Functional interaction between peroxisome proliferator-activated receptors-alpha and Mef-2C on human carnitine palmitoyltransferase 1beta (CPT1beta) gene activation
Muscle-type carnitine palmitoyltransferase 1 (CPT1β) is considered to be the gene that controls fatty acid mitochondrial β-oxidation. A functional peroxisome proliferator-activated receptor (PPAR) responsive element (PPRE) and a myocite-specific (MEF2) site that binds MEF2A and MEF2C in the promoter...
| Autores: | ; ; ; |
|---|---|
| Tipo de documento: | Artículo |
| Estado: | Published version |
| Fecha de publicación: | 2004 |
| País: | España |
| Institución: | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| Repositorio: | Recercat. Dipósit de la Recerca de Catalunya |
| OAI Identifier: | oai:recercat.cat:2445/48163 |
| Acceso en línea: | https://hdl.handle.net/2445/48163 |
| Nivel de acceso: | Acceso abierto |
| Palabra clave: | Àcids grassos Oxidació fisiològica Receptors nuclears (Bioquímica) Factors de transcripció Transferases Fatty acids Physiological oxidation Nuclear receptors (Biochemistry) Transcription factors |
| _version_ | 1878730545446256640 |
|---|---|
| author | Baldán Aranda, Ángel |
| author2 | Relat Pardo, Joana Marrero González, Pedro F. Haro Bautista, Diego |
| author2_role | author author author |
| author_browse | Baldán Aranda, Ángel Haro Bautista, Diego Marrero González, Pedro F. Relat Pardo, Joana |
| author_facet | Baldán Aranda, Ángel Relat Pardo, Joana Marrero González, Pedro F. Haro Bautista, Diego |
| author_role | author |
| collection | Recercat. Dipósit de la Recerca de Catalunya |
| description | Muscle-type carnitine palmitoyltransferase 1 (CPT1β) is considered to be the gene that controls fatty acid mitochondrial β-oxidation. A functional peroxisome proliferator-activated receptor (PPAR) responsive element (PPRE) and a myocite-specific (MEF2) site that binds MEF2A and MEF2C in the promoter of this gene had been previously identified. We investigated the roles of the PPRE and the MEF2 binding sites and the potential interaction between PPARα and MEF2C regulating the CPT1β gene promoter. Mutation analysis indicated that the MEF2 site contributed to the activation of the CPT1β promoter by PPAR in C2C12 cells. The reporter construct containing the PPRE and the MEF2C site was synergistically activated by co-expression of PPAR, retinoid X receptor (RXR) and MEF2C in non-muscle cells. Moreover, protein-binding assays demonstrated that MEF2C and PPAR specifically bound to one another in vitro. Also for the synergistic activation of the CPT1β gene promoter by MEF2C and PPARα-RXRα, a precise arrangement of its binding sites was essential. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_1e75d95dc099b565b4b873ef6f80ae81 |
| institution | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| instname_str | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:recercat.cat:2445/48163 |
| publishDate | 2004 |
| publishDateSort | 2004 |
| publisher | Oxford University Press |
| publisherStr | Oxford University Press |
| reponame_str | Recercat. Dipósit de la Recerca de Catalunya |
| spelling | Functional interaction between peroxisome proliferator-activated receptors-alpha and Mef-2C on human carnitine palmitoyltransferase 1beta (CPT1beta) gene activation Baldán Aranda, Ángel Relat Pardo, Joana Marrero González, Pedro F. Haro Bautista, Diego Àcids grassos Oxidació fisiològica Receptors nuclears (Bioquímica) Factors de transcripció Transferases Fatty acids Physiological oxidation Nuclear receptors (Biochemistry) Transcription factors Muscle-type carnitine palmitoyltransferase 1 (CPT1β) is considered to be the gene that controls fatty acid mitochondrial β-oxidation. A functional peroxisome proliferator-activated receptor (PPAR) responsive element (PPRE) and a myocite-specific (MEF2) site that binds MEF2A and MEF2C in the promoter of this gene had been previously identified. We investigated the roles of the PPRE and the MEF2 binding sites and the potential interaction between PPARα and MEF2C regulating the CPT1β gene promoter. Mutation analysis indicated that the MEF2 site contributed to the activation of the CPT1β promoter by PPAR in C2C12 cells. The reporter construct containing the PPRE and the MEF2C site was synergistically activated by co-expression of PPAR, retinoid X receptor (RXR) and MEF2C in non-muscle cells. Moreover, protein-binding assays demonstrated that MEF2C and PPAR specifically bound to one another in vitro. Also for the synergistic activation of the CPT1β gene promoter by MEF2C and PPARα-RXRα, a precise arrangement of its binding sites was essential. Oxford University Press https://hdl.handle.net/2445/48163 |
| spellingShingle | Functional interaction between peroxisome proliferator-activated receptors-alpha and Mef-2C on human carnitine palmitoyltransferase 1beta (CPT1beta) gene activation Baldán Aranda, Ángel Àcids grassos Oxidació fisiològica Receptors nuclears (Bioquímica) Factors de transcripció Transferases Fatty acids Physiological oxidation Nuclear receptors (Biochemistry) Transcription factors |
| status_str | publishedVersion |
| title | Functional interaction between peroxisome proliferator-activated receptors-alpha and Mef-2C on human carnitine palmitoyltransferase 1beta (CPT1beta) gene activation |
| title_full | Functional interaction between peroxisome proliferator-activated receptors-alpha and Mef-2C on human carnitine palmitoyltransferase 1beta (CPT1beta) gene activation |
| title_fullStr | Functional interaction between peroxisome proliferator-activated receptors-alpha and Mef-2C on human carnitine palmitoyltransferase 1beta (CPT1beta) gene activation |
| title_full_unstemmed | Functional interaction between peroxisome proliferator-activated receptors-alpha and Mef-2C on human carnitine palmitoyltransferase 1beta (CPT1beta) gene activation |
| title_short | Functional interaction between peroxisome proliferator-activated receptors-alpha and Mef-2C on human carnitine palmitoyltransferase 1beta (CPT1beta) gene activation |
| title_sort | Functional interaction between peroxisome proliferator-activated receptors-alpha and Mef-2C on human carnitine palmitoyltransferase 1beta (CPT1beta) gene activation |
| topic | Àcids grassos Oxidació fisiològica Receptors nuclears (Bioquímica) Factors de transcripció Transferases Fatty acids Physiological oxidation Nuclear receptors (Biochemistry) Transcription factors |
| topic_facet | Àcids grassos Oxidació fisiològica Receptors nuclears (Bioquímica) Factors de transcripció Transferases Fatty acids Physiological oxidation Nuclear receptors (Biochemistry) Transcription factors |
| url | https://hdl.handle.net/2445/48163 |