Impact of sperm DNA damage and oocyte-repairing capacity on trout development
Palabras clave extraídas del título
| Autores: | ; ; ; |
|---|---|
| Tipo de documento: | Artículo |
| Estado: | Published version |
| Fecha de publicación: | 2016 |
| País: | España |
| Institución: | Ajuntament de Barcelona |
| Repositorio: | BULERIA. Repositorio Institucional de la Universidad de León |
| OAI Identifier: | oai:buleria.unileon.es:10612/18707 |
| Acceso en línea: | https://rep.bioscientifica.com/view/journals/rep/152/1/57.xml https://hdl.handle.net/10612/18707 |
| Nivel de acceso: | Acceso abierto |
| Palabra clave: | Biología Sperm DNA damage Oocyte repairing Trout development 2407 Biología Celular 3104.11 Reproducción 2401.07 Embriología Animal |
| _version_ | 1878729687734157312 |
|---|---|
| author | Fernández Díez, Cristina |
| author2 | González Rojo, Silvia Lombó Alonso, Marta Herráez Ortega, María Paz |
| author2_role | author author author |
| author_browse | Fernández Díez, Cristina González Rojo, Silvia Herráez Ortega, María Paz Lombó Alonso, Marta |
| author_facet | Fernández Díez, Cristina González Rojo, Silvia Lombó Alonso, Marta Herráez Ortega, María Paz |
| author_role | author |
| collection | BULERIA. Repositorio Institucional de la Universidad de León |
| description | Palabras clave extraídas del título |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_0cf2f2b3ff3e7ceae3c4c2cc4668ffb8 |
| institution | Ajuntament de Barcelona |
| instname_str | Ajuntament de Barcelona |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:buleria.unileon.es:10612/18707 |
| publishDate | 2016 |
| publishDateSort | 2016 |
| publisher | Society for Reproduction and Fertility |
| publisherStr | Society for Reproduction and Fertility |
| reponame_str | BULERIA. Repositorio Institucional de la Universidad de León |
| spelling | Impact of sperm DNA damage and oocyte-repairing capacity on trout development Fernández Díez, Cristina González Rojo, Silvia Lombó Alonso, Marta Herráez Ortega, María Paz Biología Sperm DNA damage Oocyte repairing Trout development 2407 Biología Celular 3104.11 Reproducción 2401.07 Embriología Animal Palabras clave extraídas del título [EN] Zygotic repair of paternal DNA is essential during embryo development. In spite of the interest devoted to sperm DNA damage, its combined effect with defect-repairing oocytes has not been analyzed. Modification of the breeding season is a common practice in aquaculture. This practice reduces developmental success and could affect the both factors: sperm DNA integrity and oocyte repair capacity. To evaluate the maternal role, we analyzed the progeny outcome after fertilizing in-season trout oocytes with untreated and with UV-irradiated sperm. We also analyzed the offspring obtained out of season with untreated sperm. The analysis of the number of lesions in 4 sperm nuclear genes revealed an increase of 1.22–11.18 lesions/10 kb in out-of-season sperm, similar to that obtained after sperm UV irradiation (400 µW/cm25 min). Gene expression showed in out-of-season oocytes the overexpression of repair genes (ogg1, ung, lig3, rad1) and downregulation of tp53, indicating an enhanced repairing activity and reduced capacity to arrest development upon damage. The analysis of the progeny in out-of-season embryos revealed a similar profile tolerant to DNA damage, leading to a much lower apoptotic activity at organogenesis, lower hatching rates and increased rate of malformations. The effects were milder in descendants from in-season-irradiated sperm, showing an enhanced repairing activity at epibolia. Results point out the importance of the repairing machinery provided by the oocyte and show how susceptible it is to environmental changes. Transcripts related to DNA damage signalization and repair could be used as markers of oocyte quality SI This work was supported by the Spanish Ministry of Economy and Competitiveness (project AGL2011-27787) Society for Reproduction and Fertility https://rep.bioscientifica.com/view/journals/rep/152/1/57.xml https://hdl.handle.net/10612/18707 |
| spellingShingle | Impact of sperm DNA damage and oocyte-repairing capacity on trout development Fernández Díez, Cristina Biología Sperm DNA damage Oocyte repairing Trout development 2407 Biología Celular 3104.11 Reproducción 2401.07 Embriología Animal |
| status_str | publishedVersion |
| title | Impact of sperm DNA damage and oocyte-repairing capacity on trout development |
| title_full | Impact of sperm DNA damage and oocyte-repairing capacity on trout development |
| title_fullStr | Impact of sperm DNA damage and oocyte-repairing capacity on trout development |
| title_full_unstemmed | Impact of sperm DNA damage and oocyte-repairing capacity on trout development |
| title_short | Impact of sperm DNA damage and oocyte-repairing capacity on trout development |
| title_sort | Impact of sperm DNA damage and oocyte-repairing capacity on trout development |
| topic | Biología Sperm DNA damage Oocyte repairing Trout development 2407 Biología Celular 3104.11 Reproducción 2401.07 Embriología Animal |
| topic_facet | Biología Sperm DNA damage Oocyte repairing Trout development 2407 Biología Celular 3104.11 Reproducción 2401.07 Embriología Animal |
| url | https://rep.bioscientifica.com/view/journals/rep/152/1/57.xml https://hdl.handle.net/10612/18707 |