Metataxomics reveals Blastocystis subtypes mixed infections in Colombian children

ABSTRACT: Blastocystis sp., is an intestinal protist with a broad host range and a high prevalence in human populations worldwide, even in developed Western countries. The publication of conflicting evidence has divided the scientific community about the pathogenic role of this parasite. Even though...

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Autores: García Montoya, Gisela María; Galván Díaz, Ana Luz; Alzate Restrepo, Juan Fernando
Tipo de documento: Artigo
Estado:Published version
Data de publicação:2023
País:Colombia
Recursos:Universidad de Antioquia
Repositório:Repositorio UdeA
Idioma:Inglês
OAI Identifier:oai:bibliotecadigital.udea.edu.co:10495/43461
Acesso em linha:https://hdl.handle.net/10495/43461
Nível de acesso:Acesso aberto
Palavra-chave:Infecciones por Blastocystis
Blastocystis Infections
Blastocystis - genética
Blastocystis - genetics
Colombia - epidemiología
Colombia - epidemiology
Niño
Child
Coinfección
Coinfection
ADN Protozoario
DNA, Protozoan
Heces - parasitología
Feces / parasitology
Variación Genética
Genetic Variation
Filogenia
Phylogeny
https://id.nlm.nih.gov/mesh/D016776
https://id.nlm.nih.gov/mesh/D016844
https://id.nlm.nih.gov/mesh/D003105
https://id.nlm.nih.gov/mesh/D002648
https://id.nlm.nih.gov/mesh/D060085
https://id.nlm.nih.gov/mesh/D016054
https://id.nlm.nih.gov/mesh/D005243
https://id.nlm.nih.gov/mesh/D014644
https://id.nlm.nih.gov/mesh/D010802
Resumo:ABSTRACT: Blastocystis sp., is an intestinal protist with a broad host range and a high prevalence in human populations worldwide, even in developed Western countries. The publication of conflicting evidence has divided the scientific community about the pathogenic role of this parasite. Even though, genetic studies on Blastocystis sp. revealed associations between genotypes and different pathogenic profiles. Conventionally, the detection of this parasite is based on microscopic or PCR methods, which offer meager or null performance in detecting mixed infections. In this work, we applied a metataxonomic NGS approach targeting the V4 region of the eukaryotic SSU-rRNA gene and classical phylogenetic methods. This approach allowed us to detect Blastocystis sp. in stool samples from infected children living in an urban setting in the city of Medellin attending the same daycare center. Phylogenetic analysis identified the subtypes present in the children as ST1, ST2, and ST3. Besides, mixed infections of subtypes ST1 + ST3 were spotted in 16% of the analyzed stool samples.

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