 months stay in Spain as a part of her PhD project is greatly acknowledged. Elsevier https://riunet.upv.es/handle/10251/221648 |
| spellingShingle | Detection of fungal trunk pathogens from wood tissues and pruning wood debris of olive trees in Iran Sohrabi, Mahboobeh Botryosphaeriaceae Fungal pathogens Olea europaea Fungal trunk diseases Pruning debris |
| title | Detection of fungal trunk pathogens from wood tissues and pruning wood debris of olive trees in Iran |
| title_full | Detection of fungal trunk pathogens from wood tissues and pruning wood debris of olive trees in Iran |
| title_fullStr | Detection of fungal trunk pathogens from wood tissues and pruning wood debris of olive trees in Iran |
| title_full_unstemmed | Detection of fungal trunk pathogens from wood tissues and pruning wood debris of olive trees in Iran |
| title_short | Detection of fungal trunk pathogens from wood tissues and pruning wood debris of olive trees in Iran |
| title_sort | Detection of fungal trunk pathogens from wood tissues and pruning wood debris of olive trees in Iran |
| topic | Botryosphaeriaceae Fungal pathogens Olea europaea Fungal trunk diseases Pruning debris |
| topic_facet | Botryosphaeriaceae Fungal pathogens Olea europaea Fungal trunk diseases Pruning debris |
| url | https://riunet.upv.es/handle/10251/221648 |