New Method for Simultaneous Determination of Microcystins and Cylindrospermopsin in Vegetable Matrices by SPE-UPLC-MS/MS
Cyanotoxins are a large group of noxious metabolites with different chemical structure and mechanisms of action, with a worldwide distribution, producing effects in animals, humans, and crop plants. When cyanotoxin-contaminated waters are used for the irrigation of edible vegetables, humans can be i...
| Autores: | ; ; ; ; ; ; ; |
|---|---|
| Tipo de documento: | Artículo |
| Fecha de publicación: | 2018 |
| País: | España |
| Institución: | Universidad Pablo de Olavide (UPO) |
| Repositorio: | RIO. Repositorio Institucional Olavide |
| Lenguaje: | Inglés |
| OAI Identifier: | oai:rio.upo.es:10433/19837 |
| Acceso en línea: | https://hdl.handle.net/10433/19837 |
| Nivel de acceso: | Acceso abierto |
| Palabra clave: | Microcystins Cylindrospermopsin UPLC-MS/MS |
| _version_ | 1878730678514745344 |
|---|---|
| author | Diez-Quijada, Leticia |
| author2 | Guzmán-Guillén, Remedios Prieto, Ana Isabel Llana Ruíz-Cabello, María Campos, Alexandre Vasconcelos, Vitor Jos, Angeles Cameán, Ana María |
| author2_role | author author author author author author author |
| author_browse | Cameán, Ana María Campos, Alexandre Diez-Quijada, Leticia Guzmán-Guillén, Remedios Jos, Angeles Llana Ruíz-Cabello, María Prieto, Ana Isabel Vasconcelos, Vitor |
| author_facet | Diez-Quijada, Leticia Guzmán-Guillén, Remedios Prieto, Ana Isabel Llana Ruíz-Cabello, María Campos, Alexandre Vasconcelos, Vitor Jos, Angeles Cameán, Ana María |
| author_role | author |
| collection | RIO. Repositorio Institucional Olavide |
| description | Cyanotoxins are a large group of noxious metabolites with different chemical structure and mechanisms of action, with a worldwide distribution, producing effects in animals, humans, and crop plants. When cyanotoxin-contaminated waters are used for the irrigation of edible vegetables, humans can be in contact with these toxins through the food chain. In this work, a method for the simultaneous detection of Microcystin-LR (MC-LR), Microcystin-RR (MC-RR), Microcystin-YR (MC-YR), and Cylindrospermopsin (CYN) in lettuce has been optimized and validated, using a dual solid phase extraction (SPE) system for toxin extraction and ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) for analysis. Results showed linear ranges (5–50 ng g1 f.w.), low values for limit of detection (LOD) (0.06–0.42 ng g1 f.w.), and limit of quantification (LOQ) (0.16–0.91 ng g1 f.w.), acceptable recoveries (41–93%), and %RSDIP values for the four toxins. The method proved to be robust for the three variables tested. Finally, it was successfully applied to detect these cyanotoxins in edible vegetables exposed to cyanobacterial extracts under laboratory conditions, and it could be useful for monitoring these toxins in edible vegetables for better exposure estimation in terms of risk assessment. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_2124f0608ea4a9dbfa9cc50406da4b60 |
| institution | Universidad Pablo de Olavide (UPO) |
| instname_str | Universidad Pablo de Olavide (UPO) |
| language | eng |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:rio.upo.es:10433/19837 |
| publishDate | 2018 |
| publishDateSort | 2018 |
| publisher | MDPI |
| publisherStr | MDPI |
| reponame_str | RIO. Repositorio Institucional Olavide |
| spelling | New Method for Simultaneous Determination of Microcystins and Cylindrospermopsin in Vegetable Matrices by SPE-UPLC-MS/MS Diez-Quijada, Leticia Guzmán-Guillén, Remedios Prieto, Ana Isabel Llana Ruíz-Cabello, María Campos, Alexandre Vasconcelos, Vitor Jos, Angeles Cameán, Ana María Microcystins Cylindrospermopsin UPLC-MS/MS Cyanotoxins are a large group of noxious metabolites with different chemical structure and mechanisms of action, with a worldwide distribution, producing effects in animals, humans, and crop plants. When cyanotoxin-contaminated waters are used for the irrigation of edible vegetables, humans can be in contact with these toxins through the food chain. In this work, a method for the simultaneous detection of Microcystin-LR (MC-LR), Microcystin-RR (MC-RR), Microcystin-YR (MC-YR), and Cylindrospermopsin (CYN) in lettuce has been optimized and validated, using a dual solid phase extraction (SPE) system for toxin extraction and ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) for analysis. Results showed linear ranges (5–50 ng g1 f.w.), low values for limit of detection (LOD) (0.06–0.42 ng g1 f.w.), and limit of quantification (LOQ) (0.16–0.91 ng g1 f.w.), acceptable recoveries (41–93%), and %RSDIP values for the four toxins. The method proved to be robust for the three variables tested. Finally, it was successfully applied to detect these cyanotoxins in edible vegetables exposed to cyanobacterial extracts under laboratory conditions, and it could be useful for monitoring these toxins in edible vegetables for better exposure estimation in terms of risk assessment. MDPI https://hdl.handle.net/10433/19837 |
| spellingShingle | New Method for Simultaneous Determination of Microcystins and Cylindrospermopsin in Vegetable Matrices by SPE-UPLC-MS/MS Diez-Quijada, Leticia Microcystins Cylindrospermopsin UPLC-MS/MS |
| title | New Method for Simultaneous Determination of Microcystins and Cylindrospermopsin in Vegetable Matrices by SPE-UPLC-MS/MS |
| title_full | New Method for Simultaneous Determination of Microcystins and Cylindrospermopsin in Vegetable Matrices by SPE-UPLC-MS/MS |
| title_fullStr | New Method for Simultaneous Determination of Microcystins and Cylindrospermopsin in Vegetable Matrices by SPE-UPLC-MS/MS |
| title_full_unstemmed | New Method for Simultaneous Determination of Microcystins and Cylindrospermopsin in Vegetable Matrices by SPE-UPLC-MS/MS |
| title_short | New Method for Simultaneous Determination of Microcystins and Cylindrospermopsin in Vegetable Matrices by SPE-UPLC-MS/MS |
| title_sort | New Method for Simultaneous Determination of Microcystins and Cylindrospermopsin in Vegetable Matrices by SPE-UPLC-MS/MS |
| topic | Microcystins Cylindrospermopsin UPLC-MS/MS |
| topic_facet | Microcystins Cylindrospermopsin UPLC-MS/MS |
| url | https://hdl.handle.net/10433/19837 |