Tomato PYR/PYL/RCAR ABA receptors show high expression in root, differential sensitivity to the ABA-agonist quinabactin and capability to enhance plant drought resistance
[EN] Abscisic acid (ABA) plays a crucial role in the plant’s response to both biotic and abiotic stress. Sustainable production of food faces several key challenges, particularly the generation of new varieties with improved water use efciency and drought tolerance. Different studies have shown the...
| Autores: | ; ; ; ; ; ; ; ; ; ; ; ; |
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| Tipo de documento: | Artículo |
| Fecha de publicación: | 2014 |
| País: | España |
| Institución: | Universitat Politècnica de València (UPV) |
| Repositorio: | RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
| Lenguaje: | Inglés |
| OAI Identifier: | oai:riunet.upv.es:10251/64399 |
| Acceso en línea: | https://riunet.upv.es/handle/10251/64399 |
| Nivel de acceso: | Acceso abierto |
| Palabra clave: | Abscisic acid (ABA) Drought resistance Tomato ABA receptor Tomato clade A PP2C MICROBIOLOGIA BIOQUIMICA Y BIOLOGIA MOLECULAR |
| _version_ | 1878730442987798528 |
|---|---|
| author | González Guzmán, Miguel |
| author2 | Rodriguez, Lesia Lorenzo Orts, Laura Pons Puig, Clara Sarrion-Perdigones, Alejandro Fernandez, Maria A Peirats Llobet, Marta Forment Millet, José Javier . Moreno Alvero, María Cutler, Sean R. Albert, Armando GRANELL RICHART, ANTONIO|||0000-0003-4266-9581 Rodríguez Egea, Pedro Luís|||0000-0002-5886-9425 |
| author2_role | author author author author author author author author author author author author |
| author_browse | Albert, Armando Cutler, Sean R. Fernandez, Maria A Forment Millet, José Javier . GRANELL RICHART, ANTONIO|||0000-0003-4266-9581 González Guzmán, Miguel Lorenzo Orts, Laura Moreno Alvero, María Peirats Llobet, Marta Pons Puig, Clara Rodriguez, Lesia Rodríguez Egea, Pedro Luís|||0000-0002-5886-9425 Sarrion-Perdigones, Alejandro |
| author_facet | González Guzmán, Miguel Rodriguez, Lesia Lorenzo Orts, Laura Pons Puig, Clara Sarrion-Perdigones, Alejandro Fernandez, Maria A Peirats Llobet, Marta Forment Millet, José Javier . Moreno Alvero, María Cutler, Sean R. Albert, Armando GRANELL RICHART, ANTONIO|||0000-0003-4266-9581 Rodríguez Egea, Pedro Luís|||0000-0002-5886-9425 |
| author_role | author |
| collection | RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
| description | [EN] Abscisic acid (ABA) plays a crucial role in the plant’s response to both biotic and abiotic stress. Sustainable production of food faces several key challenges, particularly the generation of new varieties with improved water use efciency and drought tolerance. Different studies have shown the potential applications of Arabidopsis PYR/PYL/ RCAR ABA receptors to enhance plant drought resistance. Consequently the functional characterization of orthologous genes in crops holds promise for agriculture. The full set of tomato (Solanum lycopersicum) PYR/PYL/RCAR ABA receptors have been identied here. From the 15 putative tomato ABA receptors, 14 of them could be grouped in three subfamilies that correlated well with corresponding Arabidopsis subfamilies. High levels of expression of PYR/ PYL/RCAR genes was found in tomato root, and some genes showed predominant expression in leaf and fruit tissues. Functional characterization of tomato receptors was performed through interaction assays with Arabidopsis and tomato clade A protein phosphatase type 2Cs (PP2Cs) as well as phosphatase inhibition studies. Tomato receptors were able to inhibit the activity of clade A PP2Cs differentially in an ABA-dependent manner, and at least three receptors were sensitive to the ABA agonist quinabactin, which inhibited tomato seed germination. Indeed, the chemical activation of ABA signalling induced by quinabactin was able to activate stress-responsive genes. Both dimeric and monomeric tomato receptors were functional in Arabidopsis plant cells, but only overexpression of monomeric-type receptors conferred enhanced drought resistance. In summary, gene expression analyses, and chemical and transgenic approaches revealed distinct properties of tomato PYR/PYL/RCAR ABA receptors that might have biotechnological implications. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_1c5f8e3d7394b2e9adbfee5675035ccf |
| institution | Universitat Politècnica de València (UPV) |
| instname_str | Universitat Politècnica de València (UPV) |
| language | eng |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:riunet.upv.es:10251/64399 |
| publishDate | 2014 |
| publishDateSort | 2014 |
| publisher | Oxford University Press |
| publisherStr | Oxford University Press |
| reponame_str | RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
| spelling | Tomato PYR/PYL/RCAR ABA receptors show high expression in root, differential sensitivity to the ABA-agonist quinabactin and capability to enhance plant drought resistance González Guzmán, Miguel Rodriguez, Lesia Lorenzo Orts, Laura Pons Puig, Clara Sarrion-Perdigones, Alejandro Fernandez, Maria A Peirats Llobet, Marta Forment Millet, José Javier . Moreno Alvero, María Cutler, Sean R. Albert, Armando GRANELL RICHART, ANTONIO|||0000-0003-4266-9581 Rodríguez Egea, Pedro Luís|||0000-0002-5886-9425 Abscisic acid (ABA) Drought resistance Tomato ABA receptor Tomato clade A PP2C MICROBIOLOGIA BIOQUIMICA Y BIOLOGIA MOLECULAR [EN] Abscisic acid (ABA) plays a crucial role in the plant’s response to both biotic and abiotic stress. Sustainable production of food faces several key challenges, particularly the generation of new varieties with improved water use efciency and drought tolerance. Different studies have shown the potential applications of Arabidopsis PYR/PYL/ RCAR ABA receptors to enhance plant drought resistance. Consequently the functional characterization of orthologous genes in crops holds promise for agriculture. The full set of tomato (Solanum lycopersicum) PYR/PYL/RCAR ABA receptors have been identied here. From the 15 putative tomato ABA receptors, 14 of them could be grouped in three subfamilies that correlated well with corresponding Arabidopsis subfamilies. High levels of expression of PYR/ PYL/RCAR genes was found in tomato root, and some genes showed predominant expression in leaf and fruit tissues. Functional characterization of tomato receptors was performed through interaction assays with Arabidopsis and tomato clade A protein phosphatase type 2Cs (PP2Cs) as well as phosphatase inhibition studies. Tomato receptors were able to inhibit the activity of clade A PP2Cs differentially in an ABA-dependent manner, and at least three receptors were sensitive to the ABA agonist quinabactin, which inhibited tomato seed germination. Indeed, the chemical activation of ABA signalling induced by quinabactin was able to activate stress-responsive genes. Both dimeric and monomeric tomato receptors were functional in Arabidopsis plant cells, but only overexpression of monomeric-type receptors conferred enhanced drought resistance. In summary, gene expression analyses, and chemical and transgenic approaches revealed distinct properties of tomato PYR/PYL/RCAR ABA receptors that might have biotechnological implications. This work was supported by the Ministerio de Ciencia e Innovacion, Fondo Europeo de Desarrollo Regional and Consejo Superior de Investigaciones Cientificas (grants BIO2011-23446 to PLR, BFU2011-25384 to AA, and Fontagro and COST1106 for networking activities to AG); fellowships to LR and MP; and a Juan de la Cierva contract to MGG. Cristina Martinez-Andujar and the Bioinformatics Core Service of the IBMCP are acknowledged for the SlEF1a marker and help in bioinformatics analyses, respectively. Oxford University Press https://riunet.upv.es/handle/10251/64399 |
| spellingShingle | Tomato PYR/PYL/RCAR ABA receptors show high expression in root, differential sensitivity to the ABA-agonist quinabactin and capability to enhance plant drought resistance González Guzmán, Miguel Abscisic acid (ABA) Drought resistance Tomato ABA receptor Tomato clade A PP2C MICROBIOLOGIA BIOQUIMICA Y BIOLOGIA MOLECULAR |
| title | Tomato PYR/PYL/RCAR ABA receptors show high expression in root, differential sensitivity to the ABA-agonist quinabactin and capability to enhance plant drought resistance |
| title_full | Tomato PYR/PYL/RCAR ABA receptors show high expression in root, differential sensitivity to the ABA-agonist quinabactin and capability to enhance plant drought resistance |
| title_fullStr | Tomato PYR/PYL/RCAR ABA receptors show high expression in root, differential sensitivity to the ABA-agonist quinabactin and capability to enhance plant drought resistance |
| title_full_unstemmed | Tomato PYR/PYL/RCAR ABA receptors show high expression in root, differential sensitivity to the ABA-agonist quinabactin and capability to enhance plant drought resistance |
| title_short | Tomato PYR/PYL/RCAR ABA receptors show high expression in root, differential sensitivity to the ABA-agonist quinabactin and capability to enhance plant drought resistance |
| title_sort | Tomato PYR/PYL/RCAR ABA receptors show high expression in root, differential sensitivity to the ABA-agonist quinabactin and capability to enhance plant drought resistance |
| topic | Abscisic acid (ABA) Drought resistance Tomato ABA receptor Tomato clade A PP2C MICROBIOLOGIA BIOQUIMICA Y BIOLOGIA MOLECULAR |
| topic_facet | Abscisic acid (ABA) Drought resistance Tomato ABA receptor Tomato clade A PP2C MICROBIOLOGIA BIOQUIMICA Y BIOLOGIA MOLECULAR |
| url | https://riunet.upv.es/handle/10251/64399 |