Gallium and silver-doped titanium surfaces provide enhanced osteogenesis, reduce bone resorption and prevent bacterial infection in co-culture
Bacterial infection remains a significant problem associated with orthopaedic surgeries leading to surgical site infection (SSI). This unmet medical need can become an even greater complication when surgery is due to malignant bone tumor. In the present study, we evaluated in vitro titanium (Ti) imp...
| Authors: | ; ; ; ; ; ; ; |
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| Format: | Article |
| Status: | Published version |
| Publication date: | 2024 |
| Country: | España |
| Institution: | Fundació Sant Joan de Déu |
| Repository: | r-FSJD. Repositorio Institucional de Producción Científica de la Fundació Sant Joan de Déu |
| OAI Identifier: | oai:fsjd.fundanetsuite.com:p26125 |
| Online Access: | https://fsjd.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=26125 |
| Access Level: | Open access |
| Keyword: | Silver Gallium Titanium Bone resorption Antibacterial |
| _version_ | 1878730320096788480 |
|---|---|
| author | Piñera-Avellaneda D |
| author2 | Buxadera-Palomero J Delint RC Dalby MJ Burgess KV Ginebra MP Rupérez E Manero JM |
| author2_role | author author author author author author author |
| author_browse | Burgess KV Buxadera-Palomero J Dalby MJ Delint RC Ginebra MP Manero JM Piñera-Avellaneda D Rupérez E |
| author_facet | Piñera-Avellaneda D Buxadera-Palomero J Delint RC Dalby MJ Burgess KV Ginebra MP Rupérez E Manero JM |
| author_role | author |
| collection | r-FSJD. Repositorio Institucional de Producción Científica de la Fundació Sant Joan de Déu |
| description | Bacterial infection remains a significant problem associated with orthopaedic surgeries leading to surgical site infection (SSI). This unmet medical need can become an even greater complication when surgery is due to malignant bone tumor. In the present study, we evaluated in vitro titanium (Ti) implants subjected to gallium (Ga) and silver (Ag)-doped thermochemical treatment as strategy to prevent SSI and improve osteointegration in bone defects caused by diseases such as osteoporosis, bone tumor, or bone metastasis. Firstly, as Ga has been reported to be an osteoinductive and anti-resorptive agent, its performance in the mixture was proved by studying human mesenchymal stem cells (hMSC) and pre-osteoclasts (RAW264.7) behaviour. Then, the antibacterial potential provided by Ag was assessed by resembling "The Race for the Surface" between hMSC and Pseudomonas aeruginosa in two co-culture methods. Moreover, the presence of quorum sensing molecules in the co-culture was evaluated. The results highlighted the suitability of the mixture to induce osteodifferentiation and reduce osteoclastogenesis in vitro . Furthermore, the GaAg surface promoted strong survival rate and retained osteoinduction potential of hMSCs even after bacterial inoculation. Therefore, GaAg-modified titanium may be an ideal candidate to repair bone defects caused by excessive bone resorption, in addition to preventing SSI. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_19f3b720a942e676ae304de55c19b3bf |
| institution | Fundació Sant Joan de Déu |
| instname_str | Fundació Sant Joan de Déu |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:fsjd.fundanetsuite.com:p26125 |
| publishDate | 2024 |
| publishDateSort | 2024 |
| publisher | ELSEVIER SCI LTD |
| publisherStr | ELSEVIER SCI LTD |
| reponame_str | r-FSJD. Repositorio Institucional de Producción Científica de la Fundació Sant Joan de Déu |
| spelling | Gallium and silver-doped titanium surfaces provide enhanced osteogenesis, reduce bone resorption and prevent bacterial infection in co-culture Piñera-Avellaneda D Buxadera-Palomero J Delint RC Dalby MJ Burgess KV Ginebra MP Rupérez E Manero JM Silver Gallium Titanium Bone resorption Antibacterial Bacterial infection remains a significant problem associated with orthopaedic surgeries leading to surgical site infection (SSI). This unmet medical need can become an even greater complication when surgery is due to malignant bone tumor. In the present study, we evaluated in vitro titanium (Ti) implants subjected to gallium (Ga) and silver (Ag)-doped thermochemical treatment as strategy to prevent SSI and improve osteointegration in bone defects caused by diseases such as osteoporosis, bone tumor, or bone metastasis. Firstly, as Ga has been reported to be an osteoinductive and anti-resorptive agent, its performance in the mixture was proved by studying human mesenchymal stem cells (hMSC) and pre-osteoclasts (RAW264.7) behaviour. Then, the antibacterial potential provided by Ag was assessed by resembling "The Race for the Surface" between hMSC and Pseudomonas aeruginosa in two co-culture methods. Moreover, the presence of quorum sensing molecules in the co-culture was evaluated. The results highlighted the suitability of the mixture to induce osteodifferentiation and reduce osteoclastogenesis in vitro . Furthermore, the GaAg surface promoted strong survival rate and retained osteoinduction potential of hMSCs even after bacterial inoculation. Therefore, GaAg-modified titanium may be an ideal candidate to repair bone defects caused by excessive bone resorption, in addition to preventing SSI. ELSEVIER SCI LTD https://fsjd.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=26125 |
| spellingShingle | Gallium and silver-doped titanium surfaces provide enhanced osteogenesis, reduce bone resorption and prevent bacterial infection in co-culture Piñera-Avellaneda D Silver Gallium Titanium Bone resorption Antibacterial |
| status_str | publishedVersion |
| title | Gallium and silver-doped titanium surfaces provide enhanced osteogenesis, reduce bone resorption and prevent bacterial infection in co-culture |
| title_full | Gallium and silver-doped titanium surfaces provide enhanced osteogenesis, reduce bone resorption and prevent bacterial infection in co-culture |
| title_fullStr | Gallium and silver-doped titanium surfaces provide enhanced osteogenesis, reduce bone resorption and prevent bacterial infection in co-culture |
| title_full_unstemmed | Gallium and silver-doped titanium surfaces provide enhanced osteogenesis, reduce bone resorption and prevent bacterial infection in co-culture |
| title_short | Gallium and silver-doped titanium surfaces provide enhanced osteogenesis, reduce bone resorption and prevent bacterial infection in co-culture |
| title_sort | Gallium and silver-doped titanium surfaces provide enhanced osteogenesis, reduce bone resorption and prevent bacterial infection in co-culture |
| topic | Silver Gallium Titanium Bone resorption Antibacterial |
| topic_facet | Silver Gallium Titanium Bone resorption Antibacterial |
| url | https://fsjd.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=26125 |