Why not glycine electrochemical biosensors?
Glycine monitoring is gaining importance as a biomarker in clinical analysis due to its involvement in multiple physiological functions, which results in glycine being one of the most analyzed biomolecules for diagnostics. This growing demand requires faster and more reliable, while affordable, anal...
| Authors: | ; ; ; ; |
|---|---|
| Format: | Article |
| Status: | Published version |
| Publication date: | 2020 |
| Country: | España |
| Institution: | Universidad de Barcelona |
| Repository: | Dipòsit Digital de la UB |
| OAI Identifier: | oai:diposit.ub.edu:2445/173834 |
| Online Access: | https://hdl.handle.net/2445/173834 |
| Access Level: | Open access |
| Keyword: | Electroquímica Biosensors Assistència sanitària Electrochemistry Medical care |
| _version_ | 1878729798353682432 |
|---|---|
| author | Pérez Ràfols, Clara |
| author2 | Liu, Yujie Wang, Qianyu Cuartero, María Crespo, Gastón A. |
| author2_role | author author author author |
| author_browse | Crespo, Gastón A. Cuartero, María Liu, Yujie Pérez Ràfols, Clara Wang, Qianyu |
| author_facet | Pérez Ràfols, Clara Liu, Yujie Wang, Qianyu Cuartero, María Crespo, Gastón A. |
| author_role | author |
| collection | Dipòsit Digital de la UB |
| description | Glycine monitoring is gaining importance as a biomarker in clinical analysis due to its involvement in multiple physiological functions, which results in glycine being one of the most analyzed biomolecules for diagnostics. This growing demand requires faster and more reliable, while affordable, analytical methods that can replace the current gold standard for glycine detection, which is based on sample extraction with subsequent use of liquid chromatography or fluorometric kits for its quantification in centralized laboratories. This work discusses electrochemical sensors and biosensors as an alternative option, focusing on their potential application for glycine determination in blood, urine, and cerebrospinal fluid, the three most widely used matrices for glycine analysis with clinical meaning. For electrochemical sensors, voltammetry/amperometry is the preferred readout (10 of the 13 papers collected in this review) and metal-based redox mediator modification is the predominant approach for electrode fabrication (11 of the 13 papers). However, none of the reported electrochemical sensors fulfill the requirements for direct analysis of biological fluids, most of them lacking appropriate selectivity, linear range of response, and/or capability of measuring at physiological conditions. Enhanced selectivity has been recently reported using biosensors (with an enzyme element in the electrode design), although this is still a very incipient approach. Currently, despite the benefits of electrochemistry, only optical biosensors have been successfully reported for glycine detection and, from all the inspected works, it is clear that bioengineering efforts will play a key role in the embellishment of selectivity and storage stability of the sensing element in the sensor. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_0f3f8b9fdff003fdf3bd16f3bd653d84 |
| institution | Universidad de Barcelona |
| instname_str | Universidad de Barcelona |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:diposit.ub.edu:2445/173834 |
| publishDate | 2020 |
| publishDateSort | 2020 |
| publisher | MDPI |
| publisherStr | MDPI |
| reponame_str | Dipòsit Digital de la UB |
| spelling | Why not glycine electrochemical biosensors? Pérez Ràfols, Clara Liu, Yujie Wang, Qianyu Cuartero, María Crespo, Gastón A. Electroquímica Biosensors Assistència sanitària Electrochemistry Medical care Glycine monitoring is gaining importance as a biomarker in clinical analysis due to its involvement in multiple physiological functions, which results in glycine being one of the most analyzed biomolecules for diagnostics. This growing demand requires faster and more reliable, while affordable, analytical methods that can replace the current gold standard for glycine detection, which is based on sample extraction with subsequent use of liquid chromatography or fluorometric kits for its quantification in centralized laboratories. This work discusses electrochemical sensors and biosensors as an alternative option, focusing on their potential application for glycine determination in blood, urine, and cerebrospinal fluid, the three most widely used matrices for glycine analysis with clinical meaning. For electrochemical sensors, voltammetry/amperometry is the preferred readout (10 of the 13 papers collected in this review) and metal-based redox mediator modification is the predominant approach for electrode fabrication (11 of the 13 papers). However, none of the reported electrochemical sensors fulfill the requirements for direct analysis of biological fluids, most of them lacking appropriate selectivity, linear range of response, and/or capability of measuring at physiological conditions. Enhanced selectivity has been recently reported using biosensors (with an enzyme element in the electrode design), although this is still a very incipient approach. Currently, despite the benefits of electrochemistry, only optical biosensors have been successfully reported for glycine detection and, from all the inspected works, it is clear that bioengineering efforts will play a key role in the embellishment of selectivity and storage stability of the sensing element in the sensor. MDPI https://hdl.handle.net/2445/173834 |
| spellingShingle | Why not glycine electrochemical biosensors? Pérez Ràfols, Clara Electroquímica Biosensors Assistència sanitària Electrochemistry Medical care |
| status_str | publishedVersion |
| title | Why not glycine electrochemical biosensors? |
| title_full | Why not glycine electrochemical biosensors? |
| title_fullStr | Why not glycine electrochemical biosensors? |
| title_full_unstemmed | Why not glycine electrochemical biosensors? |
| title_short | Why not glycine electrochemical biosensors? |
| title_sort | Why not glycine electrochemical biosensors? |
| topic | Electroquímica Biosensors Assistència sanitària Electrochemistry Medical care |
| topic_facet | Electroquímica Biosensors Assistència sanitària Electrochemistry Medical care |
| url | https://hdl.handle.net/2445/173834 |