Caracterização molecular de plasmídeos de amostras clínicas brasileiras carreadoras do gene QNR
It is very important to know how microorganisms acquire and express resistance phenotype to promote the development of effective strategy for regulating the use of antimicrobial agents commonly used in Brazil. Therefore, the study was designed to investigate plasmideal mechanisms involved in the mai...
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| Tipo de documento: | Dissertação de mestrado |
| Estado: | Published version |
| Data de publicação: | 2016 |
| País: | Brasil |
| Recursos: | UNIFESP |
| Repositório: | Repositório Institucional da UNIFESP |
| Idioma: | Português |
| OAI Identifier: | oai:repositorio.unifesp.br:11600/48813 |
| Acesso em linha: | https://sucupira.capes.gov.br/sucupira/public/consultas/coleta/trabalhoConclusao/viewTrabalhoConclusao.jsf?popup=true&id_trabalho=4988184 http://repositorio.unifesp.br/handle/11600/48813 |
| Nível de acesso: | Acesso aberto |
| Palavra-chave: | Plasmídeos/classificação/genética Enterobacteriaceae Resistência microbiana a medicamentos Grupos de incompatibilidade plasmideal Análise de sequência de DNA |
| Resumo: | It is very important to know how microorganisms acquire and express resistance phenotype to promote the development of effective strategy for regulating the use of antimicrobial agents commonly used in Brazil. Therefore, the study was designed to investigate plasmideal mechanisms involved in the maintenance of resistant bacterial strains at the environment. Plasmids carrying qnr determinants previously reported in bacterial strains isolated in Brazil (n = 19) will be typed and sub-typed by Multiplex PCR and Sequencing by multilocus (pMLST), molecular methods that have not been explored in Brazil, which allow to compare data obtained from strains isolated in various parts of the world. It was possible to determine the incompatibility group from 21 plasmid, as belonging to 7 from 18 groups tested, the groups incN and incFIB present in Escherichia coli, and the incFIIk and incHI2 groups, more frequent in Klebsiella pneumoniae. It was also possible to identify the incompatibility groups in 4 of 8 Enterobacter cloacae tested. IncL/M, incA/C and incHI2 groups were found in these strains. Plasmids incN/STI, incN/ST5, incHI2/ST1, incI1/ST1, incFIIk/ST1 and incFIB/ST1 were identified by analysis of plasmid MLST. IcnN/ST1 plamids in E. coli UNIFAL 79 and E. coli UNIFAL 414 strains and incN/ST5 plasmids in E. coli JF 234 e K. pneumoniae JF 342 strains where associated to qnr genes. The results expand knowledge about types of plasmids involved in these resistance phenotypes, allowing the determination of the epidemiological profile of occurrence of plamideal group?s higher frequency. |
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