Differentially expressed proteins in positive versus negative HNSCC lymph nodes
Background: Lymph node metastasis is one of the most important prognostic factors in head and neck squamous cell carcinomas (HNSCCs) and critical for delineating their treatment. However, clinical and histological criteria for the diagnosis of nodal status remain limited. In the present study, we ai...
| Authors: | ; ; ; ; ; ; ; ; ; ; ; |
|---|---|
| Format: | Article |
| Status: | Published version |
| Publication date: | 2018 |
| Country: | Brasil |
| Institution: | UNESP |
| Repository: | Repositório Institucional da UNESP |
| Language: | English |
| OAI Identifier: | oai:repositorio.unesp.br:11449/176786 |
| Online Access: | http://dx.doi.org/10.1186/s12920-018-0382-6 http://hdl.handle.net/11449/176786 |
| Access Level: | Open access |
| Keyword: | Head and neck carcinoma Lymph node Metastasis Proteomics |
| _version_ | 1878766869447442432 |
|---|---|
| author | Vidotto, Alessandra |
| author2 | Polachini, Giovana M. De Paula-Silva, Marina [UNESP] Oliani, Sonia M. [UNESP] Henrique, Tiago López, Rossana V. M. Cury, Patrícia M. Nunes, Fabio D. Góis-Filho, José F. De Carvalho, Marcos B. Leopoldino, Andréia M. Tajara, Eloiza H. |
| author2_role | author author author author author author author author author author author |
| author_browse | Cury, Patrícia M. De Carvalho, Marcos B. De Paula-Silva, Marina [UNESP] Góis-Filho, José F. Henrique, Tiago Leopoldino, Andréia M. López, Rossana V. M. Nunes, Fabio D. Oliani, Sonia M. [UNESP] Polachini, Giovana M. Tajara, Eloiza H. Vidotto, Alessandra |
| author_facet | Vidotto, Alessandra Polachini, Giovana M. De Paula-Silva, Marina [UNESP] Oliani, Sonia M. [UNESP] Henrique, Tiago López, Rossana V. M. Cury, Patrícia M. Nunes, Fabio D. Góis-Filho, José F. De Carvalho, Marcos B. Leopoldino, Andréia M. Tajara, Eloiza H. |
| author_role | author |
| collection | Repositório Institucional da UNESP |
| description | Background: Lymph node metastasis is one of the most important prognostic factors in head and neck squamous cell carcinomas (HNSCCs) and critical for delineating their treatment. However, clinical and histological criteria for the diagnosis of nodal status remain limited. In the present study, we aimed to characterize the proteomic profile of lymph node metastasis from HNSCC patients. Methods: In the present study, we used one- and two-dimensional electrophoresis and mass spectrometry analysis to characterize the proteomic profile of lymph node metastasis from HNSCC. Results: Comparison of metastatic and non-metastatic lymph nodes showed 52 differentially expressed proteins associated with neoplastic development and progression. The results reinforced the idea that tumors from different anatomical subsites have dissimilar behaviors, which may be influenced by micro-environmental factor including the lymphatic network. The expression pattern of heat shock proteins and glycolytic enzymes also suggested an effect of the lymph node environment in controlling tumor growth or in metabolic reprogramming of the metastatic cell. Our study, for the first time, provided direct evidence of annexin A1 overexpression in lymph node metastasis of head and neck cancer, adding information that may be useful for diagnosing aggressive disease. Conclusions: In brief, this study contributed to our understanding of the metastatic phenotype of HNSCC and provided potential targets for diagnostic in this group of carcinomas. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | BR_eb8ea0dfd2d828d2e10d00905fda387f |
| institution | UNESP |
| instname_str | Universidade Estadual Paulista (UNESP) |
| language | eng |
| network_acronym_str | BR |
| network_name_str | Brasil |
| oai_identifier_str | oai:repositorio.unesp.br:11449/176786 |
| publishDate | 2018 |
| publishDateSort | 2018 |
| reponame_str | Repositório Institucional da UNESP |
| spelling | Differentially expressed proteins in positive versus negative HNSCC lymph nodes Vidotto, Alessandra Polachini, Giovana M. De Paula-Silva, Marina [UNESP] Oliani, Sonia M. [UNESP] Henrique, Tiago López, Rossana V. M. Cury, Patrícia M. Nunes, Fabio D. Góis-Filho, José F. De Carvalho, Marcos B. Leopoldino, Andréia M. Tajara, Eloiza H. Head and neck carcinoma Lymph node Metastasis Proteomics Background: Lymph node metastasis is one of the most important prognostic factors in head and neck squamous cell carcinomas (HNSCCs) and critical for delineating their treatment. However, clinical and histological criteria for the diagnosis of nodal status remain limited. In the present study, we aimed to characterize the proteomic profile of lymph node metastasis from HNSCC patients. Methods: In the present study, we used one- and two-dimensional electrophoresis and mass spectrometry analysis to characterize the proteomic profile of lymph node metastasis from HNSCC. Results: Comparison of metastatic and non-metastatic lymph nodes showed 52 differentially expressed proteins associated with neoplastic development and progression. The results reinforced the idea that tumors from different anatomical subsites have dissimilar behaviors, which may be influenced by micro-environmental factor including the lymphatic network. The expression pattern of heat shock proteins and glycolytic enzymes also suggested an effect of the lymph node environment in controlling tumor growth or in metabolic reprogramming of the metastatic cell. Our study, for the first time, provided direct evidence of annexin A1 overexpression in lymph node metastasis of head and neck cancer, adding information that may be useful for diagnosing aggressive disease. Conclusions: In brief, this study contributed to our understanding of the metastatic phenotype of HNSCC and provided potential targets for diagnostic in this group of carcinomas. Departamento de Biologia Molecular Faculdade de Medicina (FAMERP), Av. Brigadeiro Faria Lima, 5416, Vila São Pedro Departamento de Biologia Instituto de Biociências Letras e Ciências Exatas (IBILCE) Universidade Estadual Paulista (UNESP), R. Cristóvão Colombo, 2265 Instituto Do Câncer de São Paulo Octavio Frias de Oliveira - ICESP, Av. Dr. Arnaldo, 251 - Cerqueira César Faculdade Ceres (Faceres), Av. Anísio Haddad, 6751 Departamento de Estomatologia Faculdade de Odontologia Universidade de São Paulo, Av. Prof. Lineu Prestes, 2227 Instituto Do Câncer Arnaldo Vieira de Carvalho, R. Dr Cesário Mota Jr, 112 Departamento de Cirurgia de Cabeça e Pescoço Hospital Heliópolis, R. Cônego Xavier, 276 Departamento de Análises Clínicas Toxicológicas e Bromatológicas Faculdade de Ciências Farmacêuticas Universidade de São Paulo, Avenida do Café, s/n Departamento de Genética e Biologia Evolutiva Instituto de Biociências Universidade de São Paulo, R. do Matão, 321 http://dx.doi.org/10.1186/s12920-018-0382-6 BMC Medical Genomics, v. 11, n. 1, 2018. 1755-8794 http://hdl.handle.net/11449/176786 10.1186/s12920-018-0382-6 2-s2.0-85052745406 2-s2.0-85052745406.pdf |
| spellingShingle | Differentially expressed proteins in positive versus negative HNSCC lymph nodes Vidotto, Alessandra Head and neck carcinoma Lymph node Metastasis Proteomics |
| status_str | publishedVersion |
| title | Differentially expressed proteins in positive versus negative HNSCC lymph nodes |
| title_full | Differentially expressed proteins in positive versus negative HNSCC lymph nodes |
| title_fullStr | Differentially expressed proteins in positive versus negative HNSCC lymph nodes |
| title_full_unstemmed | Differentially expressed proteins in positive versus negative HNSCC lymph nodes |
| title_short | Differentially expressed proteins in positive versus negative HNSCC lymph nodes |
| title_sort | Differentially expressed proteins in positive versus negative HNSCC lymph nodes |
| topic | Head and neck carcinoma Lymph node Metastasis Proteomics |
| topic_facet | Head and neck carcinoma Lymph node Metastasis Proteomics |
| url | http://dx.doi.org/10.1186/s12920-018-0382-6 http://hdl.handle.net/11449/176786 |