Protein/polysaccharide complexes to stabilize decane-in-water nanoemulsions
Protein/polysaccharide complexes can be formed by electrostatic interactions and may be useful for enhancing the stability of nanoemulsions containing short-chain alkanes, which are highly prone to destabilization by Ostwald ripening. The study aimed to assess the capacity of biopolymer complexes co...
| Authors: | ; ; ; ; ; |
|---|---|
| Format: | Article |
| Status: | Accepted version |
| Publication date: | 2020 |
| Country: | España |
| Institution: | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| Repository: | Recercat. Dipósit de la Recerca de Catalunya |
| OAI Identifier: | oai:recercat.cat:10459.1/69395 |
| Online Access: | https://doi.org/10.1007/s11483-019-09622-x http://hdl.handle.net/10459.1/69395 |
| Access Level: | Open access |
| Keyword: | Whey protein Sugar beet pectin Protein/polysaccharide complexes Interfacial rheology |
| _version_ | 1878729681463672832 |
|---|---|
| author | Artiga Artigas, María |
| author2 | Reichert, Corina Salvia Trujillo, Laura Zeeb, Benjamin Martín Belloso, Olga Weiss, Jochen |
| author2_role | author author author author author |
| author_browse | Artiga Artigas, María Martín Belloso, Olga Reichert, Corina Salvia Trujillo, Laura Weiss, Jochen Zeeb, Benjamin |
| author_facet | Artiga Artigas, María Reichert, Corina Salvia Trujillo, Laura Zeeb, Benjamin Martín Belloso, Olga Weiss, Jochen |
| author_role | author |
| collection | Recercat. Dipósit de la Recerca de Catalunya |
| description | Protein/polysaccharide complexes can be formed by electrostatic interactions and may be useful for enhancing the stability of nanoemulsions containing short-chain alkanes, which are highly prone to destabilization by Ostwald ripening. The study aimed to assess the capacity of biopolymer complexes composed of whey protein isolate (WPI) and sugar beet pectin (SBP) to form and stabilize interfacially structured nanoemulsions. Nanoemulsions were stored for 21 days at room temperature to assess their stability against Ostwald ripening over time. Complexes showed higher emulsifying capacity than biopolymers alone since particle size of complex-stabilized nanoemulsions remained stable (d(4;3)similar to 0.26 mu m) for at least 48 h after preparation, whereas WPI- or SBP-stabilized nanoemulsions were prone to destabilization during the first 24 h reaching values around 1 mu m. Moreover, while the final particle size observed for the latter during the 21 days of storage was around 8 mu m, complex-stabilized nanoemulsions exhibited particle sizes up to 2.34 mu m, which had a direct impact in delaying creaming. Moreover, complex-stabilized nanoemulsions exhibited negative zeta-potential with similar values to those stabilized by SBP (-20.4 and - 22.1 mV, respectively) while the interfacial rheology behavior of complex-stabilized systems was more similar to those stabilized by WPI. This evidences that the protein fraction may be adsorbed at the oil interface thus dominating the interface rheology, whereas pectin chains located on the periphery of the complex and oriented towards the water phase may confer negative interfacial charge to oil droplets. These results indicated that WPI/SBP complexes were more effective than the biopolymers alone in preventing Ostwald ripening in decane-in-water nanoemulsions. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_0ccf49a85e9fe8a0cde331f62dd2b0aa |
| institution | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| instname_str | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:recercat.cat:10459.1/69395 |
| publishDate | 2020 |
| publishDateSort | 2020 |
| publisher | Springer Science+Business Media, LLC, part of Springer Nature |
| publisherStr | Springer Science+Business Media, LLC, part of Springer Nature |
| reponame_str | Recercat. Dipósit de la Recerca de Catalunya |
| spelling | Protein/polysaccharide complexes to stabilize decane-in-water nanoemulsions Artiga Artigas, María Reichert, Corina Salvia Trujillo, Laura Zeeb, Benjamin Martín Belloso, Olga Weiss, Jochen Whey protein Sugar beet pectin Protein/polysaccharide complexes Interfacial rheology Protein/polysaccharide complexes can be formed by electrostatic interactions and may be useful for enhancing the stability of nanoemulsions containing short-chain alkanes, which are highly prone to destabilization by Ostwald ripening. The study aimed to assess the capacity of biopolymer complexes composed of whey protein isolate (WPI) and sugar beet pectin (SBP) to form and stabilize interfacially structured nanoemulsions. Nanoemulsions were stored for 21 days at room temperature to assess their stability against Ostwald ripening over time. Complexes showed higher emulsifying capacity than biopolymers alone since particle size of complex-stabilized nanoemulsions remained stable (d(4;3)similar to 0.26 mu m) for at least 48 h after preparation, whereas WPI- or SBP-stabilized nanoemulsions were prone to destabilization during the first 24 h reaching values around 1 mu m. Moreover, while the final particle size observed for the latter during the 21 days of storage was around 8 mu m, complex-stabilized nanoemulsions exhibited particle sizes up to 2.34 mu m, which had a direct impact in delaying creaming. Moreover, complex-stabilized nanoemulsions exhibited negative zeta-potential with similar values to those stabilized by SBP (-20.4 and - 22.1 mV, respectively) while the interfacial rheology behavior of complex-stabilized systems was more similar to those stabilized by WPI. This evidences that the protein fraction may be adsorbed at the oil interface thus dominating the interface rheology, whereas pectin chains located on the periphery of the complex and oriented towards the water phase may confer negative interfacial charge to oil droplets. These results indicated that WPI/SBP complexes were more effective than the biopolymers alone in preventing Ostwald ripening in decane-in-water nanoemulsions. This study was funded by the Ministry of Economy, Industry and Competitiveness (MINECO/FEDER, UE) throughout project AGL2015-65975-R. Author María Artiga-Artigas thanks the University of Lleida for their pre-doctoral fellowship. Author Laura Salvia-Trujillo thanks the “Secretaria d’Universitats i Recerca del Departament d’Empresa i Coneixement de la Generalitat de Catalunya” for the Beatriu de Pinós post-doctoral grant BdP2016 00336. Springer Science+Business Media, LLC, part of Springer Nature https://doi.org/10.1007/s11483-019-09622-x http://hdl.handle.net/10459.1/69395 |
| spellingShingle | Protein/polysaccharide complexes to stabilize decane-in-water nanoemulsions Artiga Artigas, María Whey protein Sugar beet pectin Protein/polysaccharide complexes Interfacial rheology |
| status_str | acceptedVersion |
| title | Protein/polysaccharide complexes to stabilize decane-in-water nanoemulsions |
| title_full | Protein/polysaccharide complexes to stabilize decane-in-water nanoemulsions |
| title_fullStr | Protein/polysaccharide complexes to stabilize decane-in-water nanoemulsions |
| title_full_unstemmed | Protein/polysaccharide complexes to stabilize decane-in-water nanoemulsions |
| title_short | Protein/polysaccharide complexes to stabilize decane-in-water nanoemulsions |
| title_sort | Protein/polysaccharide complexes to stabilize decane-in-water nanoemulsions |
| topic | Whey protein Sugar beet pectin Protein/polysaccharide complexes Interfacial rheology |
| topic_facet | Whey protein Sugar beet pectin Protein/polysaccharide complexes Interfacial rheology |
| url | https://doi.org/10.1007/s11483-019-09622-x http://hdl.handle.net/10459.1/69395 |