DNA methylation biomarkers of myocardial infarction and cardiovascular disease
Background: The epigenetic landscape underlying cardiovascular disease (CVD) is not completely understood and the clinical value of the identified biomarkers is still limited. We aimed to identify differentially methylated loci associated with acute myocardial infarction (AMI) and assess their valid...
| Authors: | ; ; ; ; ; ; ; ; |
|---|---|
| Format: | Article |
| Status: | Published version |
| Publication date: | 2021 |
| Country: | España |
| Institution: | Universitat Pompeu Fabra |
| Repository: | Repositorio Digital de la UPF |
| OAI Identifier: | oai:repositori.upf.edu:10230/48409 |
| Online Access: | http://hdl.handle.net/10230/48409 http://dx.doi.org/10.1186/s13148-021-01078-6 |
| Access Level: | Open access |
| Keyword: | Cardiovascular disease DNA methylation Epigenome-wide association study Myocardial infarction Predictive biomarkers |
| _version_ | 1878730335426969600 |
|---|---|
| author | Fernández Sanlés, Alba, 1988- |
| author2 | Sayols Baixeras, Sergi, 1988- Subirana Cachinero, Isaac Sentí Clapés, Mariano Pérez-Fernández, Silvia Castro de Moura, Manuel Esteller, Manel Marrugat, Jaume, 1954- Elosua Llanos, Roberto |
| author2_role | author author author author author author author author |
| author_browse | Castro de Moura, Manuel Elosua Llanos, Roberto Esteller, Manel Fernández Sanlés, Alba, 1988- Marrugat, Jaume, 1954- Pérez-Fernández, Silvia Sayols Baixeras, Sergi, 1988- Sentí Clapés, Mariano Subirana Cachinero, Isaac |
| author_facet | Fernández Sanlés, Alba, 1988- Sayols Baixeras, Sergi, 1988- Subirana Cachinero, Isaac Sentí Clapés, Mariano Pérez-Fernández, Silvia Castro de Moura, Manuel Esteller, Manel Marrugat, Jaume, 1954- Elosua Llanos, Roberto |
| author_role | author |
| collection | Repositorio Digital de la UPF |
| description | Background: The epigenetic landscape underlying cardiovascular disease (CVD) is not completely understood and the clinical value of the identified biomarkers is still limited. We aimed to identify differentially methylated loci associated with acute myocardial infarction (AMI) and assess their validity as predictive and causal biomarkers. Results: We designed a case-control, two-stage, epigenome-wide association study on AMI (ndiscovery = 391, nvalidation = 204). DNA methylation was assessed using the Infinium MethylationEPIC BeadChip. We performed a fixed-effects meta-analysis of the two samples. 34 CpGs were associated with AMI. Only 12 of them were available in two independent cohort studies (n ~ 1800 and n ~ 2500) with incident coronary and cardiovascular disease (CHD and CVD, respectively). The Infinium HumanMethylation450 BeadChip was used in those two studies. Four of the 12 CpGs were validated in association with incident CHD: AHRR-mapping cg05575921, PTCD2-mapping cg25769469, intergenic cg21566642 and MPO-mapping cg04988978. We then assessed whether methylation risk scores based on those CpGs improved the predictive capacity of the Framingham risk function, but they did not. Finally, we aimed to study the causality of those associations using a Mendelian randomization approach but only one of the CpGs had a genetic influence and therefore the results were not conclusive. Conclusions: We have identified 34 CpGs related to AMI. These loci highlight the relevance of smoking, lipid metabolism, and inflammation in the biological mechanisms related to AMI. Four were additionally associated with incident CHD and CVD but did not provide additional predictive information. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_1a3c384d99d4b8d5b2a00a70b8221fa9 |
| institution | Universitat Pompeu Fabra |
| instname_str | Universitat Pompeu Fabra |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:repositori.upf.edu:10230/48409 |
| publishDate | 2021 |
| publishDateSort | 2021 |
| publisher | BioMed Central |
| publisherStr | BioMed Central |
| reponame_str | Repositorio Digital de la UPF |
| spelling | DNA methylation biomarkers of myocardial infarction and cardiovascular disease Fernández Sanlés, Alba, 1988- Sayols Baixeras, Sergi, 1988- Subirana Cachinero, Isaac Sentí Clapés, Mariano Pérez-Fernández, Silvia Castro de Moura, Manuel Esteller, Manel Marrugat, Jaume, 1954- Elosua Llanos, Roberto Cardiovascular disease DNA methylation Epigenome-wide association study Myocardial infarction Predictive biomarkers Background: The epigenetic landscape underlying cardiovascular disease (CVD) is not completely understood and the clinical value of the identified biomarkers is still limited. We aimed to identify differentially methylated loci associated with acute myocardial infarction (AMI) and assess their validity as predictive and causal biomarkers. Results: We designed a case-control, two-stage, epigenome-wide association study on AMI (ndiscovery = 391, nvalidation = 204). DNA methylation was assessed using the Infinium MethylationEPIC BeadChip. We performed a fixed-effects meta-analysis of the two samples. 34 CpGs were associated with AMI. Only 12 of them were available in two independent cohort studies (n ~ 1800 and n ~ 2500) with incident coronary and cardiovascular disease (CHD and CVD, respectively). The Infinium HumanMethylation450 BeadChip was used in those two studies. Four of the 12 CpGs were validated in association with incident CHD: AHRR-mapping cg05575921, PTCD2-mapping cg25769469, intergenic cg21566642 and MPO-mapping cg04988978. We then assessed whether methylation risk scores based on those CpGs improved the predictive capacity of the Framingham risk function, but they did not. Finally, we aimed to study the causality of those associations using a Mendelian randomization approach but only one of the CpGs had a genetic influence and therefore the results were not conclusive. Conclusions: We have identified 34 CpGs related to AMI. These loci highlight the relevance of smoking, lipid metabolism, and inflammation in the biological mechanisms related to AMI. Four were additionally associated with incident CHD and CVD but did not provide additional predictive information. BioMed Central http://hdl.handle.net/10230/48409 http://dx.doi.org/10.1186/s13148-021-01078-6 |
| spellingShingle | DNA methylation biomarkers of myocardial infarction and cardiovascular disease Fernández Sanlés, Alba, 1988- Cardiovascular disease DNA methylation Epigenome-wide association study Myocardial infarction Predictive biomarkers |
| status_str | publishedVersion |
| title | DNA methylation biomarkers of myocardial infarction and cardiovascular disease |
| title_full | DNA methylation biomarkers of myocardial infarction and cardiovascular disease |
| title_fullStr | DNA methylation biomarkers of myocardial infarction and cardiovascular disease |
| title_full_unstemmed | DNA methylation biomarkers of myocardial infarction and cardiovascular disease |
| title_short | DNA methylation biomarkers of myocardial infarction and cardiovascular disease |
| title_sort | DNA methylation biomarkers of myocardial infarction and cardiovascular disease |
| topic | Cardiovascular disease DNA methylation Epigenome-wide association study Myocardial infarction Predictive biomarkers |
| topic_facet | Cardiovascular disease DNA methylation Epigenome-wide association study Myocardial infarction Predictive biomarkers |
| url | http://hdl.handle.net/10230/48409 http://dx.doi.org/10.1186/s13148-021-01078-6 |