Identification of Single-Nucleotide Polymorphism in the Progesterone Receptor Gene and Its Association With Reproductive Traits in Rabbits
[EN] A total of 598 F-2 does from a cross between the high and low lines selected divergently for uterine capacity during 10 generations were used in a candidate gene analysis. The presence of major genes affecting the number of implanted embryos and uterine capacity has been suggested in lines dive...
| Authors: | ; ; ; ; ; ; |
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| Format: | Article |
| Publication date: | 2008 |
| Country: | España |
| Institution: | Universitat Politècnica de València (UPV) |
| Repository: | RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
| Language: | English |
| OAI Identifier: | oai:riunet.upv.es:10251/202754 |
| Online Access: | https://riunet.upv.es/handle/10251/202754 |
| Access Level: | Open access |
| Keyword: | Rabbits F2 Progesterone receptor gene Litter size Gene polymorphisms PRODUCCION ANIMAL GENETICA |
| _version_ | 1878729722357088256 |
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| author | Peiró Barber, Rosa Mª|||0000-0002-3009-2343 |
| author2 | Santacreu, María-Antonia|||0000-0002-6847-9551 Blasco Mateu, Agustín|||0000-0003-3558-0810 Merchán, M. Argente, M. J. García, M. L. Folch, J. M. |
| author2_role | author author author author author author |
| author_browse | Argente, M. J. Blasco Mateu, Agustín|||0000-0003-3558-0810 Folch, J. M. García, M. L. Merchán, M. Peiró Barber, Rosa Mª|||0000-0002-3009-2343 Santacreu, María-Antonia|||0000-0002-6847-9551 |
| author_facet | Peiró Barber, Rosa Mª|||0000-0002-3009-2343 Santacreu, María-Antonia|||0000-0002-6847-9551 Blasco Mateu, Agustín|||0000-0003-3558-0810 Merchán, M. Argente, M. J. García, M. L. Folch, J. M. |
| author_role | author |
| collection | RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
| description | [EN] A total of 598 F-2 does from a cross between the high and low lines selected divergently for uterine capacity during 10 generations were used in a candidate gene analysis. The presence of major genes affecting the number of implanted embryos and uterine capacity has been suggested in lines divergently selected for Uterine capacity. Uterine capacity is it main component of litter size. The progesterone receptor gene was tested as a candidate gene to determine whether polymorphisms explain differences in litter size and its components. Fragments of the promoter region and exons 1-8 were amplified and sequenced. One SNP was found in the promoter region, 2464G > A, three SNPs in the 5'-UTR exon 1, and a silence SNP in exon 7. The first four SNPs were segregated in two haplotypes. The allele G found in the promoter region was found in 75% of the high-line parental animals and in 29% of the low-line parental animals. The GG genotype had 0.5 kits and 0.5 implanted embryos more than the AA genotype. At 48 hr of gestation, the difference in early embryo survival and embryonic stage of development. was small. However, at 72 hr of gestation, the GG genotype had 0.36 embryos more than the AA genotype and also had a more advanced embryonic stage of development, showing a lower percentage of compacted morulae and a higher percentage of blastocysts. The difference in litter size between the GG and CA genotypes was similar to the difference found between homozygote genotypes; however, differences in implanted embryos, early embryo survival, and embryo development were not detected between the GG and GA genotypes. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_0da64bcd68167166b65d771690b8cbbc |
| institution | Universitat Politècnica de València (UPV) |
| instname_str | Universitat Politècnica de València (UPV) |
| language | eng |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:riunet.upv.es:10251/202754 |
| publishDate | 2008 |
| publishDateSort | 2008 |
| publisher | The Genetics Society of America |
| publisherStr | The Genetics Society of America |
| reponame_str | RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
| spelling | Identification of Single-Nucleotide Polymorphism in the Progesterone Receptor Gene and Its Association With Reproductive Traits in Rabbits Peiró Barber, Rosa Mª|||0000-0002-3009-2343 Santacreu, María-Antonia|||0000-0002-6847-9551 Blasco Mateu, Agustín|||0000-0003-3558-0810 Merchán, M. Argente, M. J. García, M. L. Folch, J. M. Rabbits F2 Progesterone receptor gene Litter size Gene polymorphisms PRODUCCION ANIMAL GENETICA [EN] A total of 598 F-2 does from a cross between the high and low lines selected divergently for uterine capacity during 10 generations were used in a candidate gene analysis. The presence of major genes affecting the number of implanted embryos and uterine capacity has been suggested in lines divergently selected for Uterine capacity. Uterine capacity is it main component of litter size. The progesterone receptor gene was tested as a candidate gene to determine whether polymorphisms explain differences in litter size and its components. Fragments of the promoter region and exons 1-8 were amplified and sequenced. One SNP was found in the promoter region, 2464G > A, three SNPs in the 5'-UTR exon 1, and a silence SNP in exon 7. The first four SNPs were segregated in two haplotypes. The allele G found in the promoter region was found in 75% of the high-line parental animals and in 29% of the low-line parental animals. The GG genotype had 0.5 kits and 0.5 implanted embryos more than the AA genotype. At 48 hr of gestation, the difference in early embryo survival and embryonic stage of development. was small. However, at 72 hr of gestation, the GG genotype had 0.36 embryos more than the AA genotype and also had a more advanced embryonic stage of development, showing a lower percentage of compacted morulae and a higher percentage of blastocysts. The difference in litter size between the GG and CA genotypes was similar to the difference found between homozygote genotypes; however, differences in implanted embryos, early embryo survival, and embryo development were not detected between the GG and GA genotypes. The authors are grateful for the excellent assistance provided by R. Muelas and to W. Mekkawi for his software. This study was funded by projects by the Comision Interministerial de Ciencia y Tecnologia AGL2001-3068 and AGL2005-07624. M. Merchan acknowledges a Formacion de Personal Investigador (FPI) grant from the Ministerio de Ciencia y Tecnologia (MCYT) and R. Peiro an FPI grant from Oficinade Ciencia y Tecnologia de la Generalitat Valenciana (OCYT). The Genetics Society of America https://riunet.upv.es/handle/10251/202754 |
| spellingShingle | Identification of Single-Nucleotide Polymorphism in the Progesterone Receptor Gene and Its Association With Reproductive Traits in Rabbits Peiró Barber, Rosa Mª|||0000-0002-3009-2343 Rabbits F2 Progesterone receptor gene Litter size Gene polymorphisms PRODUCCION ANIMAL GENETICA |
| title | Identification of Single-Nucleotide Polymorphism in the Progesterone Receptor Gene and Its Association With Reproductive Traits in Rabbits |
| title_full | Identification of Single-Nucleotide Polymorphism in the Progesterone Receptor Gene and Its Association With Reproductive Traits in Rabbits |
| title_fullStr | Identification of Single-Nucleotide Polymorphism in the Progesterone Receptor Gene and Its Association With Reproductive Traits in Rabbits |
| title_full_unstemmed | Identification of Single-Nucleotide Polymorphism in the Progesterone Receptor Gene and Its Association With Reproductive Traits in Rabbits |
| title_short | Identification of Single-Nucleotide Polymorphism in the Progesterone Receptor Gene and Its Association With Reproductive Traits in Rabbits |
| title_sort | Identification of Single-Nucleotide Polymorphism in the Progesterone Receptor Gene and Its Association With Reproductive Traits in Rabbits |
| topic | Rabbits F2 Progesterone receptor gene Litter size Gene polymorphisms PRODUCCION ANIMAL GENETICA |
| topic_facet | Rabbits F2 Progesterone receptor gene Litter size Gene polymorphisms PRODUCCION ANIMAL GENETICA |
| url | https://riunet.upv.es/handle/10251/202754 |