The Use of P63 Immunohistochemistry for the Identification of Squamous Cell Carcinoma of the Lung
Introduction: While some targeted agents should not be used in squamous cell carcinomas (SCCs), other agents might preferably target SCCs. In a previous microarray study, one of the top differentially expressed genes between adenocarcinomas (ACs) and SCCs is P63. It is a well-known marker of squamou...
| Autores: | ; ; ; ; ; ; ; ; ; ; ; ; |
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| Tipo de documento: | Artículo |
| Estado: | Published version |
| Fecha de publicación: | 2010 |
| País: | España |
| Institución: | Universidad de Barcelona |
| Repositorio: | Dipòsit Digital de la UB |
| OAI Identifier: | oai:diposit.ub.edu:2445/126804 |
| Acceso en línea: | https://hdl.handle.net/2445/126804 |
| Nivel de acceso: | Acceso abierto |
| Palabra clave: | Càncer de pulmó Immunohistoquímica Lung cancer Immunohistochemistry |
| _version_ | 1878729437315334144 |
|---|---|
| author | Conde, Esther |
| author2 | Angulo, Bárbara Redondo, Pilar Toldos, Oscar García García, Elena Suárez Gauthier, Ana Rubio Viqueira, Belén Marrón, Carmen García Luján, Ricardo Sánchez Céspedes, Montserrat López Encuentra, Agel Paz-Ares, Luis López Ríos, Fernando |
| author2_role | author author author author author author author author author author author author |
| author_browse | Angulo, Bárbara Conde, Esther García García, Elena García Luján, Ricardo López Encuentra, Agel López Ríos, Fernando Marrón, Carmen Paz-Ares, Luis Redondo, Pilar Rubio Viqueira, Belén Suárez Gauthier, Ana Sánchez Céspedes, Montserrat Toldos, Oscar |
| author_facet | Conde, Esther Angulo, Bárbara Redondo, Pilar Toldos, Oscar García García, Elena Suárez Gauthier, Ana Rubio Viqueira, Belén Marrón, Carmen García Luján, Ricardo Sánchez Céspedes, Montserrat López Encuentra, Agel Paz-Ares, Luis López Ríos, Fernando |
| author_role | author |
| collection | Dipòsit Digital de la UB |
| description | Introduction: While some targeted agents should not be used in squamous cell carcinomas (SCCs), other agents might preferably target SCCs. In a previous microarray study, one of the top differentially expressed genes between adenocarcinomas (ACs) and SCCs is P63. It is a well-known marker of squamous differentiation, but surprisingly, its expression is not widely used for this purpose. Our goals in this study were (1) to further confirm our microarray data, (2) to analize the value of P63 immunohistochemistry (IHC) in reducing the number of large cell carcinoma (LCC) diagnoses in surgical specimens, and (3) to investigate the potential of P63 IHC to minimize the proportion of "carcinoma NOS (not otherwise specified)'' in a prospective series of small tumor samples. Methods: With these goals in mind, we studied (1) a tissue-microarray comprising 33 ACs and 99 SCCs on which we performed P63 IHC, (2) a series of 20 surgically resected LCCs studied for P63 and TTF-1 IHC, and (3) a prospective cohort of 66 small thoracic samples, including 32 carcinoma NOS, that were further classified by the result of P63 and TTF-1 IHC. Results: The results in the three independent cohorts were as follows: (1) P63 IHC was differentially expressed in SCCs when compared to ACs (p<0.0001); (2) half of the 20 (50%) LCCs were positive for P63 and were reclassified as SCCs; and (3) all P63 positive cases (34%) were diagnosed as SCCs. Conclusions: P63 IHC is useful for the identification of lung SCCs. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_07e81a0d3fde3ca7e7449946a8304e15 |
| institution | Universidad de Barcelona |
| instname_str | Universidad de Barcelona |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:diposit.ub.edu:2445/126804 |
| publishDate | 2010 |
| publishDateSort | 2010 |
| publisher | Public Library of Science (PLoS) |
| publisherStr | Public Library of Science (PLoS) |
| reponame_str | Dipòsit Digital de la UB |
| spelling | The Use of P63 Immunohistochemistry for the Identification of Squamous Cell Carcinoma of the Lung Conde, Esther Angulo, Bárbara Redondo, Pilar Toldos, Oscar García García, Elena Suárez Gauthier, Ana Rubio Viqueira, Belén Marrón, Carmen García Luján, Ricardo Sánchez Céspedes, Montserrat López Encuentra, Agel Paz-Ares, Luis López Ríos, Fernando Càncer de pulmó Immunohistoquímica Lung cancer Immunohistochemistry Introduction: While some targeted agents should not be used in squamous cell carcinomas (SCCs), other agents might preferably target SCCs. In a previous microarray study, one of the top differentially expressed genes between adenocarcinomas (ACs) and SCCs is P63. It is a well-known marker of squamous differentiation, but surprisingly, its expression is not widely used for this purpose. Our goals in this study were (1) to further confirm our microarray data, (2) to analize the value of P63 immunohistochemistry (IHC) in reducing the number of large cell carcinoma (LCC) diagnoses in surgical specimens, and (3) to investigate the potential of P63 IHC to minimize the proportion of "carcinoma NOS (not otherwise specified)'' in a prospective series of small tumor samples. Methods: With these goals in mind, we studied (1) a tissue-microarray comprising 33 ACs and 99 SCCs on which we performed P63 IHC, (2) a series of 20 surgically resected LCCs studied for P63 and TTF-1 IHC, and (3) a prospective cohort of 66 small thoracic samples, including 32 carcinoma NOS, that were further classified by the result of P63 and TTF-1 IHC. Results: The results in the three independent cohorts were as follows: (1) P63 IHC was differentially expressed in SCCs when compared to ACs (p<0.0001); (2) half of the 20 (50%) LCCs were positive for P63 and were reclassified as SCCs; and (3) all P63 positive cases (34%) were diagnosed as SCCs. Conclusions: P63 IHC is useful for the identification of lung SCCs. Public Library of Science (PLoS) https://hdl.handle.net/2445/126804 |
| spellingShingle | The Use of P63 Immunohistochemistry for the Identification of Squamous Cell Carcinoma of the Lung Conde, Esther Càncer de pulmó Immunohistoquímica Lung cancer Immunohistochemistry |
| status_str | publishedVersion |
| title | The Use of P63 Immunohistochemistry for the Identification of Squamous Cell Carcinoma of the Lung |
| title_full | The Use of P63 Immunohistochemistry for the Identification of Squamous Cell Carcinoma of the Lung |
| title_fullStr | The Use of P63 Immunohistochemistry for the Identification of Squamous Cell Carcinoma of the Lung |
| title_full_unstemmed | The Use of P63 Immunohistochemistry for the Identification of Squamous Cell Carcinoma of the Lung |
| title_short | The Use of P63 Immunohistochemistry for the Identification of Squamous Cell Carcinoma of the Lung |
| title_sort | The Use of P63 Immunohistochemistry for the Identification of Squamous Cell Carcinoma of the Lung |
| topic | Càncer de pulmó Immunohistoquímica Lung cancer Immunohistochemistry |
| topic_facet | Càncer de pulmó Immunohistoquímica Lung cancer Immunohistochemistry |
| url | https://hdl.handle.net/2445/126804 |