HPLC-FLD methods to quantify chloroaluminum phthalocyanine in nanoparticles, plasma and tissue : application in pharmacokinetic and biodistribution studies.
Analytical and bioanalytical methods of high-performance liquid chromatography with fluorescence detection (HPLC-FLD) were developed and validated for the determination of chloroaluminum phthalocyanine in different formulations of polymeric nanocapsules, plasma and livers of mice. Plasma and homogen...
| Authors: | ; ; ; ; |
|---|---|
| Format: | Article |
| Status: | Published version |
| Publication date: | 2011 |
| Country: | Brasil |
| Institution: | UFOP |
| Repository: | Repositório Institucional da UFOP |
| Language: | English |
| OAI Identifier: | oai:repositorio.ufop.br:123456789/3770 |
| Online Access: | http://www.repositorio.ufop.br/handle/123456789/3770 https://doi.org/10.1016/j.jpba.2011.04.016 |
| Access Level: | Open access |
| Keyword: | Chloroaluminium phthalocyanine High-performance liquid Nanocapsules Photodynamic therapy Fluorescent dye |
| _version_ | 1878766172167471104 |
|---|---|
| author | Oliveira, Liliam Teixeira |
| author2 | Garcia, Giani Martins Kano, Eunice Kazue Tedesco, Antônio Cláudio Mosqueira, Vanessa Carla Furtado |
| author2_role | author author author author |
| author_browse | Garcia, Giani Martins Kano, Eunice Kazue Mosqueira, Vanessa Carla Furtado Oliveira, Liliam Teixeira Tedesco, Antônio Cláudio |
| author_facet | Oliveira, Liliam Teixeira Garcia, Giani Martins Kano, Eunice Kazue Tedesco, Antônio Cláudio Mosqueira, Vanessa Carla Furtado |
| author_role | author |
| collection | Repositório Institucional da UFOP |
| description | Analytical and bioanalytical methods of high-performance liquid chromatography with fluorescence detection (HPLC-FLD) were developed and validated for the determination of chloroaluminum phthalocyanine in different formulations of polymeric nanocapsules, plasma and livers of mice. Plasma and homogenized liver samples were extracted with ethyl acetate, and zinc phthalocyanine was used as internal standard. The results indicated that the methods were linear and selective for all matrices studied. Analysis of accuracy and precision showed adequate values, with variations lower than 10% in biological samples and lower than 2% in analytical samples. The recoveries were as high as 96% and 99% in the plasma and livers, respectively. The quantification limit of the analytical method was 1.12 ng/ml, and the limits of quantification of the bioanalytical method were 15 ng/ml and 75 ng/g for plasma and liver samples, respectively. The bioanalytical method developed was sensitive in the ranges of 15–100 ng/ml in plasma and 75–500 ng/g in liver samples and was applied to studies of biodistribution and pharmacokinetics of AlClPc. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | BR_e1fa1da87e1bc2a9f4e4b245ae8ad4e2 |
| identifier_dark_str | ark:/61566/0013000003ckn |
| institution | UFOP |
| instname_str | Universidade Federal de Ouro Preto (UFOP) |
| language | eng |
| network_acronym_str | BR |
| network_name_str | Brasil |
| oai_identifier_str | oai:repositorio.ufop.br:123456789/3770 |
| publishDate | 2011 |
| publishDateSort | 2011 |
| reponame_str | Repositório Institucional da UFOP |
| spelling | HPLC-FLD methods to quantify chloroaluminum phthalocyanine in nanoparticles, plasma and tissue : application in pharmacokinetic and biodistribution studies. Oliveira, Liliam Teixeira Garcia, Giani Martins Kano, Eunice Kazue Tedesco, Antônio Cláudio Mosqueira, Vanessa Carla Furtado Chloroaluminium phthalocyanine High-performance liquid Nanocapsules Photodynamic therapy Fluorescent dye Analytical and bioanalytical methods of high-performance liquid chromatography with fluorescence detection (HPLC-FLD) were developed and validated for the determination of chloroaluminum phthalocyanine in different formulations of polymeric nanocapsules, plasma and livers of mice. Plasma and homogenized liver samples were extracted with ethyl acetate, and zinc phthalocyanine was used as internal standard. The results indicated that the methods were linear and selective for all matrices studied. Analysis of accuracy and precision showed adequate values, with variations lower than 10% in biological samples and lower than 2% in analytical samples. The recoveries were as high as 96% and 99% in the plasma and livers, respectively. The quantification limit of the analytical method was 1.12 ng/ml, and the limits of quantification of the bioanalytical method were 15 ng/ml and 75 ng/g for plasma and liver samples, respectively. The bioanalytical method developed was sensitive in the ranges of 15–100 ng/ml in plasma and 75–500 ng/g in liver samples and was applied to studies of biodistribution and pharmacokinetics of AlClPc. OLIVEIRA, L. M. et al. HPLC-FLD methods to quantify chloroaluminum phthalocyanine in nanoparticles, plasma and tissue : application in pharmacokinetic and biodistribution studies. Journal of Pharmaceutical and Biomedical Analysis. V. 56, p. 70-77, 2011. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0731708511002275>. Acesso em: 20 ago. 2014. 0731-7085 http://www.repositorio.ufop.br/handle/123456789/3770 https://doi.org/10.1016/j.jpba.2011.04.016 ark:/61566/0013000003ckn |
| spellingShingle | HPLC-FLD methods to quantify chloroaluminum phthalocyanine in nanoparticles, plasma and tissue : application in pharmacokinetic and biodistribution studies. Oliveira, Liliam Teixeira Chloroaluminium phthalocyanine High-performance liquid Nanocapsules Photodynamic therapy Fluorescent dye |
| status_str | publishedVersion |
| title | HPLC-FLD methods to quantify chloroaluminum phthalocyanine in nanoparticles, plasma and tissue : application in pharmacokinetic and biodistribution studies. |
| title_full | HPLC-FLD methods to quantify chloroaluminum phthalocyanine in nanoparticles, plasma and tissue : application in pharmacokinetic and biodistribution studies. |
| title_fullStr | HPLC-FLD methods to quantify chloroaluminum phthalocyanine in nanoparticles, plasma and tissue : application in pharmacokinetic and biodistribution studies. |
| title_full_unstemmed | HPLC-FLD methods to quantify chloroaluminum phthalocyanine in nanoparticles, plasma and tissue : application in pharmacokinetic and biodistribution studies. |
| title_short | HPLC-FLD methods to quantify chloroaluminum phthalocyanine in nanoparticles, plasma and tissue : application in pharmacokinetic and biodistribution studies. |
| title_sort | HPLC-FLD methods to quantify chloroaluminum phthalocyanine in nanoparticles, plasma and tissue : application in pharmacokinetic and biodistribution studies. |
| topic | Chloroaluminium phthalocyanine High-performance liquid Nanocapsules Photodynamic therapy Fluorescent dye |
| topic_facet | Chloroaluminium phthalocyanine High-performance liquid Nanocapsules Photodynamic therapy Fluorescent dye |
| url | http://www.repositorio.ufop.br/handle/123456789/3770 https://doi.org/10.1016/j.jpba.2011.04.016 |