The antileishmanial activity of the antarctic brown alga Ascoseira mirabilis Skottsberg
Leishmaniasis is a group of diseases that have limited and high toxic therapeutic options. Herein, we evaluated the antileishmanial potential and cytotoxicity of hexanic extract obtained from the Antarctic brown alga Ascoseira mirabilis using bioguided fractionation against Leishmania amazonensis an...
| Autores: | ; ; ; ; ; ; ; |
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| Tipo de documento: | Artículo |
| Estado: | Published version |
| Fecha de publicación: | 2020 |
| País: | Brasil |
| Institución: | UNESP |
| Repositorio: | Repositório Institucional da UNESP |
| Lenguaje: | Inglés |
| OAI Identifier: | oai:repositorio.unesp.br:11449/199062 |
| Acceso en línea: | http://dx.doi.org/10.1080/14786419.2020.1782403 http://hdl.handle.net/11449/199062 |
| Nivel de acceso: | Acceso abierto |
| Palabra clave: | Antarctic macroalgae Ascoseiraceae Brown algae Cutaneous leishmaniasis GC-MS Natural products |
| _version_ | 1878765976075370496 |
|---|---|
| author | Clementino, Leandro da Costa [UNESP] |
| author2 | Oda, Fernando Bombarda [UNESP] Teixeira, Thaiz Rodrigues Tavares, Renata Spagolla Napoleão Colepicolo, Pio Santos, André Gonzaga dos [UNESP] Debonsi, Hosana Maria Graminha, Márcia A. S. [UNESP] |
| author2_role | author author author author author author author |
| author_browse | Clementino, Leandro da Costa [UNESP] Colepicolo, Pio Debonsi, Hosana Maria Graminha, Márcia A. S. [UNESP] Oda, Fernando Bombarda [UNESP] Santos, André Gonzaga dos [UNESP] Tavares, Renata Spagolla Napoleão Teixeira, Thaiz Rodrigues |
| author_facet | Clementino, Leandro da Costa [UNESP] Oda, Fernando Bombarda [UNESP] Teixeira, Thaiz Rodrigues Tavares, Renata Spagolla Napoleão Colepicolo, Pio Santos, André Gonzaga dos [UNESP] Debonsi, Hosana Maria Graminha, Márcia A. S. [UNESP] |
| author_role | author |
| collection | Repositório Institucional da UNESP |
| description | Leishmaniasis is a group of diseases that have limited and high toxic therapeutic options. Herein, we evaluated the antileishmanial potential and cytotoxicity of hexanic extract obtained from the Antarctic brown alga Ascoseira mirabilis using bioguided fractionation against Leishmania amazonensis and murine macrophages, which was fractionated by SPE, yielding seven fractions (F1-F7). The fraction F6 showed good anti-amastigote activity (IC50 = 73.4 ± 0.4 μg mL−1) and low cytotoxicity (CC50 > 100 μg mL−1). Thus, in order to identify the bioactive constituent(s) of F6, the fraction was separated in a semipreparative HPLC, yielding four fractions (F6.1-F6.4). F6.2 was the most bioactive fraction (IC50 = 66.5 ± 4.5 μg mL−1) and GC-MS analyses revealed that the compounds octadecane, propanoic acid, 1-monomyristin and azelaic acid correspond to 61% of its composition. These data show for the first time the antileishmanial potential of the Antarctic alga A. mirabilis. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | BR_dfaecd0dba01b8676b8e9a43c66edfcf |
| institution | UNESP |
| instname_str | Universidade Estadual Paulista (UNESP) |
| language | eng |
| network_acronym_str | BR |
| network_name_str | Brasil |
| oai_identifier_str | oai:repositorio.unesp.br:11449/199062 |
| publishDate | 2020 |
| publishDateSort | 2020 |
| reponame_str | Repositório Institucional da UNESP |
| spelling | The antileishmanial activity of the antarctic brown alga Ascoseira mirabilis Skottsberg Clementino, Leandro da Costa [UNESP] Oda, Fernando Bombarda [UNESP] Teixeira, Thaiz Rodrigues Tavares, Renata Spagolla Napoleão Colepicolo, Pio Santos, André Gonzaga dos [UNESP] Debonsi, Hosana Maria Graminha, Márcia A. S. [UNESP] Antarctic macroalgae Ascoseiraceae Brown algae Cutaneous leishmaniasis GC-MS Natural products Leishmaniasis is a group of diseases that have limited and high toxic therapeutic options. Herein, we evaluated the antileishmanial potential and cytotoxicity of hexanic extract obtained from the Antarctic brown alga Ascoseira mirabilis using bioguided fractionation against Leishmania amazonensis and murine macrophages, which was fractionated by SPE, yielding seven fractions (F1-F7). The fraction F6 showed good anti-amastigote activity (IC50 = 73.4 ± 0.4 μg mL−1) and low cytotoxicity (CC50 > 100 μg mL−1). Thus, in order to identify the bioactive constituent(s) of F6, the fraction was separated in a semipreparative HPLC, yielding four fractions (F6.1-F6.4). F6.2 was the most bioactive fraction (IC50 = 66.5 ± 4.5 μg mL−1) and GC-MS analyses revealed that the compounds octadecane, propanoic acid, 1-monomyristin and azelaic acid correspond to 61% of its composition. These data show for the first time the antileishmanial potential of the Antarctic alga A. mirabilis. Chemistry Institute São Paulo State University (UNESP) School of Pharmaceutical Sciences São Paulo State University (UNESP) School of Pharmaceutical Sciences of Ribeirão Preto São Paulo University (USP) Chemistry Institute São Paulo University (USP) http://dx.doi.org/10.1080/14786419.2020.1782403 Natural Product Research. 1478-6427 1478-6419 http://hdl.handle.net/11449/199062 10.1080/14786419.2020.1782403 2-s2.0-85087355972 |
| spellingShingle | The antileishmanial activity of the antarctic brown alga Ascoseira mirabilis Skottsberg Clementino, Leandro da Costa [UNESP] Antarctic macroalgae Ascoseiraceae Brown algae Cutaneous leishmaniasis GC-MS Natural products |
| status_str | publishedVersion |
| title | The antileishmanial activity of the antarctic brown alga Ascoseira mirabilis Skottsberg |
| title_full | The antileishmanial activity of the antarctic brown alga Ascoseira mirabilis Skottsberg |
| title_fullStr | The antileishmanial activity of the antarctic brown alga Ascoseira mirabilis Skottsberg |
| title_full_unstemmed | The antileishmanial activity of the antarctic brown alga Ascoseira mirabilis Skottsberg |
| title_short | The antileishmanial activity of the antarctic brown alga Ascoseira mirabilis Skottsberg |
| title_sort | The antileishmanial activity of the antarctic brown alga Ascoseira mirabilis Skottsberg |
| topic | Antarctic macroalgae Ascoseiraceae Brown algae Cutaneous leishmaniasis GC-MS Natural products |
| topic_facet | Antarctic macroalgae Ascoseiraceae Brown algae Cutaneous leishmaniasis GC-MS Natural products |
| url | http://dx.doi.org/10.1080/14786419.2020.1782403 http://hdl.handle.net/11449/199062 |