Antimicrobial and antibiofilm activities of phenolic compounds extracted from Populus nigra and Populus alba buds (Algeria)
The interest of this work is the discovery of new antimicrobial agents of plant origin to inhibit the formation of microbial biofilms. The present research was conducted to identify and quantify the phenolic compounds extracted from Populus nigra and Populus alba buds harvested in the area of TiziOu...
| Authors: | ; ; |
|---|---|
| Format: | Article |
| Status: | Published version |
| Publication date: | 2019 |
| Country: | Brasil |
| Institution: | USP |
| Repository: | Brazilian Journal of Pharmaceutical Sciences |
| Language: | English |
| OAI Identifier: | oai:revistas.usp.br:article/164813 |
| Online Access: | https://www.revistas.usp.br/bjps/article/view/164813 |
| Access Level: | Open access |
| Keyword: | Populus nigra Populus alba HPLC Polyphenols Antimicrobial activity Antibiofilm activity |
| _version_ | 1878765174608887808 |
|---|---|
| author | Nassima, Boumghar |
| author2 | Nassima, Behidj Riadh, Ksouri |
| author2_role | author author |
| author_browse | Nassima, Behidj Nassima, Boumghar Riadh, Ksouri |
| author_facet | Nassima, Boumghar Nassima, Behidj Riadh, Ksouri |
| author_role | author |
| collection | Brazilian Journal of Pharmaceutical Sciences |
| description | The interest of this work is the discovery of new antimicrobial agents of plant origin to inhibit the formation of microbial biofilms. The present research was conducted to identify and quantify the phenolic compounds extracted from Populus nigra and Populus alba buds harvested in the area of TiziOuzou (Algeria), and to evaluate their antimicrobial and antibiofilm activity. High performance liquid chromatography (HPLC) was performed to identify the phenolic compounds in the ethyl acetate fraction of P. nigra and the methanolic extracts of P. nigra and P. alba. The antimicrobial activity of the crude extracts and the fractions of these two species was tested against 11 microorganisms, using the disk diffusion method, while the antibiofilm effect of certain extracts was carried out in a 96-well microplate and on a biomaterial (catheter). HPLC analysis revealed the presence of 10 bioactive compounds. The main phenolic compounds identified in the three extracts were p-coumaric acid, ellagic acid, and Kaempferol. This study was able to demonstrate that the extracts of P. nigra and P. alba buds have interesting antimicrobial properties, with diameters ranging from 6.6 to 21.3 mm. In addition, extracts of P. nigra exhibited antibiofilm effects greater than 70%. Our results provide evidence for the antimicrobial and antibiofilm potential of bud extracts from both poplar species. Thus, these results will pave the way for further research on these two plants. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | BR_d4e49183e5da5ec611fbd588ecccd421 |
| institution | USP |
| instname_str | Universidade de São Paulo (USP) |
| language | eng |
| network_acronym_str | BR |
| network_name_str | Brasil |
| oai_identifier_str | oai:revistas.usp.br:article/164813 |
| publishDate | 2019 |
| publishDateSort | 2019 |
| publisher | Universidade de São Paulo. Faculdade de Ciências Farmacêuticas |
| publisherStr | Universidade de São Paulo. Faculdade de Ciências Farmacêuticas |
| reponame_str | Brazilian Journal of Pharmaceutical Sciences |
| spelling | Antimicrobial and antibiofilm activities of phenolic compounds extracted from Populus nigra and Populus alba buds (Algeria) Nassima, Boumghar Nassima, Behidj Riadh, Ksouri Populus nigra Populus alba HPLC Polyphenols Antimicrobial activity Antibiofilm activity The interest of this work is the discovery of new antimicrobial agents of plant origin to inhibit the formation of microbial biofilms. The present research was conducted to identify and quantify the phenolic compounds extracted from Populus nigra and Populus alba buds harvested in the area of TiziOuzou (Algeria), and to evaluate their antimicrobial and antibiofilm activity. High performance liquid chromatography (HPLC) was performed to identify the phenolic compounds in the ethyl acetate fraction of P. nigra and the methanolic extracts of P. nigra and P. alba. The antimicrobial activity of the crude extracts and the fractions of these two species was tested against 11 microorganisms, using the disk diffusion method, while the antibiofilm effect of certain extracts was carried out in a 96-well microplate and on a biomaterial (catheter). HPLC analysis revealed the presence of 10 bioactive compounds. The main phenolic compounds identified in the three extracts were p-coumaric acid, ellagic acid, and Kaempferol. This study was able to demonstrate that the extracts of P. nigra and P. alba buds have interesting antimicrobial properties, with diameters ranging from 6.6 to 21.3 mm. In addition, extracts of P. nigra exhibited antibiofilm effects greater than 70%. Our results provide evidence for the antimicrobial and antibiofilm potential of bud extracts from both poplar species. Thus, these results will pave the way for further research on these two plants. Universidade de São Paulo. Faculdade de Ciências Farmacêuticas https://www.revistas.usp.br/bjps/article/view/164813 10.1590/s2175-97902019000218114 |
| spellingShingle | Antimicrobial and antibiofilm activities of phenolic compounds extracted from Populus nigra and Populus alba buds (Algeria) Nassima, Boumghar Populus nigra Populus alba HPLC Polyphenols Antimicrobial activity Antibiofilm activity |
| status_str | publishedVersion |
| title | Antimicrobial and antibiofilm activities of phenolic compounds extracted from Populus nigra and Populus alba buds (Algeria) |
| title_full | Antimicrobial and antibiofilm activities of phenolic compounds extracted from Populus nigra and Populus alba buds (Algeria) |
| title_fullStr | Antimicrobial and antibiofilm activities of phenolic compounds extracted from Populus nigra and Populus alba buds (Algeria) |
| title_full_unstemmed | Antimicrobial and antibiofilm activities of phenolic compounds extracted from Populus nigra and Populus alba buds (Algeria) |
| title_short | Antimicrobial and antibiofilm activities of phenolic compounds extracted from Populus nigra and Populus alba buds (Algeria) |
| title_sort | Antimicrobial and antibiofilm activities of phenolic compounds extracted from Populus nigra and Populus alba buds (Algeria) |
| topic | Populus nigra Populus alba HPLC Polyphenols Antimicrobial activity Antibiofilm activity |
| topic_facet | Populus nigra Populus alba HPLC Polyphenols Antimicrobial activity Antibiofilm activity |
| url | https://www.revistas.usp.br/bjps/article/view/164813 |