T-TAs® 01 as a new tool for the evaluation of hemostasis in thrombocytopenic patients after platelet transfusion
background - Current laboratory tests fail to evaluate the hemostatic function of platelets in patients with thrombocytopenia. We investigated the use of the Total Thrombus-Formation Analysis System (T-TAS® 01 [Fujimori Kogyo Co, Tokyo, Japan]) to evaluate hemostasis under conditions of experimental...
| Authors: | ; ; ; ; ; ; ; ; ; |
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| Format: | Article |
| Publication date: | 2024 |
| Country: | España |
| Institution: | Universitat Autònoma de Barcelona |
| Repository: | Dipòsit Digital de Documents de la UAB |
| Language: | English |
| OAI Identifier: | oai:ddd.uab.cat:302112 |
| Online Access: | https://ddd.uab.cat/record/302112 https://dx.doi.org/urn:doi:10.2450/BloodTransfus.550 |
| Access Level: | Open access |
| Keyword: | Thrombocytopenia Total thrombus-formation analysis system (T-TAS) Platelet transfusion Bleeding |
| _version_ | 1878729685113765888 |
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| author | Sanz, Cristina |
| author2 | Escolar, Ginès Diaz-Ricart, Maribel|||0000-0003-1122-0052 Samanbar, Sahar Piñeyroa, Juan A. Moreno-Castaño, Ana Belén|||0000-0003-0338-7514 Pino, Marc Torramadé-Moix, Sergi Martínez Sánchez, Julia Lozano, Miquel|||0000-0003-2593-833X |
| author2_role | author author author author author author author author author |
| author_browse | Diaz-Ricart, Maribel|||0000-0003-1122-0052 Escolar, Ginès Lozano, Miquel|||0000-0003-2593-833X Martínez Sánchez, Julia Moreno-Castaño, Ana Belén|||0000-0003-0338-7514 Pino, Marc Piñeyroa, Juan A. Samanbar, Sahar Sanz, Cristina Torramadé-Moix, Sergi |
| author_facet | Sanz, Cristina Escolar, Ginès Diaz-Ricart, Maribel|||0000-0003-1122-0052 Samanbar, Sahar Piñeyroa, Juan A. Moreno-Castaño, Ana Belén|||0000-0003-0338-7514 Pino, Marc Torramadé-Moix, Sergi Martínez Sánchez, Julia Lozano, Miquel|||0000-0003-2593-833X |
| author_role | author |
| collection | Dipòsit Digital de Documents de la UAB |
| description | background - Current laboratory tests fail to evaluate the hemostatic function of platelets in patients with thrombocytopenia. We investigated the use of the Total Thrombus-Formation Analysis System (T-TAS® 01 [Fujimori Kogyo Co, Tokyo, Japan]) to evaluate hemostasis under conditions of experimental thrombocytopenia, and in patients before and after platelet transfusion. materials and methods - Specific T-TAS 01 chips, for thrombocytopenic conditions, were used. The area under the curve (AUC) and occlusion time (OT, min) were measured in: (i) experimentally induced thrombocytopenia (183±15 to 6.3±1.2×10 platelets/µL) in blood samples from healthy donors (No.=13), and (ii) blood from oncohematological thrombocytopenic patients (No.=48), before and after platelet transfusion. The influences of hematocrit and number of transfusions were analyzed in these patients. results - Progressive reductions of AUC and prolongations of OT related significantly to decreasing platelet counts (p<0.05 for all) in experimental thrombocytopenia. In samples from thrombocytopenic patients, platelet counts, AUC and OT were, respectively, 10.8±0.6×10/µL, 175.2±59, and 27.2±1 min before transfusion; and 22±1.5×10/µL, 400.8±83 and 22.9±1.5 min after platelet transfusion (p<0.01 for all). A hematocrit below 25% or exposure to ten or more previous platelet transfusions had a negative impact on the T-TAS 01 performance in patients. In vitro correction of the hematocrit improved the hemostatic response in thrombocytopenic patients. discussion - T-TAS 01 measurements were sensitive to low platelet counts in the experimental setting. The technology was sensitive to evaluate the hemostatic capacity of platelet transfusions. Exposure to multiple medications, repeated platelet transfusions and lower hematocrits may interfere with the hemostatic performance in oncohematological patients with thrombocytopenia. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_0cedc87c8571ec0cc5fbcd603ee77cd8 |
| institution | Universitat Autònoma de Barcelona |
| instname_str | Universitat Autònoma de Barcelona |
| language | eng |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:ddd.uab.cat:302112 |
| publishDate | 2024 |
| publishDateSort | 2024 |
| reponame_str | Dipòsit Digital de Documents de la UAB |
| spelling | T-TAs® 01 as a new tool for the evaluation of hemostasis in thrombocytopenic patients after platelet transfusion Sanz, Cristina Escolar, Ginès Diaz-Ricart, Maribel|||0000-0003-1122-0052 Samanbar, Sahar Piñeyroa, Juan A. Moreno-Castaño, Ana Belén|||0000-0003-0338-7514 Pino, Marc Torramadé-Moix, Sergi Martínez Sánchez, Julia Lozano, Miquel|||0000-0003-2593-833X Thrombocytopenia Total thrombus-formation analysis system (T-TAS) Platelet transfusion Bleeding background - Current laboratory tests fail to evaluate the hemostatic function of platelets in patients with thrombocytopenia. We investigated the use of the Total Thrombus-Formation Analysis System (T-TAS® 01 [Fujimori Kogyo Co, Tokyo, Japan]) to evaluate hemostasis under conditions of experimental thrombocytopenia, and in patients before and after platelet transfusion. materials and methods - Specific T-TAS 01 chips, for thrombocytopenic conditions, were used. The area under the curve (AUC) and occlusion time (OT, min) were measured in: (i) experimentally induced thrombocytopenia (183±15 to 6.3±1.2×10 platelets/µL) in blood samples from healthy donors (No.=13), and (ii) blood from oncohematological thrombocytopenic patients (No.=48), before and after platelet transfusion. The influences of hematocrit and number of transfusions were analyzed in these patients. results - Progressive reductions of AUC and prolongations of OT related significantly to decreasing platelet counts (p<0.05 for all) in experimental thrombocytopenia. In samples from thrombocytopenic patients, platelet counts, AUC and OT were, respectively, 10.8±0.6×10/µL, 175.2±59, and 27.2±1 min before transfusion; and 22±1.5×10/µL, 400.8±83 and 22.9±1.5 min after platelet transfusion (p<0.01 for all). A hematocrit below 25% or exposure to ten or more previous platelet transfusions had a negative impact on the T-TAS 01 performance in patients. In vitro correction of the hematocrit improved the hemostatic response in thrombocytopenic patients. discussion - T-TAS 01 measurements were sensitive to low platelet counts in the experimental setting. The technology was sensitive to evaluate the hemostatic capacity of platelet transfusions. Exposure to multiple medications, repeated platelet transfusions and lower hematocrits may interfere with the hemostatic performance in oncohematological patients with thrombocytopenia. https://ddd.uab.cat/record/302112 https://dx.doi.org/urn:doi:10.2450/BloodTransfus.550 |
| spellingShingle | T-TAs® 01 as a new tool for the evaluation of hemostasis in thrombocytopenic patients after platelet transfusion Sanz, Cristina Thrombocytopenia Total thrombus-formation analysis system (T-TAS) Platelet transfusion Bleeding |
| title | T-TAs® 01 as a new tool for the evaluation of hemostasis in thrombocytopenic patients after platelet transfusion |
| title_full | T-TAs® 01 as a new tool for the evaluation of hemostasis in thrombocytopenic patients after platelet transfusion |
| title_fullStr | T-TAs® 01 as a new tool for the evaluation of hemostasis in thrombocytopenic patients after platelet transfusion |
| title_full_unstemmed | T-TAs® 01 as a new tool for the evaluation of hemostasis in thrombocytopenic patients after platelet transfusion |
| title_short | T-TAs® 01 as a new tool for the evaluation of hemostasis in thrombocytopenic patients after platelet transfusion |
| title_sort | T-TAs® 01 as a new tool for the evaluation of hemostasis in thrombocytopenic patients after platelet transfusion |
| topic | Thrombocytopenia Total thrombus-formation analysis system (T-TAS) Platelet transfusion Bleeding |
| topic_facet | Thrombocytopenia Total thrombus-formation analysis system (T-TAS) Platelet transfusion Bleeding |
| url | https://ddd.uab.cat/record/302112 https://dx.doi.org/urn:doi:10.2450/BloodTransfus.550 |