Storage conditions and stability of global DNA methylation in placental tissue
AIM: The placenta is an informative and easily available tissue for many epidemiological studies. We analyzed the extent to which storage delay affects DNA methylation. MATERIAL & METHODS: Biopsies from two placentas were sequentially stored at -80°C after standing at room temperature for 30 min...
| Authors: | ; ; ; ; ; ; ; |
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| Format: | Article |
| Status: | Accepted version |
| Publication date: | 2013 |
| Country: | España |
| Institution: | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| Repository: | Recercat. Dipósit de la Recerca de Catalunya |
| OAI Identifier: | oai:recercat.cat:10230/36592 |
| Online Access: | http://hdl.handle.net/10230/36592 http://dx.doi.org/10.2217/epi.13.29 |
| Access Level: | Open access |
| Keyword: | ADN -- Metabolisme ADN -- Metilació Placenta -- Metabolisme |
| _version_ | 1878729365304377344 |
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| author | Vilahur Chiaraviglio, Nadia, 1982- |
| author2 | Baccarelli, Andrea A. Bustamante Pineda, Mariona Agramunt, Silvia Byun, Hyang-Min Fernandez, Mariana F. Sunyer Deu, Jordi Estivill, Xavier, 1955- |
| author2_role | author author author author author author author |
| author_browse | Agramunt, Silvia Baccarelli, Andrea A. Bustamante Pineda, Mariona Byun, Hyang-Min Estivill, Xavier, 1955- Fernandez, Mariana F. Sunyer Deu, Jordi Vilahur Chiaraviglio, Nadia, 1982- |
| author_facet | Vilahur Chiaraviglio, Nadia, 1982- Baccarelli, Andrea A. Bustamante Pineda, Mariona Agramunt, Silvia Byun, Hyang-Min Fernandez, Mariana F. Sunyer Deu, Jordi Estivill, Xavier, 1955- |
| author_role | author |
| collection | Recercat. Dipósit de la Recerca de Catalunya |
| description | AIM: The placenta is an informative and easily available tissue for many epidemiological studies. We analyzed the extent to which storage delay affects DNA methylation. MATERIAL & METHODS: Biopsies from two placentas were sequentially stored at -80°C after standing at room temperature for 30 min, 1 h, 2 h, 6 h and 24 h. Global DNA methylation was measured by bisulfite pyrosequencing of repetitive elements and the luminometric methylation assay. RESULTS: Small changes in global DNA methylation in relation to time-to-storage were observed by pyrosequencing, with a coefficient of variation (COV) of 2.49% (placenta 1) and 2.86% (placenta 2), similar to the mean technical variation observed for pyrosequencing (COV: 1.91 and 1.51%, respectively). A luminometric methylation assay yielded more variable results in the two placentas analyzed, both among time points (COV: 9.13 and 10.35%, respectively) and technical replicates (COV: 11.60 and 9.80%, respectively). CONCLUSION: Global DNA methylation is stable at room temperature. However, some techniques to measure methylation might be confounded by DNA degradation caused by a delay in storage. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_0683fc83e08cffebb2ce76c462e2eb9d |
| institution | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| instname_str | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:recercat.cat:10230/36592 |
| publishDate | 2013 |
| publishDateSort | 2013 |
| publisher | Future Medicine |
| publisherStr | Future Medicine |
| reponame_str | Recercat. Dipósit de la Recerca de Catalunya |
| spelling | Storage conditions and stability of global DNA methylation in placental tissue Vilahur Chiaraviglio, Nadia, 1982- Baccarelli, Andrea A. Bustamante Pineda, Mariona Agramunt, Silvia Byun, Hyang-Min Fernandez, Mariana F. Sunyer Deu, Jordi Estivill, Xavier, 1955- ADN -- Metabolisme ADN -- Metilació Placenta -- Metabolisme AIM: The placenta is an informative and easily available tissue for many epidemiological studies. We analyzed the extent to which storage delay affects DNA methylation. MATERIAL & METHODS: Biopsies from two placentas were sequentially stored at -80°C after standing at room temperature for 30 min, 1 h, 2 h, 6 h and 24 h. Global DNA methylation was measured by bisulfite pyrosequencing of repetitive elements and the luminometric methylation assay. RESULTS: Small changes in global DNA methylation in relation to time-to-storage were observed by pyrosequencing, with a coefficient of variation (COV) of 2.49% (placenta 1) and 2.86% (placenta 2), similar to the mean technical variation observed for pyrosequencing (COV: 1.91 and 1.51%, respectively). A luminometric methylation assay yielded more variable results in the two placentas analyzed, both among time points (COV: 9.13 and 10.35%, respectively) and technical replicates (COV: 11.60 and 9.80%, respectively). CONCLUSION: Global DNA methylation is stable at room temperature. However, some techniques to measure methylation might be confounded by DNA degradation caused by a delay in storage. This study was funded by grants from the Spanish Ministry of Health (FIS-PI041436 and PI11/00610), Instituto de Salud Carlos III (Red Infancia y MedioAmbiente G03/176 and CB06/02/0041) and the Generalitat de Catalunya-Comissió Interdepartamental de Recerca i Innovació Tecnològica 1999SGR 00241. AA Baccarelli receives support from the Harvard School of Public Health and National Institute of Environmental Health Sciences Center for Environmental Health (ES000002). N Vilahur was supported by a Formación de Personal Investigador Grant from the Spanish Ministry of Health and a Formación de Personal Investigador Grant for Short Research Stays in Foreign Institutions (BES-2009-023933) and MF Fernandez by the Ramon y Cajal Research Grant from the Spanish Ministry of Education Future Medicine http://hdl.handle.net/10230/36592 http://dx.doi.org/10.2217/epi.13.29 |
| spellingShingle | Storage conditions and stability of global DNA methylation in placental tissue Vilahur Chiaraviglio, Nadia, 1982- ADN -- Metabolisme ADN -- Metilació Placenta -- Metabolisme |
| status_str | acceptedVersion |
| title | Storage conditions and stability of global DNA methylation in placental tissue |
| title_full | Storage conditions and stability of global DNA methylation in placental tissue |
| title_fullStr | Storage conditions and stability of global DNA methylation in placental tissue |
| title_full_unstemmed | Storage conditions and stability of global DNA methylation in placental tissue |
| title_short | Storage conditions and stability of global DNA methylation in placental tissue |
| title_sort | Storage conditions and stability of global DNA methylation in placental tissue |
| topic | ADN -- Metabolisme ADN -- Metilació Placenta -- Metabolisme |
| topic_facet | ADN -- Metabolisme ADN -- Metilació Placenta -- Metabolisme |
| url | http://hdl.handle.net/10230/36592 http://dx.doi.org/10.2217/epi.13.29 |